This study aims to investigate the physicochemical properties of meat analogs by using high and low moisture extrusion processes to create a plant-based analog burger patty material. The isolated soy protein blends of low- and high-meat analogs (LMMA and HMMA) were texturized using the twin-screw extruder equipped with a cooling die. The highest hardness, cohesiveness, chewiness, and cutting strength were observed in beef, but the highest stringiness was indicated in HMMA. The highest integrity index was seen in beef, while LMMA had the highest nitrogen solubility index (NSI). LMMA also had the highest water holding capacity (WHC) and water absorption capacity (WAC), whereas beef had the highest oil absorption capacity (OAC). LMMA had the highest emulsifying activity (EA) and emulsion stability (ES) in emulsifying properties. Further, the highest protein digestibility was revealed in LMMA. This study suggested that extrusion process types influence the quality of meat analog, which could be the elementary source for manufacturing the analog burger patty.

The objective of this study is to determine the effects of barrel temperature and moisture content on the physicochemical properties of texturized vegetable protein (TVP). The water absorption index, water solubility index, texture, integrity index, color, and scanning electron micrograph of the TVP were analyzed. The extrusion conditions consisted of barrel temperature (110, 130, and 150oC) and moisture content (40, 45, and 50%) at a fixed screw speed of 250 rpm. The TVP extruded at 150oC barrel temperature and bearing 50% moisture content had higher water absorption index and water solubility index. Elastic force, cohesiveness, and color differences were the highest in the TVP extruded at 150oC barrel temperature and possessing 40% moisture content. However, the TVP at 150oC barrel temperature and having 40% moisture content had a lower integrity index than the TVP carrying moisture contents of 45 and 50%. The structure of the TVP extruded at 150oC barrel temperature and having 40% moisture content was found similar to a chicken breast tissue’s structure. In conclusion, 150oC barrel temperature and 40% moisture content are optimal conditional characteristics for the texturization of soy protein isolate and gluten.

This study was carried out using Celluclast 1.5L to increase the content of 2,6-DMBQ and water extractable arabinoxylan in wheat germ extract. Extraction temperatures were 30oC, 45oC and 60oC. The extraction times were 0, 6, 12, 18, 24 and 30 h. The pH of the extract decreased rapidly from 18 h at 30oC in both water- and enzyme-treated extracts. 2,6-DMBQ of water- and enzyme-treated extracts increased with the extraction time. At 30-hour extraction time, enzyme-treated extract increased 27.60% at 30oC extraction temperature than water extraction. Extraction temperatures of 45oC and 60oC were increased by 65.03% and 151.05%, respectively. The highest content of waterextractable arabinoxylan was 15.23±0.08 mg/g when the enzyme was treated at an extraction temperature of 60oC for 30 h. At 30=hour extraction time, enzyme-treated extract increased 7.92% at 30oC extraction temperature compared to water extraction. Extraction temperatures of 45oC and 60oC were increased by 31.20% and 54.38%, respectively.

The aim of this study was to determine the effects of different soy protein concentrate (0, 15, 30, and 45%), defatted soy flour (0, 10, 20, and 30%), and wheat flour (10, 20, and 30%) contents replacing fish meal on physiochemical properties of extruded aquatic feed by using a twin screw extruder. The moisture content, barrel temperature, die diameter, and screw speed were adjusted to 45%, 137oC, 4 mm, and 250 rpm, respectively. With the higher amount of soy protein concentrate replacing fish meal, the expansion ratio, swelling ratio, and water stability increased significantly while specific length and water holding capacity decreased significantly. With the increasing defatted soy flour content, expansion ratio increased significantly, but specific length, water holding capacity and water stability decreased significantly. With increase in the wheat flour content, the specific length significantly decreased while the water stability significantly increased. In conclusion, aquatic feed quality was optimized in this study and could be used in the future.

This study focuses on evaluating the physico-chemical properties of extruded cassava starch added with corn flour. The raw materials were mixed at different ratios (corn flour/cassava starch 0:100, 25:75, 50:50, 75:25, 100:0 [w/w]), then extruded at different barrel temperatures (120 and 140°C) and moisture contents (20 and 25%), with the physicochemical and pasting properties of extrudates finally analyzed. The obtained results showed that the addition of corn flour to the mix resulted in an increase in specific length, lightness, yellowness, water absorption index, water solubility index, final viscosity, and setback viscosity of extrudates, while there was a decrease in redness and piece density. Other properties including the expansion ratio, reducing sugars, cold viscosity, peak viscosity, hold viscosity, and breakdown viscosity were more likely to be subjected to barrel temperature and moisture content. Mixing corn flour with cassava starch ameliorated the expansion properties, color, and some pasting properties of mixed extrudates. This study demonstrated that the use of corn flour and extrusion process with different barrel temperatures and moisture contents provided valuable data for the further development of extruded cassava starch.

