Swine atrophic rhinitis is a respiratory disease that causes nasal turbinate loss and septal deformation due to Bordetella bronchiseptica and Pasteurella multocida. Turbinate loss facilitates pathogens to infect lungs, which leads to various respiratory diseases and productivity reduction. In this study, descriptive analysis was implemented for atrophic rhinitis and pneumonia. From 6 pig farms shipped to slaughterhouses in Chungbuk province, 20 heads and 20 lungs were collected by each farm from March 2020 to September 2020. Their atrophic rhinitis lesions and lung lesions were scored and blood samples were also collected to test the seroprevalence of several respiratory diseases. Pasteurella multocida from nasal swab was cultured and antibiotic resistance tests were performed. Correlation between atrophic rhinitis scores and lung lesion scores was not found. Abdominal nasal lesions were more severe than dorsal lesions. Differences in lung lesion scores were relatively small between lobes. The score of pneumonia was higher in castrated pigs than in female pigs. There was no relationship between lesion score and seroprevalence of respiratory diseases. Antibiotic resistance levels for Pasteurella multocida differed by farm, and several antibiotics were not effective. The results of this study imply that antimicrobial susceptibility tests are highly recommended before administration.

Fowl adenovirus serotype-4 (FAdV-4) infection, also known as hydropericardium-hepatitis syndrome (HHS), is one of the most prevalent diseases in the Korean poultry industry. Therefore, an effective vaccination against FAdV-4 should be developed for prevention of HHS infection. However, a standard animal model with significant pathologic characteristics has not been established for evaluation of HHS vaccine efficacy. In this study, we comparatively evaluated FAdV-4 pathogenicity from specific- pathogen-free (SPF) chickens using FAdV-4 strain (ADL091024) isolated in laying chickens in Korea according to chicken age, passage number and virus titers. In brief, FAdV-4 passaged four times in yolk sac of SPF eggs with the highest viral titers could induce the most severe clinical signs in 1-day-old chickens, similar to natural HHS outbreaks. Furthermore, the efficacy of a newly developed attenuated live FAdV-4 vaccine candidate was successfully determined using the established 1-day-old SPF chickens. There was no significant pathogenicity based on the criteria such as mortality, body weight, gross lesions, histological lesions and virus detection rates in the vaccination and non-challenge control groups. However, the challenge group without vaccination showed significant pathogenicity including hepatic necrosis, histological lesion scores in the liver and heart, virus replication and decreased body weight gain. In conclusion, the chicken challenge model established in this report was proposed for consistent and reliable evaluation of FAdV- 4 vaccine efficacy.

본 연구는 대학생을 대상으로 하여 컴포트 푸드(comfort food)의 구성요인과 종류, 그리고 희노애락의 4개 정 서에 따른 컴포트 푸드의 섭취에 성차가 존재하는가를 확인하고자 하였다. 이를 위해 대학생 425명을 연구 참가 자로 선정하여 설문조사를 실시하였다. 그 결과 첫째, 남학생과 여학생 모두에서 따뜻함과 위안을 주는 음식, 즐 거운 추억을 떠올리게 하는 음식, 맛있는 음식을 컴포트 푸드의 개념으로 가장 높게 지각하고 있었으며, 음식 외 적 요소에서 부담 없는 음식, 편리한 음식을 컴포트 푸드의 개념으로 가장 낮게 지각하고 있었다. 그러나 건강을 고려한 음식에서는 여자가 남자보다 컴포트 푸드로 더 적합하게 생각하는 것으로 나타났다. 둘째, 컴포트 푸드의 종류로 남자는 고기류, 찌개 및 백반, 술을 보고하였고, 여자는 찌개 및 백반, 과일 및 야채, 고기류를 보고하였 다. 남자가 음식종류를 선호하고 여자가 스낵 종류를 선호한다는 가설을 지지되지 않았다. 셋째, 제시된 4개의 정서에 따라 섭취하는 컴포트 푸드의 종류를 살펴본 결과, 부정적 정서를 경험하는 상황에서는 남녀 모두 주로 술, 초콜릿, 매운 음식 그리고 음료를 선호하고 있었으며, 긍정적 정서를 경험하는 상황에서는 남녀 모두 치킨이 공통적으로 선호되었으며 여학생들은 다양한 음식들을 컴포트 푸드로 선택한 것에 반해 남학생들은 술을 주요 상위권의 음식으로 선택하고 있었다. 마지막으로 본 연구의 제한점과 후속연구에 대해서 논의하였다.

This study was conducted to examine the optimal concentration and treatment time of antioxidants for inhibition of the ROS generation in bovine somatic cell nuclear transfer (SCNT) embryos. Bovine oocytes were activated parthenogenetically, during which oocytes were treated with various antioxidants to determine the optimal concentrations and kind of antioxidants. Determined antioxidants were applied to oocytes during in vitro maturation (IVM) and/or SCNT procedures. Finally, antioxidant-treated SCNT embryos were compared with in vitro fertilized (IVF) embryos. H2O2 levels were analyzed in embryos at 20 h of activation, fusion or insemination by staining of embryos in 10 μM 2'7'-dichlorodihydrofluorescein diacetate (H2DCFDA) dye, followed by fluorescence microscopy. H2O2 levels of parthenogenetic embryos were significantly lower in 25 μM β- mercaptoethanol (β-ME), 50 μM L-ascorbic acid (Vit. C), and 50 μM L-glutathione (GSH) treatment groups than each control group (24.0±1.5 vs 39.0±1.1, 29.7±1.0 vs 37.0±1.2, and 32.9±0.8 vs 36.3±0.8 pixels/embryo, p<0.05). There were no differences among above concentration of antioxidants in direct comparison (33.6±0.9～35.2±1.1 pixels/embryo). Thus, an antioxidant of 50 μM Vit. C was selected for SCNT. H2O2 levels of bovine SCNT embryos were significantly lower in embryos treated with Vit. C during only SCNT procedure (26.4±1.1 pixels/embryo, p<0.05) than the treatment group during IVM (29.9±1.1 pixels/embryo) and non-treated control (34.3±1.0 pixels/embryo). Moreover, H2O2 level of SCNT embryos treated with Vit. C during SCNT procedure was similar to that of IVF embryos. These results suggest that the antioxidant treatment during SCNT procedures can reduce the ROS generation level of SCNT bovine embryos.

Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on kainic acid (KA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to 5 μg/ml inhibited KA (500 μM)-induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF (0.5 mug/ml) inhibited glutamate release into medium induced by KA (500 μM), which was measured by HPLC. Pretreatment of MF (0.5 mug/ml) inhibited KA (500 μM)-induced elevation of cytosolic calcium concentration ([Ca2+]c), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents KA-induced neuronal cell damage in vitro.