Self-referencing method in revised-OTFTOOL is a new method in On-The-Fly(OTF) observation mode. It uses the source free regions of the observed frame as references instead of the OFFs references. We already analyzed and discussed its proprieties and advantages in the previous paper. In this paper, we make a statistical study about the self-referencing method by applying it to OTF mapping data of 27 Virgo spiral galaxies. We found that the self-referencing method solves the crooked baseline problem for every datacube. It straightens the baseline, and conserves the emissions. Compared with other data processing, the median filtering task 'mwflt' in AIPS, to use self-referencing method is more effective and safe not only to straighten the baseline but also to conserve the emission. For the strong CO galaxies, the data obtained by self-referencing method shows scarcely any difference from those reduced by conventional OFFs references and AIPS median filtering in the range of uncertainties. Undetected CO emissions in datacubes of conventional OFFs references are also not detected in those of self-referencing method. The self-referencing method is expected to save the observing time and simplify data reduction processes. Besides this, using self-referencing method will offer emission-free references more safely.