Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the efficiency of transfection needs to be improved. In the present study, three transfection protocols - non-liposomal transfection (NLT), magnetofection and electroporation - were compared by analysis of their transfection efficiencies and cell viabilities using human dental pulp cells (hDPC) and bovine fetal fibroblasts (bFF) as cell sources. Enhanced green fluorescent protein gene was used as the delivery indicator. For magnetofection, Polymag reagent was administrated. NLT, FuGENE-HD and X-treme GENE 9 DNA transfection reagents were used for NLT. For electroporation, the NeonTM and NEPA21TM electroporators were tested. NeonTM electroporation showed highest transfection efficiency when compared with NLT, magnetofection, and NEPA21TM electroporation, with transfection efficiency of about 33% in hDPC and 50% in bFF, based on viable cell population in each cell type. These results suggest that transfection by NeonTM electroporation can be used to deliver foreign genes efficiently in human and bovine somatic cells.

This study was focused on improvement of milk production in Mongolian dairy industry by artificial insemination (AI) technology, supported by ODA of KOICA in Republic of Korea. This program was started in January 2009 and it is in years. This manuscript summarized the data especially on estrus synchronization and pregnancy establishment in dairy cows (Holstein) this year. A total of 81 dairy cows from 4 private farms (38 from Undarmal milk and that of 30, 8 and 5 dairy cows from Onjin (Enkhbayer), Jargalant, and BRM School farms respectively) were synchronized with 5 ml Lutalyse (i.m.) in the dump of dairy cows and then estrus was detected 2 to 3 days after injection. The synchronized dairy cows were inseminated with 0.5 ml dairy frozen semen by conventional artificial insemination (AI) techniques. Pregnancy was diagnosed about 60 days after AI by palpation method. About 96.3% (78/81) of synchronized cows were responded to single injection. Total 75 over 78 dairy cows (90.1%) inseminated were diagnosed as pregnant. The estrus induction and pregnancy rates were very effective using Lutalyse injection and conventional AI techniques in Mongolian dairy cow. The present results indicated that AI after estrus induction in Mongolian dairy cows could be applied to dairy breeding technology for improving breeding efficiency and milk production of the country.

웨이브렛 변환을 이용한 인공위성 영상의 압축을 보다 효과적으로 수행할 수 있는 필터의 조합을 살펴보았다. 압축정도를 표현하는 방법으로 encoding ratio를 이용하였고, 웨이브렛의 선택에 따라 PSNR값과 encoding ratio값이 변하는 양상을 보았다. 웨이브렛의 선택에 따라 PSNR값은 대략 13.2-21.6% 정도의 차이를 보였고 encoding ratio는 16.8-45.5%까지의 차이를 보였다. SAR 영상의 경우 encoding ratio는 16%20% 변동이 있지만, 일반영상의 경우는 웨이브렛의 선택에 따라 40% 이상 변화였다. 영상 압축시 웨이브렛의 선택효과는 인공위성 영상보다 일반영상에서 큰 영향을 미쳤다. PSNR, encoding ratio의 지수에서 인공위성 영상은 웨이브렛 선택에 영향을 덜 받는다. 인공위성 영상의 압축에 대한 웨이브렛 선택효과를 보여주기 위하여 새로운 지수인 ECR을 제안하였다. ECR은 일반영상보다 인공위성영상에서 웨이브렛의 종류에 따른 영향이 더 적게 나타났다. 그러나 인공위성 영상 압축시 3개의 지수에서 웨이브렛의 선택효과는 최소한 10% 이상으로 선택의 중요성은 무시될 수 없을 것이다.

