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        검색결과 17

        1.
        2023.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 g/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.
        4,000원
        3.
        2020.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 병원간호사의 간호조직문화, 조직커뮤니케이션만족, 직무착근도의 관계를 확인하고, 직무착근도에 미치는 영향요인을 파악하기 위함이다. C시 소재한 대학병원에 근무하고 있는 간호사 238명을 대상으로 2017년 8월 1일∼8월 10일까지 자료수집 하였다. 자료는 SPSS WIN 22.0 통계 프로 그램을 이용하여, 기술통계, t-test, ANOVA, Pearson’s correlation coefficient, Stepwise Multiple Linear Regression으로 확인하였다. 본 연구결과 간호조직문화는 5점 만점에 평균 3.32±0.33점 이었고, 조직커뮤니케이션만족은 5점 만점에 평균 3.19±0.37점 이었으며, 직무착근도는 5점 만점에 평균 3.19±0.44점이었다. 일반적 특성에 따른 직무착근도의 차이는 연령(F=9.00, p<.001), 학력(F=13.24, p<.001), 직위(t=-5.42, p<.001), 결혼(t=-3.11, p=.002), 근무경력(F=10.55, p<.001), 근무형태 (F=10.99, p<.001)에서 유의한 차이가 있었다. 직무착근도와 간호조직문화(r=.502, p<.001), 조직커뮤니케이션만족(r=.673, p<.001)은 양의 상관관계가 있었다. 직무착근도에 영향을 미치는 요인으로 조직커뮤 니케이션만족(β=.575 p=.000), 근무경력 ‘10년 이상’(β=.342 p=.000), 간호조직문화(β=.203 p=.000), 근무경력 ‘5년 이상∼10년 미만’(β=.121 p=.000)의 순이었고, 모형의 설명력은 57.4%이었다. 이상의 결과에 따라 병원간호사의 간호직무에 착근도를 높이기 위하여 조직커뮤니케이션만족도를 수시로 파악하고, 근무경력, 간호조직문화 등을 고려한 간호실무 및 교육중재 프로그램 개발이 필요하다.
        4,200원
        4.
        2018.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 연잎, 상엽, 건조 무 혼합 추출물의 wogonin, quercetin, quercetin-3-O-glucuronide의 함량 분석 및 해당 표준물질들의 동시 분석법의 개발 및 검증을 실시하였다. 분석법은 특이성, 직선성, 정확성, 정밀성, 검출한계 및 정량한계를 통하여 분석법의 신뢰성을 검증했다. HPLC를 이용한 분석방법에서 표준용액의 머무름 시간과 연잎, 상엽, 건조 무 혼합 추출물 중 quercetin-3-O-glucuronide의 머무름 시간이 일치하였으며 동일한 spectrum을 나타내는 것을 확인해 분석법의 특이성을 검증하였다. Wogonin, quercetin 및 quercetin-3-O-glucuronide의 검량선은 상관계수 값이 wogonin, quercetin 및 quercetin-3-O-glucuronide 모두 0.9999로 우수한 직선성을 보여 분석에 적합함을 알 수 있었고 정밀성과 정확성은 농도를 아는 시료에 인위적으로 저농도, 중농도, 고농도의 표준물질을 첨가해 계산하였다. Wogonin, quercetin, quercetin-3-O-glucuronide의 정밀성은 일간 정밀성, 일내 정밀성으로 확인했으며 세 가지 성분들의 일간 정밀성은 각각 0.76~1.24%, 0.90~1.40%, 0.74~1.87% 수준으로 확인되었고, 일내 정밀성은 0.12~1.06%, 0.28~1.12%, 0.52~0.92%로 5% 이하의 우수한 정밀성을 보였다. 정확성의 측정 결과 wogonin, quercetin 및 quercetin-3-O-glucuronide의 일간 정확성은 99.99~107.97%, 99.96~115.88%, 99.73~111.91%를 나타냈고 일내 정확성은 100.36~108.88%, 99.77~114.81%, 99.91~112.44%로 우수한 수준의 정확성을 보였다. Wogonin, quercetin 및 quercetin-3-O-glucuronide의 검출한계는 각각 0.16 μg/mL, 0.09 μg/mL, 0.15 μg/mL로 측정됐으며 정량한계는 각각 0.48 μg/mL, 0.26 μg/mL, 0.45 μg/mL로 나타나 저농도에서도 검출이 가능함을 확인할 수 있었다. 분석법 검증결과, 확립된 분석법은 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계가 모두 우수한 분석법임이 검증됐으며, 검증된 분석법을 이용해 연잎, 상엽, 건조 무 혼합 추출물의 wogonin, quercein, quercetin-3-O-glucuronide의 함량을 분석한 결과 wogonin과 quercetin은 검출이 되지 않았지만 quercetin-3-O-glucuronide는 함량을 가지고 있는 것으로 확인됐다. 본 연구 결과 에탄올로 추출한 11번(EM11) 샘플에서 quercetin-3-O-glucuronide의 함량이 가장 높게 나왔으며 열수와 에탄올로 추출한 샘플들이 함유하고 있는 quercetin-3-Oglucuronide의 함량은 불검출~50.95 mg/dry weight g 수준으로 함유되어 있는 결과를 보였다.
