본 연구는 엘크 암사슴(Cervus canadensis)의 사육방식에 따른 사료섭취량, 체중변화 및 자록의 성장에 미치는 영향을 확인하고 적정 방목강도를 구명하기 위하여 수행되었다. 본 실험에 사용 된 공시가축은 3~4년생 엘크 암사슴 16두(평균체중 : 236.2 ± 15.7 kg)를 이용하였으며, 방목초지는 3년 이상된 톨 페스큐 위주의 기성 혼파초지로서 초종구성은 톨 페스큐(약 50%), 오차드 그라스(약 10%), 켄터기 블루그라스(약 5%)와 피, 바랭이 등으로 구성되었다. 방목초지의 수분함량은 19.51∼22.61%로 방목기간 동안 유사하였으며, 조단백질 함량은 6~7월에 유의적으로 높게 나타났다. 조지방과 조회분 함량은 가을로 갈수록 점차 증가하였으며, NDF와 ADF함량은 각각 53.65∼60.18%과 26.08∼29.10% 로 조사되었다. 실험기간동안 보충사료 섭취량은 월별로 처리구 사이에 유의적인 차이가 나타나지 않았으나, 조사료와 총 건물섭 취량은 5월을 제외하고 처리구 사이에 유의적인 차이가 나타났다 (p<0.05). 특히 8월에는 GR처리구의 조사료 섭취량이 급격히 감소하였는데, 여름철 환경변화에 따른 것으로 사료된다. 반면, 9월 포유를 위해 부족한 영양소를 섭취하기 위해 사초섭취량이 급격히 증가하였다. 우리 연구에서 암사슴의 채식패턴은 수사슴과 차이를 보였으며, 이는 실험기간 자록의 이유 및 포유기간에 따른 영향을 받은 것으로 사료된다. 본 실험에서 사양방식에 따른 엘크 암사슴의 체중은 유의적 차이가 나타나지 않았으나, GR처리구에서 자록의 성장률이 빠른 것으로 나타났다. 그러나, GR처리구에서 BF처리구에 비해 낮은 이유율이 나타나 분만 전후 사사사육을 통해 이유율을 높여주는 것이 필요할 것으로 판단되며, 목지 내에서 분만 시 포유경험이 있는 산차가 높은 암사슴을 이용하고 방목강도를 높여주는 것이 이유율을 향상시키는 데 도움을 줄 수 있을 것으로 사료된다. 엘 크 암사슴의 방목강도는 연평균 15두/ha로 나타났는데, 이는 다마 암사슴(Dama dama)에 비해서는 체중대비 높게 나타났으나, 엘크 수사슴에 비해서는 낮은 수치를 보여주었다. 이러한 결과는 기성초지 이용으로 인해 사초의 생산성이 감소한 결과로 사료되며, 앞으로 방목초지의 혼파방법과 기성초지의 비배관리 등을 통해 초지 활용성을 높일 수 있는 연구가 추가적으로 필요할 것으로 사료된다.

In this study, the effect of forage sources in the total mixed ration (TMR) on in vitro goat rumen fermentation was investigated. Rice straw (RS), Italian ryegrass (IRG), timothy (TIM), and alfalfa (ALF) were used as forage sources. Each forage source was mixed with a commercial goat concentrate diet in the ratio of 1:1. Total 4 TMR were prepared. Rumen simulated in vitro fermentation using goat rumen fluid collected from the slaughterhouse was conducted until 72 th . For fermentation parameters, gas production (GP), volatile fatty acids (VFAs), and ammonia nitrogen (NH3-N) were examined. All assays were performed at 24 th , 48 th , and 72 th h of incubation individually. Contents of crude protein and non-fibrous carbohydrate were greater in the order of RS < IRG < TIM < ALF. Significant treatment effects were found in valerate and NH3-N at 24 th h of incubation (p<0.05). ALF showed the greatest contents of them and RS was the lowest. At 48 th incubation, a significant effect was detected at GP (p<0.05) and RS was greater than others. However, GP of RS was lower than others at 72 th . Significant effects on Total VFA, butyrate, and valerate productions were found at 72 th h of incubation (p<0.05). ALF showed the greatest production. Methane production from all treatments was not significantly different for each incubation time (p>0.05). The present study provided primary information on how goat rumen fermentation responds to different nutrient contents and forage sources of TMR. And the information could be used for the design or optimizing economical diet formulation for goats.

Data on the crude protein requirements of elk doe are nonexistent and the data are essential for their management in Korea. Therefore, this study was conducted to evaluate the crude protein requirement for maintenance of elk doe. Three female elk deer were used in 3 × 3 Latin square design with three diets containing three levels of crude protein (CP) that contained low crude protein (approximately 12%), medium crude protein (15%), and high crude protein (18%). Each three elk doe trials included a 14-day preliminary period and a 5-day collection period. Crude protein intake was 4.83, 6.26, and 9.00 g/d for 12%, 15%, and 18% of CP level, respectively. Crude protein balances were 1.04, 1.41, and 4.14 for 12%, 15%, and 18% of CP level, respectively. The maintenance requirement for CP from the regression equation between CP intake and CP balance were 3.70 g/BW0.75.

