Miniature pigs have been marked as a source of providing organs for xenotrans plantation. In order to produce gnotobiotic miniature pigs in large numbers and perform experiments, it is necessary to implement a specially designed isolator that provides a space completely isolated from the outside, enabling researchers to control the microbiological environment. Conventional isolators have gaps on the floor net that cause accidents such as pigs getting their hooves caught. Some pigs have shown maldevelopment and abnormalities in the hind limbs. The goal of this study was to design an isolator floor for secure the sole of a foot while swine had not received undue stress with slippery flooring. We created a gradient on the isolator floor and used a single-body panel to eliminate gaps and used a checkered panel to prevent pigs from slipping. We also implemented an exhaust at the end of the gradient to collect excrement and urine. Raising miniature pigs for five months in the isolator floor invented by this study. This study compared the serum chemistries, cortisol levels to physiologic evaluations and lesions on their soles. Serum cortisol value was higher in conventional isolator floor pigs than newly developed isolator floor pigs. We observed lesions on their soles of a foot but we found no lesions and abnormalities in new type of isolator floor. This new type of isolator floor can secure the sole of a foot in pigs. The current design is developed for miniature pigs in isolator floor, but it can easily be adapted for other animals or other fields.