Understanding of a carbon storage in a regional scale ecosystem is a very important data for predicting change of global carbon cycle. Therefore, the real data collected in the various ecosystems are a very useful for enhancing accuracy of model prediction. We tried to estimate total accumulated ecosystem carbon in Deogyusan National Park (DNP) with naturally well preserved ecosystem. In DNP, vegetations were classified to four main communities with Quercus mongolica community (12,636.9 ha, 54.8%), Quercus variabilis community (2,987.0 ha, 13.0%), Pinus densiflora community (5,758.0 ha, 25.0%), and Quercus serrata community (402.9 ha,1.7%). Biomass and soil carbons were estimated by the biomass allometric equations based on the DBH and carbon contents of litter and soil (0~30 cm) layers collected in 3 plots (30 cm×30 cm) in each community. The biomass and soil carbons were shown as high value as 1,759,000 tC and 7,776,000 tC, respectively, in Quercus mongolia community in DNP area. In Quercus mongolica, Quercus variabilis, Quercus serrata, Pinus densiflora communities, the accumulated ecosystem carbon were shown 9,536,000 tC, 1,405,000 tC, 147,000 tC, 346,000 tC, respectively. Also, the total ecosystem carbon was estimated with 11,434,000 tC in DNP.