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        검색결과 2

        2.
        2010.04 KCI 등재 서비스 종료(열람 제한)
        The SSR markers were generally used to analysis the plant genetic diversity, but it have been rarely reported in case of castor bean. We constructed the microsatellite-enriched genomic library and sequenced totally 775 clones to obtain the microsatellite sequence information of the castor bean. Among the sequenced clones, one hundred fifty clones (19.3%) were redundant and four hundred twenty (67.2%) were found to contain microsatellite sequences within the remaining 625 unique clones. A total of 237 primer pairs were designed based on the sequenced microsatellite clones information and evaluated for polymorphism in ten castor bean accessions. Twenty-eight SSR markers produced reproducible polymorphic bands and were further characterized using a diverse set of 25 castor bean accessions. The majority of unique SSRs revealed dinucleotide motives (84%) on the other hand the ratio of trinucleotide motives was 15%. A microsatellite enriched library from the Ricinus communis L. was mainly consisted of [(AG), (GA)/(CT), (TC)] and [(CTT)/(AAG)] microsatellite motifs. The length of dinucleotide SSRs ranged from 4 to 50 repeats with an average 12.4, and that of trinucleotide SSRs from 4 to 56 with an average of 7.35 repeats. These newly developed microsatellite markers will be useful for breeding system and classification of Ricinus communis L.