기존에는 생산되는 키틴과 키토산의 대부분이 게, 새우등 갑각류 껍질에서 유래하였다. 하지만 어업에 의존하 는 기존 갑각류 비해 친환경적이며 품질 유지에 이점을 가지는 곤충으로부터 유래한 키틴이 최근 주목 받기 시작 하며 연구가 활발해지고 있다. 이에 키토산이 남조류의 응집을 통해 녹조 제거 효과를 가지며 기존에 녹조를 억제하기 위해 널리 사용되던 살조제들이 독성을 띠어 환경에 악영향을 미치는 문제를 해결할 수 있다는 연구를 참고하여 매미 탈피각으로부터 추출한 키토산을 녹조 방제에 활용해 보고자 하였다. 매미 탈피각으로부터 키토 산을 추출하고 대표적인 녹조 원인종인 Microcystis aeruginosa 배양 후 추출한 키토산을 처리하여 녹조의 응집 효과를 관찰하였다. 본 연구에서 새로운 키토산 추출 원으로서 매미 탈피각의 가능성을 제시하였으며 이를 녹조 방제에 활용함으로써 버려지는 자원인 매미 탈피각의 활용 방안을 제시하였다.

다른 장르의 게임에 비해 포커는 게이머의 심리적 요소가 많은 영향을 끼친다. 본 논문에서는 CNN과 SVM을 기반으로 온라인 포커 게임에 게이머와 아바타 간의 감성연결을 실현하기 위한 새로운 감성 인식방법을 제안한다. CNN모델을 이용하여 원래 얼굴 이미지의 특징을 추출하고, 다중 클래스 SVM분류기를 사용하여 목표 이미지를 인식하고 분류한다. FER-2013데이터베이스에서 이 방법은 감성인식률 68.79 %를 달성하였다. 기존의 다른 감성 인식 모델과 비교하면, 이 모델은 뚜렷한 장점을 보일 수 있다. 본 게임은 Socket 통신방식을 통해 감성인식결과를 Seven Poker로 전송하여 아바타가 게이머와 같은 감성을 표현하도록 설계하였다. 온라인 포커 게임에 감성연결 기술을 이용하면 게임과 인간의 상호작용이 향상될 뿐 아니라 게이머가 상대방의 심리적인 활동을 효과적으로 분석할 수 있다. 감성연결 기술은 게임에서 게이머들에게 새로운 게임 경험을 제공할 수 있는 기술이라고 생각된다.

Honeybee (Apis mellifera) egg-yolk protein vitellogenin (Vg) plays roles in immunity, antioxidation, and life span beyond reproduction, but it also acts as an allergen Api m 12 in venom. Here we established antimicrobial and antioxidant roles of honeybee Vg in the body and venom. Using the cDNA encoding Vg identified from Asiatic honeybee (A. cerana) workers, recombinant A. cerana Vg (AcVg) protein of approximately 180 kDa was produced in baculovirus-infected insect cells. In A. cerana worker bees, AcVg was expressed in the fat body and venom gland and was present in the secreted venom. AcVg induced structural damage in microbial cell walls via binding to microbial surfaces and exhibited antimicrobial activity against bacteria and fungi. AcVg protected mammalian and insect cells against oxidative damage through direct shielding of cell membranes. Interestingly, AcVg exhibited DNA protection activity against reactive oxygen species (ROS). Furthermore, the transcript level of AcVg was upregulated in the fat body, but not in the venom gland, of worker bees with antimicrobial peptides and antioxidant enzymes in response to microbial infection and oxidative stress. Our data indicate that AcVg is involved in innate immunity upon infection and in a defense system against ROS, supporting a crucial role of honeybee Vg as an antimicrobial and antioxidant agent in the body and venom.

