The objective of the study was to identify 52 Asian pear accessions, two primary pear species, and one reference pear Asian pear with 12 microsatellite markers to maintain pear germplasm collection. The number of alleles of 12 microsatellites detected ranged from eight at CH03d12 to 18 at CH01f07. Gene diversity ranged from 0.7053 at CH01d08 to 0.9224 at CH01f07. The lowest value of PIC was 0.6600 at CH01d08 and the highest was 0.9171 at CH01f07. A group consisting of ‘Ooharabeni,’ ‘Bartlett,’ and P. calleryana was out-grouped and served as a reference to determine the relationship among Asian pear accessions. Except for the out-group, 50 Asian pears were segregated into two groups. Group I was divided in two small groups. Each small group was characterized by P. bretschneideri and P. ussuriensis, respectively. Group II was characterized as P. pyrifolia, and the group was divided in four small groups. The eigenvalue, difference, proportion, and cumulative of six principal components based on PCA to 12 microsatellite. The eigenvalue of the first principal components was 5.5850. The proportion of the first principal component was 0.9308. The cumulative value of the first two principal components was 0.9801. Consequently, nearly all of the results were elucidated by the two principal components. The results from analysis of the standard set of microsatellites in this study may be used as basic materials for the management of Asian pear germplasm collections, and the data might be useful in the development of a core collection.