Recently, Free-Form and Irregular Shape high-rise buildings are constructed by IT technology development. Tilted shaped high-rise building which is one of Irregular shape high-rise buildings can cause lateral displacement by gravity load and lateral load due to tilted elevation shape. Therefore, it is necessary to review the behavior and structural aspects of the Tilted shape high-rise building by gravity load. In this paper, the dynamic characteristics of a tilted structure with a dual-core were analyzed with the core location as a design variable, and response behavior, vulnerable members, and vulnerable layers to earthquake loads were analyzed. As a result of the analysis, as the location of the core moved in an tilted direction, the eccentric distance and eccentric load decreased, reducing the axial force of the vertical members. However, the location of the core had little effect on the response.

Hertwig's epithelial root sheath (HERS) consists of bilayered cells derived from the inner and outer dental epithelia and plays important roles in tooth root formation as well as in the maintenance and regeneration of periodontal tissues. With regards to the fate of HERS, and although previous reports have suggested that this entails the formation of epithelial rests of Malassez, apoptosis or an epithelialmesenchymal transformation (EMT), it is unclear what changes occur in the epithelial cells in this structure. This study examined whether HERS cells undergo EMT using a keratin-14 (K14) cre:ROSA 26 transgenic reporter mouse. The K14 transgene is expressed by many epithelial tissues, including the oral epithelium and the enamel organ. A distinct K14 expression pattern was found in the continuous HERS bi-layer and the epithelial diaphragm were visualized by detecting the β-galactosidase (lacZ) activity in 1 week postnatal mice. The 2 and 4 week old mice showed a fragmented HERS with cell aggregation along the root surface. However, some of the lacZ-positive dissociated cells along the root surface were not positive for pan-cytokeratin. These results suggest that the K14 transgene is a valuable marker of HERS. In addition, the current data suggest that some of the HERS cells may lose their epithelial properties after fragmentation and subsequently undergo EMT.

The working mechanism of bisphosphonate on bone cells is unclear despite its powerful inhibitory activity on bone resorption. The differentiation and activation of osteoclasts are essential for bone resorption and are controlled by the stimulatory RANKL and inhibitory OPG molecules. Teeth exhibit a range of movement patterns during their eruption to establish their form and function, which inevitably accompanies peripheral bone resorption. Hence, the mandible, which contains the teeth during their eruption processes, is a good model for revealing the inhibitory mechanism of bisphosphonate upon bone resorption. In the present study, RANKL and OPG expression were examined immunohistochemically in the mandible of rats with developing teeth after alendronate administration (2.5 mg/kg). The preeruptive mandibular first molars at postnatal days 3 to 10 showed the developing stages from bell to crown. No morphological changes in tooth formation were observed after alendronate administration. The number of osteoclasts in the alveolar bone around the developing teeth decreased markedly at postnatal days 3, 7 and 10 compared with the control group. RANKL induced strong positive immunohistochemical reactions in the dental follicles and stromal cells around the mandibular first molar. In particular, many osteoclasts with strongly positive reactions to RANKL appeared above the developing mandibular first molars at postnatal days 3 and 10. Immunohistochemical reactions with RANKL after alendronate administration were weaker than the control groups. However, the immunohistochemical reactivity to OPG was stronger after alendronate administration, at postnatal days 3 and 10. These results suggest that alendronate may decrease bone resorption by regulating the RANKL/OPG pathway in the process of osteoclast formation, resulting in a delay in tooth eruption.

Teeth develop via a reciprocal induction between the ectomesenchyme originating from the neural crest and the ectodermal epithelium. During complete formation of the tooth morphology and structure, many cells proliferate, differentiate, and can be replaced with other structures. Apoptosis is a type of genetically-controlled cell death and a biological process arising at the cellular level during development. To determine if apoptosis is an effective mechanism for eliminating cells during tooth development, this process was examined in the rat mandible including the developing molar teeth using the transferase-mediated dUTP-biotin nick labeling (TUNEL) method. The tooth germ of the mandibular first molar in the postnatal rat showed a variety of morphological appearances from the bell stage to the crown stage. Strong TUNEL-positive reactivity was observed in the ameloblasts and cells of the stellate reticulum. Odontoblasts near the prospective cusp area also showed a TUNEL positive reaction and several cells in the dental papilla, which are the forming pulp, were also stained intensively in this assay. Our results thus show that apoptosis may take place not only in epithelial-derived dental organs but also in the mesenchyme-derived dental papilla. Hence, apoptosis may be an essential biological process in tooth development.

최근 집중호우의 발생빈도가 증가하고 있으며, 이를 고려한 강우분석을 실시하여야 한다. 현재 수문설계를 위한 강우분석은 한반도 조밀도 36 km 인 기상청 관할 종관기상관측지점(Automated Surface Observing System, ASOS)의 시 단위 강우를 이용하고 있다. 이로 인해 같은 강우지점의 티센망에 포함되는 중소규모 유역은 동일한 확률강우량과 강우시간분포로 분석하게 됨으로 유역특성을 고려하지 못하는 문제가 발생한다. 또한, 10~20 km 범위 내에서 발생하는 집중호우의 시 ․ 공간적 변화를 고려하지 못하는 문제점이 발생한다. 따라서 본 연구에서는 종관기상관측지점에 비해 상대적으로 조밀도가 우수한 방재기상관측지점(Automatic Weather System, AWS)의 분 단위 강우자료를 이용하여 집중호우를 고려한 확률강우량을 산정하였다. 또한, 유역에 적합한 Huff의 4분위 방법 산정을 위해 Case별 시간분포 산정과 유출분석을 실시하였다. 이는 집중호우와 유역특성을 반영한 설계수문량 산정에 크게 기여할 것으로 판단된다.

대부분의 수문분석은 시 단위 강우를 기반으로 확률강우량과 강우시간분포를 산정하고, 확률강우량과 강우시간분포의 자료기간을 달리 적용하는 방법으로 강우-유출분석을 수행하고 있다. 본 연구에서는 자료형태(시 단위와 분 단위 강우자료)와 확률강우량과 강우시간분포의 다른 자료기간 적용에 따른 설계홍수량 변화를 정량화 하고자, 자료형태와 자료기간에 따라 지점빈도해석을 통한 확률강우량 산정과 Huff의 4분위 방법을 통한 강우시간분포를 산정하였다. 또한, 확률강우량과 설계강우 시간분포의 자료기간을 달리 적용한 강우-유출분석으로 설계홍수량 변동분석을 실시하였다. 분석결과, 자료형태에서는 분 단위 강우가 시 단위 강우보다 더욱 정확하고 효과적인 강우분석을 수행할 수 있는 것으로 나타났으며, 확률 강우량과 강우시간분포의 다른 자료기간을 적용하여 산정된 설계홍수량의 차 보다 자료형식에 따른 설계홍수량 결과가 보다 큰 차이를 보이는 것 으로 나타났다. 이는 향후 분 단위 강우를 활용한 수문분석에 크게 기여할 것으로 판단된다.