Background: Inspiratory muscle training can improve inspiratory strength and endurance through threshold loading. In addition, trunk stabilization exercises can improve trunk strength and respiratory function. Objectives: The purpose of this study is to investigate the effect of application of inspiratory muscle training and trunk stabilization exercise on pulmonary function and inspiratory muscle activation in college students. Design: Randomized controlled trials. Methods: In this study, 24 college students were randomly divided into two groups: inspiratory muscle training and trunk stabilization exercise (experimental group, n=12), and trunk stabilization exercise (control group, n=12). Inspiratory muscle activity was measured using a surface electromyography. Pulmonary function was measured using a spirometer and a peak expiratory flow meter. Results: In the experimental group, the muscle activity of both upper trapezius and latissimus dorsi muscles increased significantly after the intervention. In the experimental group, both upper trapezius muscle activity was significantly increased than in the control group. In the experimental group, all the pulmonary function significantly increased after the intervention. Conclusion: In this study, when the inspiratory muscle training was additionally applied to the trunk stabilization exercise in college students in their twenty, it was possible to improve the inspiratory muscle activity and pulmonary function.