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        검색결과 29

        3.
        2018.12 KCI 등재후보 구독 인증기관 무료, 개인회원 유료
        This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative hydroxychloroquine-D4 as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column (50 mm × 4.6 mm, 2.6 μm) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 – 500 ng/mL for HCQ; 2 – 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.
        4,000원
        4.
        2017.03 구독 인증기관 무료, 개인회원 유료
        투과와 반사 타입의 네 가지 홀로그램을 동일한 공간에서 촬영할 수 있는 하이브리드 기록 시스템에 대해 연구하였다. 하이브리드 기록 시스템은 빔 스프린트와 스페셜 필터, 셔터를 적재적소에 설치해 간섭무늬를 만드는 빛 의 경로를 변화시켜 홀로그램을 촬영할 수 있게 했다. 기록 시스템은 노광 공간을 최소화 하였고 홀로그램 카메라로 적용할 수 있게 했다. 프레넬 홀로그램은 오브젝트를 좌우 방향에서 조명하여 고효율의 입체 영상이 표시되게 제작했 다. 리프만 홀로그램은 프레넬 홀로그램에서 공액으로 재생 된 실상을 이용하여 촬영했다. 데니슈크 홀로그램은 기록 재료의 뒷면에 오브젝트를 설치하여 노광했다. 기록재료는 빔 스프린터의 역할을 했다. 하이브리드 기록 시스템은 네 가지 홀로그램을 독립적이고 유기적으로 제작하기에 적합하였다. 한 대의 레이저로 구성 된 하이브리드 기록 시스템 에 의해 안정적으로 프레넬 홀로그램, 리프만 홀로그램, 데니슈크 홀로그램을 제작하기에 적합하였다. 하이브리드 기 록 시스템을 도입하여 투과와 반사 타입의 홀로그램을 제작 할 수 있었고 홀로그램 카메라로 실현 가능하다는 것을 확인하였다.
        4,000원
        5.
        2016.04 구독 인증기관·개인회원 무료
        Biological control has been tried for integrated pest management. It is often comparable, safe, and environment-friendly, making itself an alternative for chemical agents. Filamentous microorganisms, i.e., fungi and streptomystes, produce many kinds of useful metabolites, and some of them have been developed as a biocontrol agent. However, they still have a long way because of the concern of manufacturing cost. Therefore, process development was intensively studied to meet cost-effectiveness. Operating conditions of bioreactor, e.g., agitation and aeration, had an effect on biological and physiological responses such as mycelial morphology, oxygen and nutrient transfer. Understanding relationship between operating parameters and microbial responses in terms of growth, substrate and oxygen consumption, and production yield was critical for process development. This study dedicated to build strategies for mass production of biological control agent using aerobic filamentous microorganisms.
        7.
        2015.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study was to parallel circuit training and circuit training with sonic systemic mechanism was to compare the differences in pulmonary function and chest expansion in adult men. This study was performed on 20 subjects. 20 subjects were divided into two groups; Circuit training group(n=10), Circuit training with sonic systemic mechanism(n=10). Both of the group performed the exercise 3 times a week for 5 weeks. The data was analyzed by the Repeated t-test for comparing before, during and after changes of factors in each group and the Independent t-test for comparing the between groups. The result are as follows. Circuit training group was statistically significant difference FVC, FEV1/FVC(p<.05), Circuit training with sonic systemic mechanism group was statistically significant difference PEF, VC in pulmonary function(p<.05). Circuit training group was statistically significant difference FEV1/FVC of between the two group in pulmonary function(p<.05). Circuit training group and circuit training with sonic systemic mechanism group was statistically significant difference in chest expansion(p<0.05) and there was no statistically significant difference of between the two group in chest expansion(p>.05).
        4,000원
        8.
        2015.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study was to investigate the effects of vibration on Golgi tendon organ(GTO) and Hold-Relax of PNF in muscular activity and gait factors on Delayed Onset Muscle Soreness(DOMS). This study was conducted on 20 subjects. they were divided into two groups; Hold-Relax of PNF(n=10), Vibration on GTO(n=10). Both of the group was performed interventions 1 times a day for 3 days. The data was analyzed by the repeated-ANOVA for comparing before, after 24h and after 48h changes of factors in each group and the Independent t-test for comparing the between groups. The results are as follows. There was statistically significant difference of before, after 24h and after 48h vibration on GTO group and Hold-Relax of PNF group in muscular activity and gait factors on DOMS.(p<0.05). There was no statistically significant difference of between vibration on GTO group, but there was statistically significant difference Hold-Relax of PNF group in EMG, step width, step length, stride length(p>0.05). As a results of this study, Hold-Relax of PNF group are effective in improving muscular activity and gait factors
        4,000원
        9.
