This study aimed to investigate antioxidant activity of various extracts from fruiting bodies and mycelia of two Phellinus linteus strains and P. baumii. The Phellinus strains have cultivated on oak and mulberry logs. The fruiting bodies species were harvested from each Phellinus strain and used in this study. The tested items include: 2, 2’-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), free radical scavenging assay and determination of total phenolics contents (TPC), ferric reducing antioxidant power assay (FRAP), and DPPH (1, 1-diphenyl-2- picrylhydrazyl) radical-scavenging activities. Different extractions with 60% Ethyl alcohol, 70% methyl alcohol and heat water were done on the mycellial and fruiting bodies samples of the mushroom species. The methyl alcohol extraction from fruiting body of P. linteus KACC93057P displayed the highest antioxidant activity on ABTS, FRAP, and DPPH assays. The ethyl acetate fraction was concentrated and subjected to an ODS column chromatography, followed by Sephadex LH-20 column chromatography. Finally six compounds 1-6 were detected by preparative reversed-phase HPLC.