        4,000원
        5.
        2016.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        We analyzed research trend and public perception related with tap water to identify major factors affecting low consumption of tap water. 805 research articles were collected for text mining analysis and 1,000 on-line questionnaires were surveyed to find social variables influencing tap water intake. Based on the word network analysis, research topics were divided into 4 major categories, 1) drinking water quality, 2) water fluoridation, 3) residual chlorine, and 4) micro-organism management. Compared with these major research topics, scientific studies of drinking behavior, or social perception were rather limited. 22.4% of total respondents used tap water as drinking water source, and only 1% drank tap water without further treatments (i.e. boiling, filtering). Experience of quality control report (B=0.392, p=0.046) and level of policy trust (B=1.002, p<0.0001) were influential factors on tap water drinking behavior. Age (B=0.020, p=0.002) and gender (B= - 1.843, p<0.0001) also showed significant difference. To increase the frequency of drinking the tap water by social members, the more scientific information of tap water quality and the water policy management should be clearly shared with social members.
        4,000원
        8.
        2019.07 KCI 등재 서비스 종료(열람 제한)
        Purpose - Previous studies on the influence of appeal types of advertising messages on pro-social behavior have shown inconsistent results. The purpose of this study is to examine whether the appeal type of advertising affects consumer’s prosocial behavior. In particular, the authors investigated whether the social exclusion moderates the relationship between the appeal type of advertising and prosocial behavior. Research design, data and Methodology - To verify the hypothesis, two experimental study were conducted. The experimental study was 2(appeal type : self-benefit vs. other-benefit) by 2(social exclusion : being ignored vs. being rejected) between-subject design. Participants were randomly assigned to one of four conditions. The experiment was conducted using a computer. First, the participants was exposed to the advertising image used in the social campaign, and then responded to questions about social behavior. The participants completed the experiment after further responding to the item for manipulation check and demographic measure. The experiment took about 10 minutes in total. Results - In Study 1, a global warming campaign with participation intention variable, other-benefit ads rather than self-benefit ads drive consumers who feel socially rejected to be more willing and likely to participate, but consumers who feel socially ignored are more likely to participate the campaign in self-benefit ad than other-benefit ad. Study 2 replicates the findings, a child poverty relief campaign with an amount-to-donate variable: consumers who feel explicitly rejected allocate more dollars to the charity in response to other-benefit rather than self-benefit ads, but consumers who being ignored are willing to donate more money. Conclusion - This study has theoretical contribution in that it expands existing theories by explaining the existing inconsistent results of the message appeal influence on pro-social behavior through new control variables. In addition, the results have important managerial implications, suggesting marketers should tailor their marketing message to match the consumer situation.
        9.
