Background: This study investigated quadriceps femoris muscle activity by comparing concentric and eccentric contractions with self-selective squat speed. Objectives: Study participants agreed in advance and included 30 male college students in their 20s without musculoskeletal system disease. Design: A randomized controlled trial. Methods: Participants who participated in this study agreed in advance and recruited 30 male college students in their 20s who had no disease with musculoskeletal system to conduct this study. When squatting at a self-selected speed, the subjects were evaluated using one-way ANOVA to compare concentric and eccentric contraction muscle activity. Results: There were significant differences between the rectus femoris, vastus medialis, and vastus lateralis regarding concentric contractions.contractions. There was a significant difference in vastus medialis but no significant difference between rectus femoris and vastus lateralis for eccentric contractions. Conclusion: During concentric contraction, all muscles and eccentric contractions indicated significant improvement in the vastus medialis. Therefore, to minimize knee joint injury and maximize efficient muscle activity, self-selected slow and moderate speeds based on self-selection speed when performing the sit-down motion during a squat and moderate speed with fast motions when performing the standing motion should be considered as high muscle activities.