The honeybee inhibitor cysteine knot (ICK) peptide acts as an antifungal peptide and insecticidal venom toxin. However, the ICK peptide from bumblebees has not been characterized. Here, we report the molecular cloning and antifungal activity of a bumblebee (Bombus ignitus) ICK peptide (BiICK). We identified a BiICK that contains an ICK fold. The BiICK was expressed in the epidermis, fat body, and venom gland of B. ignitus worker bees. A 6.7-kDa recombinant BiICK peptide was expressed in baculovirus-infected insect cells. Recombinant BiICK peptides directly bound to Beauveria bassiana, Ascosphaera apis, and Fusarium graminearum, but they did not bind to Escherichia coli, Paenibacillus larvae, or Bacillus thuringiensis. Consistent with this finding, BiICK exhibited antifungal activity against fungi. These results demonstrate that BiICK acts as an antifungal peptide.

MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.

수평 배향 액정 모드는 양의 유전율 이방성과 음의 유전융 이방성 액정을 사용한 프린지 필드 스위칭과 양의 유전율 이방성 액정을 사용한 인플래인 스위칭 모드가 대표적이다 . 이 대표적인 세 구동 방식의 화질 특성을 비교하기 위하여 각각의 최척화된 위상지연 값 조건하에서 밝기, 명암대비율과 색 특성을 조사하였다. 그 결과 양액정과 음액정을 사용한 프린지 필드 스위칭 모드가 밝기와 명암대비율 면에 있어서 인플래인 스위칭 모드보다 우수한 특성을 보인다. 또한 양액정을 사용한 프린지 필드 스위 칭 모드는 시야각 방향에서 적은 색 변이 특성을 보인다.

Background : This research was conducted to investigate the effect of organic compost treatment on growth and yield of organically grown ginseng seedlings(Panax ginseng C.A. Mayer) in growing medium. Methods and Results : The test cultivars was used a native species of Panax ginseng C.A. Mayer that most commonly cultivated in Korea. By seeding interval(3×3cm), seeds of Panax ginseng C.A. Mayer were sowed on March 29, 2016 . Bed size was 85 (W) × 1800 (L) × 30 (H) cm and shading material of plastic house was used blue-white vinyl. Growing medium composition(V/V) were mixed in a ratio of peat moss 60%, perlite 30%, vermiculite 10%. The mixing ratio of Organic compost was livestock manure compost (2%, V/V), mixed oil cake(2%, V/V), poultry manure compost (2%, V/V) and was applied to growing medium basal application. The emergence rate of ginseng seedling was contorl 95.7%, livestock manure compost 97.0%, press cake 92.3%, poultry manure compost 94.2%. Shoot growth characteristics of ginseng seedlings did not show a significant difference in plant height, stem length, stem diameter but shoot fresh weight was higher tendency in livestock manure compost(0.34 g/plant) in comparison to control(0.26 g/plant), mixed oil cake(0.29 g/plant), poultry manure compost(0.27 g/plant). Similarly, root fresh weight was higher tendency in livestock manure compost(0.52 g/plant) in comparison to control(0.33 g/plant), mixed oil cake(0.47 g/plant), poultry manure compost(0.43 g/plant). Root length was in control 10.2cm, livestock manure compost 11.8cm, mixed oil cake 8.9cm, poultry manure compost 8.6cm. As mentioned above, the reason that root length is shorter in mixed oil cake, poultry manure compost is thought to be due to the influence of gas injury. Conclusion : Growth characteristics of ginseng seedling showed a good growth trend in the organic compost treatment groups , especially livestock manure treatment root fresh weight increased 57% compared to control group. However, early defoliation phenomenon occured at mixed oil cake, poultry manure compost in late June. This is thought to have occurred by the disturbance of gas such as ammonia gas, nitrous acid gas. Therefore, if you use mixed oil cake, poultry manure compost to cultivate ginseng seedling, it is expected to pay attention to the damage caused by the gas disturbance.

