Several plant growth regulators were examined for on their effect on the in vitro propagation of Kalopanax pictus(THUNB.) (NAKAI.) on WPM medium. Among the cytokinins tested, BA at 13.32 μM appeared to be the most effective for multiple axillary shoot formation. Although the addition of 2.89 μM GA3 promoted stem elongation, it produced morphologically abnormal leaves and stems. For rooting of the shoots, 4.9 μM IBA seemed to be more effective than 2.69 μM NAA. When the regenerated plants were transferred on artificial mixture containing vermiculite and peat-moss (1 : 1, v/v), 81% of them survived and grew normally.