‘Baekseung’, a new variety of Flammulina velutipes, was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in Mushroom Research Division, Baekseung ARES in 2016. Baekseung showed fast mycelial growth and high mycelial density on MEA (Malt Extract Agar) media for 7days of incubation. Spawn running period on the sawdust substrate required 30days at 25°C. The cultivation period and optimum temperature were 11±1 days at 14°C for primordia formation and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in Baekseung harvested in optimal stage exhibited 11.3±0.4㎜ and 89.2±7.1㎜ and Megumi harvested in optimal stage showed 8.2±1.0㎜ and 95.9±5.0㎜ respectively. Yield of Baekseung and Megumi strain grown of sawdust substrate was 153.7±12.5g and 150.5±29.7g per 850ml in bottle cultivation. The inferred tree exhibited the difference of phylogenetic relationship between the Korean white fruiting body strains such as Baekseung, Uri1ho, Fv-14-a-38, and Fv-14-a-51 and the Japanese white fruiting body strain Megumi.