        2016.01 KCI 등재 서비스 종료(열람 제한)
        We investigated optimal conditions for the hydrolysis of Laminaria japonica using a single enzyme such as Celluclast 1.5 L, Saczyme, and alginate lyase, for the production of reducing sugar. Redesigned experimental conditions including the optimal conditions determined for the single enzyme were proposed, and the hydrolysis of Laminaria japonica was also performed with a mixture of enzymes. The reducing sugar yield with the mixed enzymes was lower than that with Celluclast 1.5 L, which showed the highest efficiency among the enzymes used. Considering the reducing sugar yield and economics, it would seem that hydrolysis by mixed enzymes had no advantage. The coefficient of determination (R2) of Y1 (the yield of reducing sugar by Celluclast 1.5 L) was 0.89. The P value of Y1 was < 0.001, indicating statistical significance. By the response surface methodology (RSM), the optimum reaction conditions for hydrolysis of Laminaria japonica by Celluclast 1.5 L were determined to be enzyme of 8.0%, a reaction time of 26.4 h, a pH of 4.0, and a temperature of 42.6oC, resulting in the production of 117.7 mg/g-Laminaria japonica.
        10.
        2015.01 KCI 등재 서비스 종료(열람 제한)
        Bioethanol was produced from Laminaria japonica hydrolystaes by sequential acidic (0.108 N HCl)/distilled water and enzymatic hydrolysis (Celluclast® 1.5 L) using Saccharomyces coreanus immobilized into/on aluminum silicate. Reducing sugar were hydrolyzed 140.5 and 122.7 mg/g-dry biomass under a acidic-enzymatic condition and a distilled waterenzymatic condition, respectively. In addition, the 8 repetition batch fermentations were carried out with the immobilized S. coreanus to verify the advantage of immobilization cell. As a result, we can obtain the ethanol of 12.1 ~ 24.3 mg/gdry biomass, and reuse the support, aluminium silicate, for 8 repetition batch fermentations without any breakdown.
        11.
        2014.12 KCI 등재 서비스 종료(열람 제한)
        In this study, microcrystalline cellulose, which is a cell wall polysaccharide commonly contained in sea algae (brown algae, red algae and green algae), is used in substitution for cellulose and is hydrolyzed with seven enzymes available in the market. The seven enzymes selected are Viscozyme® L, Celluclast® 1.5 L, Saczyme, Novozym® 33095, Fungamyl® 800 L, Driselase® Basidiomycetes sp., and Alginate Lyase. To maximize the production of the reducing sugar by hydrolysis with each enzyme, we optimized the quantity of enzymes, reaction time, pH, and reaction temperature as four independent variables, and the reducing sugar production rate as a dependent variable, utilizing response surface methodology (RSM) to optimize the enzyme hydrolysis reaction conditions. Among the tested enzymes, the production rate of reducing sugar by Celluclast® 1.5 L was the highest. Hence, the predicted optimum conditions (8.5 % enzyme, reaction time 27.6 h, pH 4.1 and reaction temperature 44.1oC) were directly applied to Laminaria japonica and proved the predicted optimum conditions with experiments. Under the optimum conditions, the sugar yield of 137.6 mg/g-Laminaria japonica (experimental value) was obtained.
        12.
        2014.10 KCI 등재 서비스 종료(열람 제한)
        This study investigated the optimal sequential hydrolysis conditions by comparing with reducing sugar yield ofsequential hydrolysis of Laminaria japonica processing residue. After acid-catalyzed hydrothermal hydrolysis, sequentialenzymatic hydrolysis was performed with single enzymes such as Celluclast® 1.5L, Saczyme, and Alginate Lyase, andtheir mixture. As a result, the yield of reducing sugar by sequential hydrolysis with the mixed enzymes was the highest,but there would be an economical problem with excessive enzyme loading. Therefore, considering the reducing sugaryield and economics, it is thought that hydrolysis by the mixed enzymes has no advantage, thus, using the Celluclast®1.5L in the sequential hydrolysis was practically more appropriate. The optimal sequential hydrolysis conditions ofLaminaria japonica processing residue were determined to be 8% v/w of enzyme injection, 42.6oC of reaction temperature,pH 4.1, and 26 hours of reaction time after acid-catalyzed hydrothermal hydrolysis (0.108 N-HCl, 144oC of reactiontemperature, and 22 minute of reaction time).
        13.
