An experiment was conducted to study effects of abomasal infusions of casein, glucose or starch on milk production and blood metabolites in dairy cows. Four lactating cows (559±41.9kg) fitted with 100 mm ruminal cannulas were used in a 4 × 4 Latin square experiment. The cows were given access ad libitum to grass silage and received 6.2 kg dry matter (DM) of a mixture of rapeseed meal (5.3kg/d DM) and barley grain (0.9kg/d DM) as a basal diet (CON), the basal diet plus intra-abomasal infusions of 270 g sodium caseinate (CAS), 300 g of glucose (GLU) or 243 g of starch (STA) starting at 09:00 h each day. Abomasal infusion of different nutrients did not affect (p>0.05) grass silage intake and rumen fermentation parameters (rumen pH, ammonia N and volatile fatty acids). As unexpected, milk yield and composition data did not differ between CON and the treated groups (p>0.05). Most of plasma amino acids were not significantly (p>0.05) affected by the abomasal infusions of CAS, GLU or STA except for histidine, tryptophan and glycine. Although histidine, known as the first limiting amino acid in dairy cows fed grass silage based diets, was significantly (p=0.003) increased by CAS, we failed to result in a clear increase in milk yield and protein yield. This discrepancy between plasma AA profiles and milk production could be presumably explained by relatively high concentration of total amino acids in the present study (2,039μmol/l).

A survey project of TRAO with the fifteen beam array receiver system is presented. A multibeam array receiver system has been purchased from FCRAO, and is being installed on TRAO 14m telescope. The target region of the survey is from ι=120° ~137°, b=-1°~+1°, and velocity resolution would be 1 km/sec after smoothing from the original resolution of 0.64km s-1in the transition of J = 1-0 of 13CO The survey region is a part of the 12CO Outer Galaxy Survey(OGS), and would be an extension of the Bell Laboratories 13CO Galactic Plane Survey. By combining with the existing 12CO database of the Outer Galaxy Survey, we will derive physical properties of identified molecular clouds and will conduct and statistical analysis of the Outer Galalxy molecular clouds. Reduction process and analysis methods will be introduced.