A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format O(1-6)XXXXX-NH2, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-NH2 (pep-1) and WXXXXN-NH2 (pep-2), and one positioned peptide, RRRWRN-NH2 (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic α-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.

본 논문에서는 FR-ECC의 수축특성과 균열도입 전후의 내동해성을 평가하였으며, FR-ECC를 활용한 다층복공구조의 지수성능과 박리박락저항성, 또한 FR-ECC로 단면복구 된 보부재의 휨성능을 평가하였다. 그 결과, FR-ECC는 소성수축에 의한 균열 및 건조수축에 의한 길이변화율이 기존의 보수모르터에 비해 크게 저감되었으며, 구속상태에서의 건조수축에 대한 균열저항성이 개선됨을 알 수 있었다. 또한, FR-ECC는 내동해성이 매우 우수하였으며, 균열도입 후에도 동결융해작용에 의한 인장성능의 저하는 확인되지 않았다. 한편, FR-ECC로 보수된 휨부재는 초기균열모멘트, 항복모멘트 및 극한모멘트 등의 휨성능이 증대되었으며, 멀티플크랙 특성에 의해 휨파괴시까지 균열폭을 안정적으로 제어할 수 있었다.