        2013.12 KCI 등재 서비스 종료(열람 제한)
        To investigate optimal condition of acid-catalyzed hydrothermal hydrolysis of Laminaria japonica, the main constituentsof Laminaria japonica such as cellulose, alginic acid and mannitol were hydrolyzed using acid-catalyzed hydrothermalreaction. Then, we proposed the re-designed experimental method including the predicted optimal conditions of the mainconstituents and performed acid-catalyzed hydrothermal hydrolysis of Laminaria japonica. The coefficients ofdetermination (R2) of Y5 (yield of reducing sugar from Laminaria japonica) were 0.877. P values of Y5 were 0.002,indicating significance, within 1% (p<0.01). The optimum reaction condition for acid-catalyzed hydrothermal hydrolysisof Laminaria japonica determined by the response surface methodology is 143.65oC of reaction temperature, 22min ofreaction time, hydrochloric acid concentration 0.108N, resulting in a production rate of 115.62mg/g-Laminaria japonica.
        14.
        2013.06 KCI 등재 서비스 종료(열람 제한)
        This study aimed to investigate the optimal enzymatic hydrolysis conditions of alginic acid using Viscozyme® L, Celluclast® 1.5L, Saczyme®, Novozym®, Fungamyl® 800L, Driselase® Basidiomycetes sp., and Alginate Lyase, for production of reducing sugar. Response surface methodology (RSM) based on central composite rotatable design was used to study effects of the independent variables such as enzyme (1-9% v/w), reaction time (10-30 h), pH (3-7) and reaction temperature (30-70oC) on the production of reducing sugar from alginic acid. The coefficients of determination (R2) of Y1, Y2, Y3, Y4, Y5, Y6, and Y7 for the dependent variable regression equation were analyzed as 0.947 0.968, 0.840, 0.926, 0.923, 0.892 and 0.825. And the p-value of Y1, Y2, Y3, Y4, Y5, Y6, and Y7 within 1% (p < 0.01) was very significant. The optimal conditions were 1.0% of the quantity of the enzyme, 10.0 hours of the response time, pH 3 and 70.0oC of the reaction temperature, where the production rate was 483.1 mg/g-alginic acid, the highest of all the enzymes used.
        15.
        2013.03 KCI 등재 서비스 종료(열람 제한)
        Acid hydrolysis of cellulose using hydrothermal reaction was conducted to maximize reducing sugar concentration and the response surface methodology (RSM) was applied to study the effects of independent variables, such as reaction temperature (116 ~ 184oC), reaction time (12 ~ 28 min) and hydrochloric acid concentration (HCl, 0.0159 ~ 0.1841 N) on reducing sugar concentration and production yield from the cellulose. With the optimum conditions of the acid-catalyzed hydrothermal hydrolysis, the reducing sugar (RS) was obtained as 369.14 mg-RS/g-cellulose in 172.77oC of the reaction temperature, 28.41 min of the reaction time and 0.067 N of the hydrochloric acid concentration. The glucose (Glu) was obtained as 281.94 mg-Glu/g-cellulose in 154.70oC of the reaction temperature, 11.59 min of the reaction time and 0.184 N of the hydrochloric acid concentration.
        16.
        2013.02 KCI 등재 서비스 종료(열람 제한)
        The relationship between debris flow and topographical factors is essential for the reliable estimation of soil loss. The objective of this paper is to estimate stability index and soil loss for assessing landsliding risk caused by debris flow. SIMAP and RUSLE are used to estimate stability index and soil loss, respectively. The landsliding risk area estimated by using SIMAP is found to be different from the large land area estimated by RUSLE. It is found that the spatial distribution of soil cover significantly influences landsliding risk area. Results also indicate that stability index and soil loss, estimated by soil cover factor, improve the assessment of landsliding risk.
        17.
        2012.07 KCI 등재 서비스 종료(열람 제한)
        Sugar, starch and lignocellulosic biomass has been mainly used as raw materials for the production of the bioethanol. However, the sharp fluctuation of grain prices, a threat of world famine, and hardly biodegradable substance like lignin contained in lignocellulosic materials make the pre-processing of the biomass complicated in several aspects. As a result, the focus of attention has now shifted to the ‘third biomass’ such as algae, which has a high value of energy recovery. In this study, a kind of macroalgae and its characteristic were surveyed and then, the physical, biological, chemical, combined, and hydrothermal pretreatments for its hydrolysis were deeply considered. Consequently, the macroalgae could be more effectively hydrolyzed at the combined process such as the hydrothermal-chemical or biological treatment, chemical-biological treatment and so on than the single process like the biological pretreatment.