        2014.03 구독 인증기관 무료, 개인회원 유료
        볼륨 타입의 컬러 홀로그래픽 스테레오그램을 디지털 방식으로 제작하기 위해 광학 시스템에 대해 연구하였다. 필름으로 노광하는 기존의 과정과 비교해 간략화하고 자동화된 볼륨 컬러 홀로그램의 제작을 위해 DLP 프로젝트를 도입하여 광학시스템을 디자인했다. DLP 프로젝트를 이용하여 시차 이미지를 확산판에 투영하고 물체광을 생성시켰다. 참조광은 유제면의 반대 방향으로 입사시켜 간섭무늬를 만들었다. 시차이미지는 색분해 하여 Red, Green, Blue의 색 성분으로 만들어 공간에서 분할하는 기록 방법으로 컬러 홀로그래픽 스테레오그램을 제작했다. 660nm, 532nm, 457nm의 파장을 지닌 세 대의 레이저 광원을 사용해 컬러 홀로그래픽 스테레오그램을 2단계로 나누어 기록했다. 마스터 홀로그램에서 공액 재생법으로 실상을 재생시켜 리프만 홀로그램 타입으로 제작하였다. 제안하는 디지털 방식의 광학 시스템으로 볼륨 타입의 컬러 홀로그래픽 스테레오그램을 실현 가능하다는 것을 확인하였다.
        4,000원
        10.
        2013.03 구독 인증기관 무료, 개인회원 유료
        원스텝 방식으로 투과 타입의 홀로그램을 제작할 수 있는 디지털 광학 시스템에 대해 연구 하였다. 원스텝 방식의 광학 시스템에서는 LCos panel과 DLP Project를 사용해 홀로그램 영상을 촬영하였다. 평면 타입의 홀로그래픽 스테레오그램을 합성 할 때는 반사형의 LCos panel를 사용하여 요소 홀로그램을 제작했다. 시차 이미지를 LCos panel에 순차적으로 표시하면서 기록 재료를 이동시켜 홀로그래픽 스테레오그램으로 합성하였다. 레인보우 홀로그램과 이미지 홀로그램을 노광할 때는 DLP Project를 사용하였다. 레인보우 홀로그램의 광학 시스템에서는 DLP Project와 결상 렌즈 사이에 슬릿을 설치하고 슬릿의 실상을 기록재료 뒤쪽에 재생되도록 구성하였다. DLP Project 별도의 구동 드라이버가 필요 없었고 진동에도 강해 안정적으로 노광하기에 적합하였다. 디지털 방식을 광학 시스템에 도입하여 원스텝으로 투과 타입의 얇은 홀로그램을 실현 가능하는 것을 확인하였다.
        4,000원
        11.
        2012.10 구독 인증기관·개인회원 무료
        Varieties of Bacillus thuringiensis (Bt) crystal proteins, Cry proteins, have so far been found as one of the most successful biological control agents which are safe to natural environments for a long time. Recently, cry genes encoding these Cry proteins have been widely applied for construction of transgenic crops resistant to pest insects. In this study, through the 3D structure prediction and accompanying mutagenesis study for the Mod-Cry1Ac, 7 and 16 amino acid residues from domain I and II, respectively, responsible for its insecticidal activity against larvae of Spodoptera exigua and Ostrinia furnacalis were identified. To construct novel cry genes with improved insecticidal activity, we randomly mutated these 23 amino acid sequences by in vitro muti site-directed mutagenesis, resulting in totally 24 mutant cry genes. For further characterization, these mutant cry genes were expressed as a fusion protein with polyhedrin using baculovirus expression system. SDS-PAGE analysis of the recombinant polyhedra revealed that expressed Cry proteins was occluded into polyhedra and activated stably to 65 kDa by trypsin. In the further study, we plan to investigate their insecticidal activity against Plutella xylostella, S. exigua and O. furnacalis larvae.
        12.
        2012.03 구독 인증기관 무료, 개인회원 유료
        입체 영상을 관찰할 수 있는 홀로그램 지향성 스크린을 제작하기 위해 In­line 홀로그램 기록 시스템에 대해 연구하였다. 홀로그램 지향성 스크린을 이용해 입체 영상을 관찰할 때 시역의 위치에서 이상적인 지향성의 산란광과 0차광을 완화하는 방법을 제시했다. 이상적인 지향성의 산란광을 설계하기 위해 기록재료에서 확산판을 바라볼 때 생성되는 각도의 크기를 변화시키면서 노광했다. 0차광을 완화하기 위해서는 확산판에 Apodization 처리 한 뒤에 홀로그램을 제작했다. 시역을 재생할 때는 릴레이 렌즈 시스템을 설계하여 0차광이 완화되도록 했다. In­line 홀로그램에 의한 기록시스템에 있어 기록재료에서부터 확산판을 바라볼 때 생성되는 각도가 작은 상태로 제작한 홀로그램 일수록 지향성의 산란광 특성이 양호하다는 것을 확인하였다. 릴레이 렌즈로 구성된 재생시스템으로 회절상을 재생했을 때 스펙클 패턴은 발생하지 않았고 0차광이 완화되는 것을 확인하였다.
        4,000원
        13.