The microstructures and mechanical properties of Dual Phase (DP), Transformation-Induced Plasticity (TRIP), and Quenching & Partitioning (Q&P) steels were investigated in order to define the strengthening mechanism of 0.2 C steel. An intercritical annealing between Ac1 and Ac3 was conducted to produce DP and TRIP steel, followed by quenching the DP and TRIP steel being quenched at to room temperature and by the TRIP steel being austemperingaustempered-air cooling cooled the steel toat room temperature, respectively. The Q&P steel was produced from full austenization, followed by quenching to the temperature between Ms and Mf, and then enriching the carbon to stabilize the austenite throughout the heat treatment. For the DP and TRIP steels, as the intercritical annealing temperature increased, the tensile strength increased and the elongation decreased. The strength variation was due to the amount of hard phases, i.e., martensite and bainite, respectively in the DP and TRIP steels. It was also found that the elongation also decreased with the amount of soft ferrite in the DP and TRIP steels and with the amount of the that was retained in the austenite phasein the TRIP steel, respectively for the DP and TRIP steels. For the Q&P steel, as the partitioning time increased, the elongation and the tensile strength increased slightly. This was due to the stabilized austenite that was enriched with carbon, even when the amount of retained austenite decreased as the partitioning time increased from 30 seconds to 100 seconds.

Bone marrow (BM) cell harvesting is a crucial element in the isolation of mesenchymal stem cells (MSCs). A simple method for harvesting cat BM cells is described. The results show that a large number of BM cells can rapidly be harvested from the cat by this simple procedure. MSCs prepared by density-gradient method were spindle-shaped morphology with bipolar or polygonal cell bodies and strongly positive for CD9 and CD44 and negative for CD18 and CD45-like. They were capable of differentiation to adipocytic and osteocytic phenotypes when exposed to appropriate induction media. The advantages of this method are its rapidity, simplicity, low invasiveness, and low donor attrition and good outcome.

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

TRiplet Ionospheric Observatory-CubeSat for Ion, Neutron, Electron & MAgnetic fields (TRIO-CINEMA) is a CubeSat with 3.14 kg in weight and 3-U (10 × 10 × 30 cm) in size, jointly developed by Kyung Hee University and UC Berkeley to measure magnetic fields of near Earth space and detect plasma particles. When a satellite is launched into orbit, it encounters ultrahighvacuum and extreme temperature. To verify the operation and survivability of the satellite in such an extreme space environment, experimental tests are conducted on the ground using thermal vacuum chamber. This paper describes the temperature control device and monitoring system suitable for CubeSat test environment using the thermal vacuum chamber of the School of Space Research, Kyung Hee University. To build the chamber, we use a general purpose thermal analysis program and NX 6.0 TMG program. We carry out thermal vacuum tests on the two flight models developed by Kyung Hee University based on the thermal model of the TRIO-CINEMA satellite. It is expected from this experiment that proper operation of the satellite in the space environment will be achieved.

The detector subsystem of the Optical Wide-field Patrol (OWL) network efficiently acquires the position and time information of moving objects such as artificial satellites through its chopper system, which consists of 4 blades in front of the CCD camera. Using this system, it is possible to get more position data with the same exposure time by changing the streaks of the moving objects into many pieces with the fast rotating blades during sidereal tracking. At the same time, the time data from the rotating chopper can be acquired by the time tagger connected to the photo diode. To analyze the orbits of the targets detected in the image data of such a system, a sequential procedure of determining the positions of separated streak lines was developed that involved calculating the World Coordinate System (WCS) solution to transform the positions into equatorial coordinate systems, and finally combining the time log records from the time tagger with the transformed position data. We introduce this procedure and the preliminary results of the application of this procedure to the test observation images.

Photometric observation is one of the most effective techniques for determining the physical characteristics of unknown space objects and space debris. In this research, we examine the change in brightness of the Communication, Ocean, Meteorological Satellite-1 (COMS-1) Geostationary Orbit Satellite (GEO), and compare it to our estimate model. First, we calculate the maximum brightness time using our calculation method and then derive the light curve shape using our rendering model. The maximum brightness is then calculated using the induced equation from Pogson's formula. For a comparison with our estimation, we carried out photometric observation using an optical telescope. The variation in brightness and the shape of the light curve are similar to the calculations achieved using our model, but the maximum brightness shows a slightly different value from our calculation result depending on the input parameters. This paper examines the photometric phenomenon of the variation in brightness of a GEO satellite, and the implementation of our approach to understanding the characteristics of space objects.

