This study was to investigate pregnancy rate of IVM/IVF/IVC Korean cattle (registered in government) embryos according to transport time course. For the production of embryos, oocytes recovered from slaughtered excellent grade cow and highly motile frozen‐thawed bull semen (purchased from LIMC, KPN#497) was used. In vitro produced embryos were cultured in CR1aa medium for 8 days and some of them were frozen. The rate of average cleavage (>2‐cell) was 83.0% (308/371) and blastocyst rate at day 8 was 34.7% (107/308). Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. These embryos were produced in a laboratory, embryo transfer (ET) was planned in 10 areas of the remote island (Jeju) from the laboratory by airplane. Thus, we examined the pregnancy rate in recipient cow according to embryo of transport time course before ET. From embryo transferred 44 recipient cows, overall pregnancy was 40.9% (18/44), these 18 cows were all calved [single, 94% (17/18); twin, 6% (1/18)] and total embryo implantation rate was 26% (19/66). Comparing transport time in the base of 6 hr, pregnancy rate in ET group required less 4 hr (60%, 9/15) was significantly higher than that required more 6 hr (26.3%, 5/19). In direct ET of freezing embryos, the pregnancy rate was 40% (4/10). However, it was difficult to find the meaning of temperature, pH and corpus luteum quality of recipients on comparison of pregnancy rate. When the cell death level of embryos according to storage time in thermos (straw container) before ET was measured by TUNEL staining, apoptotic index was increased with storage time‐dependent. These results demonstrated that long distance transfer of IVM/IVF/IVC embryos is possible and the time of embryo transport is very important for the pregnancy rate on field trial.

Advertisement signboards on the buildings have functionally a visual value as factor of urban and building design. But it is not easy to find any reasonable principles and regulations, how well the signboards deliver the information of stores to the customers, and how they contribute the visual amenity from the sight of urban landscape. Under this context, this study tries to analyze the information delivery power of signboards in relation to the visual cognition. The analysis method is the Two-Way ANOVA (Analysis of Variance) evaluation with factors, such as scale, amount and arrangement of signboards. The scale of letters or figures at Signboards is specified in 3 different sizes such as big, medium and small. The amount is classified into 5 grades from 100% to 20% installation. And the arrangement is divided into orderly and disorderly installation. As the results of the Two-Way ANOVA, the identical scale of signboards is much better for the awareness. And the orderly arrangement shows also the same result. Above all, including these two results, the 80% of installation showed the biggest different result of awareness.