Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, many kinds of compounds have been evaluated for their nematicidal activity against PWN with different bioassay system according to researchers. Therefore, it was hard to directly compare their activity. To establish standard procedure for bioassay, nematicidal activity of abamectin was tested and compared in different conditions. The tested conditions are following: 1) nematode density: 100 and 1000 nematodes in 0.1 and 1 mL, 2) amount of reagent: 0.1, 1, 10, 100 uL of abamectin solution which same amount of abamectin was dissolved in. We will report the results of tested nematicidal activity according to conditions.