The aim of this research is to evaluate the impact of various process conditions on the physical properties and digestibility of extruded fish feed at different barrel temperatures (120, 130, and 140oC) and moisture contents (35, 40, and 45%). The expansion ratio decreased with increasing the moisture content. The hardness was negatively correlated with the expansion ratio. The bulk density decreased by increasing moisture content. The water holding capacity increased by increasing the moisture content and barrel temperature. Soaking time significantly (p<0.05) affected water stability and the best water stability was observed in all samples soaked for 1 h, but water stability was also stable at 2 h and 3 h. There was a significant increase in the protein digestibility of extruded fish feed, compared to that of the raw material.

This study was to demonstrate the comparison of immunological activity between vegetable soup made by fresh and extruded radish in in-vitro (bone marrow-derived macrophages and dendritic cells, and mouse splenocytes) and invivo models. In cell survival tests, extruded radish added to vegetable soup (EVS) and non-extruded radish added to vegetable soup powder (NEVS) were treated with bone-marrow derived macrophages, dendritic cells, and mouse splenocytes, and showed no cytotoxic effect at a dose below 1000 μg/mL. EVS treated cells had greater cell proliferation and cytokine [tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, IL-2, interferon (INF)-γ] production when compared to the NEVS treated group. Cell surface marker (CD 80/86, MHC class I/II) expression in bone marrow-derived macrophages and dendritic cells was evenly increased in the EVS treated group. In in-vivo study, administration of EVS increased for the CD4 and CD8 T cell population in splenocyte and cytokine production (IL-2, IFN-γ, TNF-α) but not Th2 type cytokines (IL-4). Therefore, adding the extruded radish is a more effective method for vegetable soup to increase immunological activity against immune cells.

This study was carried out to determine the effect of biji addition on the quality of extruded isolated soy protein (ISP). ISP-based biji addition (0, 20, 40 and 60%) was extruded at die temperatures 140oC and 150oC and a screw speed of 250 rpm. The moisture contents were adjusted to 35% and 45%. As the content of biji increased from 0% to 60%, springiness, cohesiveness, and cutting strength of the extruded ISP were significantly decreased. When the die temperature increased from 140oC to 150oC and the water content increased from 35% to 45%, the nitrogen solubility index, cutting strength and integrity index decreased. When the biji addition was increased at a barrel temperature of 140oC and a moisture content of 45%, the integrity index of the ISP texture decreased. With an increase in the addition of biji to ISP, rough surface and irregular fiber structure were observed, but regular microfiber structure was not observed.

The present study aimed to investigate the effect of fermentation on physical properties of texturized vegetable protein (TVP) by using Bacillus subtilis. TVP was prepared by texturizing the mixture of isolated soy protein (50%), wheat gluten (40%) and corn starch (10%) with the use of twin-screw extruder at two different moisture contents (40% and 50%), 100 g min-1 feed rate, 200 rpm screw speed and 130°C die temperature. Then, TVPs were subjected to solid-state fermentation using B. subtilis at 37°C for 60 h. The texture (springiness, cohesiveness, chewiness and hardness), integrity index and color (lightness, redness and yellowness) of TVPs were determined before and after fermentation for 0, 12, 24, 36, 48 and 60 h, respectively. The springiness and cohesiveness did not change significantly after fermentation in all samples. However, chewiness and hardness decreased with increased fermentation time with the lowest values observed at 60 h after fermentation of TVPs (both 40% and 50% MC). Integrity index of fermented TVPs was significantly reduced while fermentation time was increased. Integrity index started to decrease significantly at 36 h after fermentation in TVP extruded with 40% MC. In another TVP (50% MC), the integrity decreased at 24 h after fermentation but it maintained higher integrity index than TVP (40% MC) throughout the whole fermentation period. The significant total color change was observed with increased fermentation time. During fermentation, lightness and yellowness decreased but redness increased significantly. This study pointed out that TVP fermented by B. subtilis still has good texture, and thus has the potential to become an attractive food for consumers who usually avoid fermented foods due to rotten appearance with no texture.

