본 연구에서는 훈제오리 슬라이스에서 Escherichia coli 유통 중 생장 예측을 위한 dynamic model을 개발하였다. E. coli는 2개의 훈제 오리 시료(16.7%) 에서 1.23 log CFU/ g검출되었다. 10-30oC 보관에 따라 E. coli의 μmax는 0.05- 0.36 log CFU/g/h, LPD는 4.39-1.07h, h0 값은 0.24-0.51을 나타내었다. 개발된 모델의 검증은 15oC, 23oC에서 수행 하였다. 모델 검증 결과 RMSE값이 0.130으로 개발된 모델이 다른 온도에 적용하기에 적합하다고 판단하였다. 이 러한 결과는 E. coli로 개발된 모델은 훈제오리 슬라이스에서 E. coli의 변화하는 온도에 따른 생장을 예측하는 데 유용하다.

The objective of this study was to investigate the expression of bovine luteum expressed sequence tags (ESTs), vascular endothelial growth factor (VEGF), and tumor necrosis factor receptor 1 (TNFR1) and the presence of functional ESTs in the bovine corpus luteum (CL) during different stages of the estrus cycle. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a difference in the expression of ESTs during the CL stage. Concentration of ESTs in the CL tissue increased significantly from the mid-luteal stage and decreased thereafter. RT-PCR analysis showed higher levels of the EST genes in the CL of the mid-luteal stage than in other stages, and the same level of expression of VEGF. Immunohistochemistry analysis of the tissue from CL formation to regression showed low cytosol and aggregation of the nucleus. And activity caspase 3 (apoptosis detector) was most strongly detected in the CL1 stage of bovine. During the estrous cycle, the cytosol was magnified and differentiation of the nucleus was clearly manifested. The ESTs affected the CL, and the relationship between VEGF and TNFR1 played a pivotal role for CL development and activation, dependent on the stage of CL. These results suggest local production of ESTs, the presence of functional ESTs in the bovine CL, and that ESTs play a role in regulating the function of cell death in bovine CL.