Semen can be divided into two parts. One is cellular part which contains sperms the other is liquid part which is called by seminal plasma. The seminal plasma is a nutritive and protective medium for the sperms. Fructose, which is major energy source, is supplied to sperms swim to female oocyte. Alkalic property protects sperms from hostile environment of female reproductive organ. Also, seminal plasma induces tolerance to preexisted immune cells, and changes intra‐uterine environment to better conditions for fertilized embryos to implant. However, the effects of seminal plasma in in vitro culture of fertilized embryos are unclear. Second fraction of fresh semen was obtained from a normal farm pig. The semen was centrifuged to remove sperms, and then supernatant was filtrated. The filtered seminal plasma was stored in — 30℃. In this study, electrically activated and chemically activated porcine embryos were employed to investigate the developmental rate after 2 hours treatment of none, 0.1%, 0.5%, and 1% seminal plasma in culture media by two days of activation. Both electrically and chemically activated embryos, cleavage rate and cell numbers of blastocysts were not significant difference within four groups. Blastocyst formation rate of electrically activated embryos also did not show significant difference within any groups. However 0.1% seminal plasma treatment group showed significantly increase of blastocyst formation rate in chemically activated group (None; 24.8%, 0.1%; 31.7%, 0.5%; 19.4, and 1%; 16.5%, respectively. p<0.05).

This study focused on the buildings, wharves, and parks of the coastal space of Chongqing based on the ten-year construction draft plan of the alternate capital. From an investigation of these three components according to their type, structure, terrain, function, spatial layer, and so on, the following conclusions were drawn: The coastal space of Chongqing is composed of three different layers. The first and second layers consisted of the two-river intersection basin with the transportation and commemorative space at the center, the Yangtze River basin with the commercial space at the center, and the Jialing River with the military and recreational space at the center. The third-layer is centered around the commemorative space. The coastal space of Chongqing manifested the limitations of the times, its traditionality, and an integrated development process.

On the basis of conducting a comprehensive investigation to agricultural sightseeing garden landscape resources, we established a evaluation index system of agricultural sightseeing garden landscape resources. From the perspective of experts, comparing the importance of all levels indicators, we obtained the weights of landscape resources evaluation index through using the Analytic Hierarchy Process. The result showed that the descending order of the weights of influence of agricultural sightseeing garden nineteen evaluation index is: reach ability (0.128), safety (0.083), location (0.078), participatory (0.076), cultural value (0.058), ecological conditions (0.057), scenic beauty (0.0505), environmental quality (0.051), featured properties (0.0501), environmental tolerance (0.048), reputation (0.047), environmental capacity (0.045), humanize (0.041), spots configuration (0.034), applicable travel period (0.033), scientific value (0.032), art value (0.031), holistic (0.03), suitability (0.027), it can provide a framework and basis for the planning, management, protection and exploitation of agricultural sightseeing garden landscape resources.

This paper is to study the tendency of the development of the urban comprehensive park in the modern history of China, and take the urban comprehensive park of Wuhan as an example to study the local research through the literature.. In 1928, Wuhan Urban Comprehensive Park starting stage characteristics to the ideological education of the government as the center, in 1977 the policy of reform and opening to the outside world to entertainment center, ecology design point of view, the introduction of open space and urban plaza, to human centered design, application of science and technology. 2015 in Wuhan Urban Garden Expo theme can be seen in green life as the goal, through the design of urban comprehensive park ecology and human centered design, the application of science and technology development.