        2011.03 구독 인증기관 무료, 개인회원 유료
        홀로그래피 기술을 응용해서 입체 영화를 만들기 위해 프레넬 방식의 슬릿 홀로그램과 영사시스템에 대해 연구하였다. 슬릿 홀로그램은 한 장의 기록 재료에 영역을 나누어 중복되지 않게 오브젝트를 바꾸어가면서 기록했다. 슬릿 홀로그램은 세로로 긴 직사각형의 모양으로 연속적으로 만들었으며 영화필름과 비교하면 프레임과 같은 역할을 한다. 영사시스템은 레이저 광원, 개구, 슬릿 홀로그램, 볼록렌즈, 프레넬렌즈, 프로젝션 렌즈로 구성했다. 슬릿 홀로그램으로부터 허상을 먼저 재생하고 실상으로 전환하기 위해 슬릿 홀로그램의 앞과 뒤에 개구와 볼록 렌즈를 설치해 3층 구조로 만들었다. 홀로그램으로부터 입체 영상을 재생하는 방법으로는 허상을 먼저 재생한 뒤 볼록 렌즈에 의해 실상으로 전환했다. 슬릿 홀로그램을 기록할 때 오브젝트와 홀로그램까지의 거리를 조정함으로써 대화면의 입체 영상을 재생할 수 있다. 영사시스템에서는 투영 렌즈와 프레낼 렌즈의 초점거리에 변화를 줌으로서 대화면의 영상으로 투영할 수 있었다. 슬릿 홀로그램과 영사시스템에 의해 대형화면으로 스크린에 투영할 수 있었고 입체영화로 실현 가능하다는 것을 확인하였다.
        4,000원
        14.
        2009.12 구독 인증기관 무료, 개인회원 유료
        The purpose of this study is to evaluate the effects of alcohol or cigarette smoking on seminal parameters in a large group of mice model. Nine groups (n=20/group) of mice were treated intensive noxious materials that abdominal injection of 21% (v/v) of ethanol, cigarette smoke (10, 20, 30 minutes/day), and combination of ethanol and 30 minutes of smoking. In addition, vitamin C and selenium were also treated to mice exposed to combination of alcohol and smoking to identify the recovering effect. Sperm viability and motility were significantly decreased in either alcohol consumption or smoking exposed group, and combination of both materials have additive detrimental effects on seminal parameters. Mice groups that exposed to alcohol and smoking showed statistically significant decrease in motility and increase of static spermatozoa. Moreover, combination of both treatments showed cumulative effect in increase of static spermatozoa. Treatments of either vitamin C or selenium dramatically recovered detrimental effects of alcohol and smoking on seminal quality, although combination of both antioxidant molecules did not show any additive effect. In conclusion, detrimental effects of alcohol and cigarette consumption on sperm quality and motility were identified in mice model, and these detrimental effects can be compensated to uptake of anti‐oxidant molecules.
        4,000원
        19.
        2016.10 서비스 종료(열람 제한)
        Background : This text was conducted to investigate the fruit type of Camellia japonica in ko rea. Methods and Results : In fruit of Camellia japonica, For fruit length of 24.1~33.0mm was 6 3.7%, and width of 30.1~39.0mm was 60.2%, and weight of 10.1~25.0g was 62.1%. Fruit was near in circle shape, because length/width ratio 0.71~1.00 indicate 79.6%. Seed number of per fruit by weight was also tends to be which is much as fruit is heavy. In correlation b etween each of fruit character, Fruit width showed a positive correlation with the fruit length, fruit weight, seed number. Also, for seed number, the correlation coefficient is the fruit weigh t (0.643**), fruit width (0.640**) was significantly more than the length (0.532**). Conclusions : Camellia japonica was very various size, shape of fruit and seed number of per fruit.
        20.
        2014.09 서비스 종료(열람 제한)
        The transcription factor, early growth response protein 1 (EGR1), act as immediate early response genes to control various cellular and reproductive events. Egr1-deficient female mice show infertility by anovulation resulting from luteinizing hormone-β (LH-β) subunit deficiency. While ovulation, fertilization and embryo development normally occur in Egr1-deficient mice treated with a superovulation regime to rescue LH deficiency, embryo implantation was completely failed. The morphology and ultrastructure of uterine tissues were observed by light and transmission electron microscopy during the peri-implantation period in Egr1-deficient mice. To examine alterations in cellular organelles, the uterine horns were fixed with 2.5% glutaraldehyde and postfixed with 1% osmium tetroxide in PBS. After dehydration and infiltration, the samples were embedded in Epon 812. Semi-thin sections 0.5 μm thick were cut with an ultramicrotome and stained with toluidine blue for light microscopy. Thin sections were cut with a diamond knife of the ultramicrotome and placed on copper grids. The sections were double stained and examined under a transmission electron microscope. The height of luminal epithelial cells was decreased and the polarity was poorly differentiated in the Egr1-deficient comparing to the wild mice. The abundant mucinous materials were observed in the surface of luminal epithelial cells of the Egr1-deficient. It was confirmed the microarray and real time qPCR data. The luminal epithelial cells of wild mice had many dense lipophilic granules and healthy mitochondria, but not in the Egr1-deficient. It may related to production and secretion of steroid hormones and prostaglandins in the luminal epithelial cells for successful implantation. These results show that Egr1 is a critical transcription factor to fine-tune subcellular morphological and functional changes for the receptive phase of peri-implantation period of uterine tissue in mice.
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