The blackish cicada, Cryptotympana atrata Fabricius, 1775 (Hemiptera: Cicadidae) was originally distributed mainly in the southernmost remote island, Jeju and rarely throughout low lands in South Korea, but has been explosively increased at the urban areas, where annual temperature is higher. In this study, we sequenced a partial mitochondrial COI from a total of 171 individuals collected throughout 12 localities in South Korea. The haplotype found with the highest frequency in Jeju island shares only with two inland localities in southern region with a low frequency, whereas the haplotype found with the highest frequency throughout inland localities was not found in Jeju island. These results showed that Jeju population, southern region, and other inland populations form somewhat different genetic groups.

The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

Tropilaelaps mercedesae Anderson and Morgan, 2007 (Acari: Laelapidae) is a serious ectoparasite of the brood of several honey bee species. Among the four recognized species of Tropilaelaps, Korean population was renamed as T. mercedesae from T. clareae on the basis of morphological evidences and genetic data. In this study, we report the complete mitochondrial genome (mitogenome) sequence of T. mercedesae. The 15,119-bp long mitogenome has an identical gene arrangement to that of Chinese sample reported previously. Comparison of two geographic samples showed COII, ND5, ND4, ND6, CytB, and ND1 to have higher number of variable sites than COI, which is often used for population-level study, suggesting these genes to have potential usefulness for population genetic study. The mitogenome sequence of T. mercedesae from Korea could be useful for species identification for geographic samples, trace of the origin of local populations, and illustration of evolutionary distinction among Tropilaelaps species either using part of or whole genome.

Vespa binghami (Hymenoptera: Vespidae) is one of the 10 species in the genus Vespa distributed in South Korea and is the only nocturnal wasp. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species using the Sanger method and using Vespa-specific 30 primer sets. The length of V. binghami was 15,957 bp and the total A/T content was 80.6%. The A+T-rich region of V. binghami was 152 bp, and other Vespa species ranged from 39 bp (V. velutina) to 2,230 bp (V. v. auraria). Compared to the majority of insects, the gene arrangement of V. binghami had differences as followings: trnY-trnI-trnM-trnQ, trnN-trnE-trnS1-trnF, and trnS2-trnL1. However, all species in the genus Vespa registered in GenBank to date were composed of the same arrangement. Phylogenetic analysis using 13 PCGs and 2 rRNA genes showed the sister relationship between V. binghami and V. orientalis with the higher nodal supports.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of the domesticated silkworm B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We sequenced 100 mitochondrial genomes of B. mandarina collected from South Korea and Japan and these were combined with public data. Phylogenetic and population genetic analyses showed that all individuals collected in South Korea, except one individual from Inje, formed a strong group together with northern China and some individuals of southern China. This group was placed as the sister group to B. mori strains, suggesting that this group could have been served as an immediate progenitor for B. mori. For further robust inference more analysis is underway.

The pear pest, Cacopsylla jukyungi (Hemiptera: Psyllidae), is one of the most damaging insect to commercial pears in South Korea. In this study, we developed eight microsatellite markers specific to C. jukyungi and genotyped 132 individuals collected from 11 localities throughout South Korea. Populations showed lower observed heterozygosity than expected heterozygosity and slightly or highly positive values of inbreeding coefficients, suggesting that C. jukyungi is subjected to inbreeding. The nationwide expansion of pear orchards and the replacement with a popular new cultivar during the last 50 years, which may have accompanied the spread of C. jukyungi-bearing pear grafts and scions, are likely sources of such facilitated dispersal. Thus, a management strategy against unintended anthropogenic dispersal of the pear psyllid will be required for better control of C. jukyungi.

Ceriagrion nipponicum (Odonata: Coenagrionidae) is listed as climate-sensitive indicator species in Korea and now expands its range northward. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species collected from South Korea for comparatively analysis in damselflies and to detect the genes suitable for subsequent population genetic study. Comparison of the mitogenomes from two geographic samples of C. nipponicum showed the highest variation in ND4 and ND1, whereas no variation was detected in COI, warranting usefulness of the two genes for subsequent population-level study. Phylogenetic analysis using 13 PCGs and 2 rRNAs in Zygoptera showed non-monophyletic Coenagrionidae, forming two groups.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.

In Korea, both Sympetrum depressiusculum Sélys, 1841 (Odonata: Libellulidae) and Sympetrum frequens Sélys, 1883 are recorded. However, the identity of Korean populations and the validity of listing the two species have not yet been settled. In this study, we collected 74 individuals from Kroea, Russia, the Netherlands, and Japan. These were sequenced for COI, 16S rRNA, and ITS region. Major morphological characters and phylogenetic, network, and structure analyses all consistently suggest that Korean populations form a single species. Consequently, it could be valid to treat Korean populations as one species, S. depressiusculum, by applying the senior name.