Compared to wide ranges of genetic variation of natural populations, very limited Miscanthus cultivar has been released. This study was the first report on the development of Miscanthus cultivar by means of radiation breeding. Seeds of M. sinensis were initially exposed to gamma rays of 250 Gy for 24 h, generated from a 60Co gamma-irradiator. The irradiated seeds were sown and then the highly tiller-producing mutants were selected for this study. Biomass-related parameters including tiller number, plant height, stem diameter, and leaf number were measured. Ploidy level and internal transcribed spacer (ITS) were investigated to characterize the mutants compared to wild type (WT) Miscanthus. Plant height and tiller number were negatively related, where multi-tillering mutants were relatively short after 4 month growth. However stem diameter and leaf number were greater in mutants. All the materials used in this study were diploid, implying that the mutants with greater tiller numbers and stem diameter were not likely related to polyploidization. Based on the sequence of ITS regions, the mutants demonstrated base changes from the gamma irradiation where G+C content (%) was decreased in the ITS1, but increased in ITS2 when compared to WT sequence. ITS2 region was more variable than in ITS1 in the mutants, which collectively allows identification of the mutants from WT. Those mutants having enhanced tillers and allelic variations might be used as breeding materials for enhanced biomass-producing Miscanthus cultivars.

Rye (Secale cereale L.) chromatins have been used to introduce agronomically important traits into wheat (Triticum aestivum L.). Wheat-rye translocations in the form of 1RS.1AL, 1RS.1BL, 2BS.2RL have been developed for an important genetic source of disease and pest resistance. The long arm of rye chromosome 2 (2RL) has valuable genes that confer resistance to pests such as biotype L of Hessian fly, powdery mildew, leaf and stem rust. Here, we report the generation and analysis of expressed sequence tags from Hessian fly infested wheat-rye translocation. RNAs were isolated from young seedlings infested by Hessian fly. cDNA library was constructed using Clontech cDNA library construction kit. Random sequencing of candidate clones were performed. The EST clones might be useful to clone target gene sequences and would provide clues on molecular interaction between wheat and Hessian fly.

barley grain and malt is highly related to beer quality, especially hordein is known to be a more significant factor in malting process than albumin. In this study, we proposed selection criteria for high quality malting barley with aid of grain and malt quality parameter scores and storage protein subunit profile informations. Albumin and hordein were extracted and denatured protein subunits were evaluated with malt and grain quality parameters. Total 13 local adaptability test (LAT) lines were planted in four locations (Naju, Iksan, Jeju, and Jinju) and evaluated for malt and beer making qualities. Seventeen germplasms (world collections for high or low seed storage protein content) were also evaluated for biochemical genetic marker. Denatured seed storage protein subunits of albumin and hordein of all tested lines and germplasms were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis. Subunits fractionated within specific molecular weight ranges (97.4-31.0, 66.2-31.0, and 45.0-31.0 kDa) were highly correlated with agricultural characteristics. Several LAT lines showing good performance in agricultural characteristics were clustered in dendrogram constructed by biochemical-genetic assay using XLSTAT. Specific band pattern showed in good performance LAT lines were also observed in some germplasms of world collection having low protein contents which are known to have superior quality in malting. The results would provide selection criteria for high quality malting barley in the malting barley breeding program.

UNUSUAL FLORAL ORGAN (UFO), a novel gene, is involved in controlling flowering initiation and development. In Arabidopsis, UFO is required for floral organ identity in the second and third whorls. However, the mode of expression and function of TaUFO have not been studied yet. The cDNA sequence of TaUFO is comprised of 1344 bp open reading frame which encodes 50.82 KDa polypeptide consisting amino acid residues. F-box protein, the components of TaUFO, plays an important regulatory role in a wide diversity of developmental and physiological responses. In almost all F box proteins, the N terminus of the protein contains the F-box motif, and the rest of the protein contains the protein-protein interaction domains required for target protein binding. In order to elucidate the function of the TaUFO, various phytohormones and abiotic stresses were applied on young seedlings (14 day after germination) and its transcripts were evaluated. TaUFO:GFP fusion construct was transformed into onion epidermal cells by particle bombardment to elucidate the subcellular localization of the TaUFO protein. The function of the F-box protein is to interact with target proteins. With the use of a yeast two-hybrid screen to isolate proteins interacting with the TaUFO (F box protein), we identified potential TaUFO interactive protein in wheat spikelet library.