Chemical cross-linking of different plant protein-based meat analogues was examined based on protein solubility of 8 different buffer solutions. The specific chemical bond and their interactions were further analyzed. Isolated soy protein (ISP), mung bean protein (MBP), peanut protein (PNP), pea protein (PP) and wheat gluten (WG) were texturized using a co-rotating twin-screw extruder at 50% moisture content. The results showed that protein solubility of meat analogues significantly decreased after extrusion, compared to their raw materials (P<0.05). The protein solubility of meat analogues increased with increasing reagent in buffer solutions. Hydrophobic interactions, hydrogen bonds, disulfide bonds and their interactions were found in the structure of meat analogues. The highest amount of covalent bond was observed in PP-meat analogues followed by ISP, WG, PNP, and the lowest MBP-meat analogues. The study demonstrated that PP are valuable raw materials for the development of meat analogue, which could promote high cross-linking bonds.

The objective of this study was to determine the effects of moisture content and screw speed on the physical properties of high moisture meat analogue. Extrusion conditions were moisture content (65, 70%), screw speed (150, 200 rpm), a fixed barrel temperature (160℃), and feeder rate (100 g/min). Specific mechanical energy (SME) input decreased as moisture content increased from 65 to 70%. The high moisture meat analogue at 65% moisture content and 150 rpm had higher water absorption capacity, elasticity, cohesiveness, chewiness and cutting strength than those of high moisture meat analogue at 70% moisture content and 150 rpm. However, the high moisture meat analogue at 70% moisture content and screw speed 200 rpm had a lower integrity index, elasticity, cohesiveness, chewiness and cutting strength than those of high moisture meat analogue at 65% moisture content and 200 rpm. In conclusion, the tested physical properties of high moisture meat analogue were more affected by moisture content than screw speed.

This study aims to investigate the physicochemical properties of extruded Biji. As the extrusion process variables, the barrel temperature and the moisture content were adjusted at barrel temperatures of 120, 140, and 160°C and 35, 45% respectively. L-value (lightness) increased as the moisture content and barrel temperature increased from 35% to 45% and 120°C to 160°C. In contrast, decreasing a-value and b-value resulted in increasing moisture content and barrel temperature. Total sugar, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity, and total phenolic compound were increased by the extrusion process. The water solubility index (WSI) and dietary fiber of the extruded biji decreased to 3.9% and 592.6 mg/g compared to the non-extruded biji of 12.3% and 592.6 mg/g. According to the result of this study, the extruded biji could be used in various kinds of food processing, and future study is needed to find the optimum condition of the extrusion.

This study investigates the quality characteristics of dough and bread added with 6% chestnut shell powder and extruded chestnut shell powder at various conditions. As extrusion process variables, melt temperature (110°C, 130°C, 150°C) and moisture (25% and 30%) were controlled. Total dietary fiber content was slightly increased in extruded chestnut shell powder group. In the farinogram, absorption was significantly increased in the group of 25% moisture content and 30% moisture content (p<0.05). After 2 hours and 3 hours, the leavening heights of dough for control showed a similar tendency to that of dough with extruded chestnut shell at a melt temperature 150°C and with moisture content of 25% and 30%. Specific volume was the highest at a control of 3.74±0.08 cc/g and extruded chestnut shell powder group was slightly higher than the chestnut shell powder group. Firmness after 1 day on control of 107.42±14.52 g was similar to that of the bread with extruded chestnut shell at a temperature of 150°C and moisture content of 25% for 113.33±6.17 g. In conclusion, the extrusion-cooking of chestnut shell powder improved the quality characteristics of dough and bread. The optimum combinations of conditions in tested range were melt temperature at 150°C and moisture content at 25%, and melt temperature at 130°C and moisture content at 30%.

The comparison of physicochemical properties and cell structure of texturized vegetable protein (TVP) and different types of meats (beef, pork, and chicken samples) were studied. TVP from the blend of 40% isolated soy protein (ISP) and 60% wheat gluten (WG) was texturized with a twin-screw extruder at 100 g/min feed rate, 45% moisture content, 250 rpm screw speed and 140℃ die temperature. The nitrogen solubility index, integrity index, chewiness, longitudinal cutting strength and microstructure of TVP were similar to the chicken sample, when compared to the other meats. However, water absorption capacity (WAC) of TVP (217.38%) was significantly (P<0.05) higher than those of meat samples (109.53 to 165.35%). The microstructure of TVP showed the fibrous structure. Non-uniform air cells were observed in the microstructure of TVP, which could indicate high WAC. The study demonstrated that the physicochemical and cell structure of TVP from the blend 40% ISP and 60% WG with 45% moisture extrusion process was similar as chicken sample.