Triplet Ionospheric Observatory (TRIO) CubeSatforIon, Neutral, Electron MAgneticfields (CINEMA) is a CubeSat with the weight 3 kg that will be operated in the orbit conditions of about 800 km altitude and 90° inclination angle, using the S-band and ultra-high frequency (UHF)-band communication frequencies. Regarding the communication antenna loaded on the satellite, the two patch antennas has the downlink function in the S-band, whereas the two whip antennas has the function to receive the command sent by the ground station to the satellite in the UHF-band. The uplink ground station that communicates through the UHF-band with the CINEMA satellite was established at Kyung Hee University. The system is mainly composed of a terminal node controller, a transceiver, and a helical antenna. The gain of the helical antenna established at the Kyung Hee University ground station was 9.8 dBi. The output of the transceiver was set to be 5 W (6.9 dB) for the communication test. Through the far-field test of the established system, it was verified that the Roman characters, figures and symbols were converted into packets and transmitted to the satellite receiver in the communica\-tion speed of 9,600 bps.

Thermal analysis and control design are prerequisite essential to design the satellite. In the space environment, it makes satellite survive from extreme hot and cold conditions. In recent years CubeSat mission is developed for many kinds of purpose. Triplet Ionospheric Observatory (TRIO)–CubeSat for Ion, Neutral, Electron, MAgnetic fields (CINEMA) is required to weigh less than 3 kg and operate on minimal 3 W power. In this paper we describe the thermal analysis and control design for TRIO-CINEMA mission. For this thermal analysis, we made a thermal model of the CubeSat with finite element method and NX6.0 TMG software is used to simulate this analysis model. Based on this result, passive thermal control method has been applied to thermal design of CINEMA. In order to get the better conduction between solar panel and chassis, we choose aluminum 6061-T6 for the material property of standoff. We can increase the average tempera\-ture of top and bottom solar panels from -70°C to -40°C and decrease the average temperature of the magnetometer from +93°C to -4°C using black paint on the surface of the chassis, inside of top & bottom solar panels, and magnetometer.

The present study examines the morning-afternoon asymmetry of the geosynchronous magnetic field strength on the dayside (magnetic local time [MLT] = 06:00~18:00) using observations by the Geostationary Operational Environmental Satellites (GOES) over a period of 9 years from February 1998 to January 2007. During geomagnetically quiet time (Kp < 3), we observed that a peak of the magnetic field strength is skewed toward the earlier local times (11:07~11:37 MLT) with respect to local noon and that the geosynchronous field strength is larger in the morning sector than in the afternoon sector. That is, there is the morning-afternoon asymmetry of the geosynchronous magnetic field strength. Using solar wind data, it is confirmed that the morning-afternoon asymmetry is not associated with the aberration effect due to the orbital motion of the Earth about the Sun. We found that the peak location of the magnetic field strength is shifted toward the earlier local times as the ratio of the magnetic field strength at MLT = 18 (B-dusk) to the magnetic field strength at MLT = 06 (B-dawn) is decreasing. It is also found that the dawn-dusk magnetic field median ratio, B-dusk/B-dawn, is decreasing as the solar wind dynamic pressure is increasing. The morning-afternoon asymmetry of the magnetic field strength appears in Tsyganenko geomagnetic field model (TS-04 model) when the partial ring current is included in TS- 04 model. Unlike our observations, however, TS-04 model shows that the peak location of the magnetic field strength is shifted toward local noon as the solar wind dynamic pressure grows in magnitude. This may be due to that the symmetric magnetic field associated with the magnetopause current, strongly affected by the solar wind dynamic pressure, increases. However, the partial ring current is not affected as much as the magnetopause current by the solar wind dynamic pressure in TS-04 model. Thus, our observations suggest that the contribution of the partial ring current at geosynchronous orbit is much larger than that expected from TS-04 model as the solar wind dynamic pressure increases.