본 연구에 이용되는 국내 재래 흑염소는 유전적 자원을 보존하고 품종을 유지하기 위하여 소규모 집단에서 고도의 근친교배를 수행하고 있어 유전적 자질의 다양성이 저해되고 있을 것으로 우려되었다. 따라서 본 연구는 국내 재래흑염소 경상대계통 집단의 유전체 정보를 활용하여 재래흑염소 집단의 유전적 특성과 유효집단의 크기를 추정하고자 실시하였다. 국내 재래 흑염소의 유전체 정보는 Illumina Goat SNP 50k chip (illumina, inc., San Diego, CA)의 정보를 분석하여 연구에 이용하였다. 각 염색체의 인접 표지인자 와의 연관불평형 (Linkage Disequilibrium)은 0.225로 추정이 되었다. 또한, 표지인자 사이의 거리가 증가함에 따라 연관불평형의 값은 감소되는 것으로 나타났다. 국내 재래 흑염소의 유효집단크기는 최근의 세대로 오는 경우 감소하는 추세를 보였으며, 13세대에서 유효집단의 크기는 29두로 추정되었다. 재래 흑염소 경상대계통은 낮은 연관불평형 값과 유효집단 크기를 보여 유전적 자원의 다양성이 낮을 것으로 보이며, 이를 해결하기 위한 계획적인 교배와 품종 집단의 크기를 키우기 위한 대책이 필요할 것으로 사료된다.

The normal feeding approach of goats might be due to their precise anatomical and physiological characteristics of entity, which permit them to be highly selective, to eat legume silages and wild green grass. This review has been designed to consider the grazing behavior, fodder selection, and feed composition of goats. Various herbs and corns consumed by goats have numerous nutritive resources. Based on the general herbaceous intake activities and behavior of goats, they prefer wild grass such as grass grown in the steep hills than soft grass. Because the digestion capacity of cellulose feed has higher digestion level compared to other non-ruminants within rumen and it is advantageous to use wild forest or mountain grass which comprises high proportion of cellulose feed for goat. In South Korea, there are abundant feed resources for goats because of occupying large areas of mountains. Thus, goat production and feeding costs could be reduced if plants are used from the wild forest as a feed for goats relative to grassland grazing. Also, it is expected to contribute in improvement of goat farming with harmonious relationship between the grassland and wild forest while satisfying animal welfare and physiological desires of livestock.

엘크 수사슴의 체중 성장곡선 및 일당증체량을 추정하기 위하여 국립축산과학원 가축유전자원센터에서 사육중인 엘크 수사슴 183두의 체중-일령 자료 1,509개에 대해 비선형 회귀식인 Gompertz와 Logistic 모형을 적용하였다. 추정된 엘크 수사슴 성장곡선 모수인 성숙체중(A), 성장비율(b)과 성숙률(ｋ)은 Gompertz 모형의 경우 각각 335.5±1.415kg, 2.18±0.045와 0.0025±0.00005였고, Logistic 모형에서는 330.5±1.337kg, 5.46±0.209와 0.0355±0.00009였다. 적합도 검정을 위한 결정계수(R2)는 두 모형에서 큰 차이가 없어 두 모형 다 적합한 것으로 나타났다. 다만, 실제 측정값과 추정값에 대한 차이인 평균제곱근 오차 값이 엘크 암사슴에 비해 크게 나타났으며 따라서 엘크 수사슴의 낙각 및 번식기 체중 증감을 고려한 성장곡선 모형에 대한 추가적인 연구가 필요할 것으로 판단된다. 엘크 수사슴에서 Gompertz 모형으로 추정된 함수식은 f(t)=335.5Xe^-2.18e^-0.0025t였고, Logistic 모형의 경우 F(t)=330.5X(1+5.465e^-0.00355t)^-1였다. 각 모형에서 제1차 도함수로 유래된 일당증체량 곡선은 Gompertz 모형의 경우 F'(t)=0.0025Xf(t)X1n(335.5/f(t)), Logistic 모형의 경우f'(t)=0.0036Xf(t)X[1-f(t)/330.5]였다. 이 함수식을 통해 도출된 엘크 수사슴에서 최대 증체량을 나타내는 시기인 315~478.3일령, 123.4~165.3㎏ 도달 전후에는 보다 세심한 사양관리가 필요할 것으로 보인다. 본 연구에서 도출된 엘크 수사슴의 성장곡선 모수 추정값, 변곡점 및 변곡점 도달일령 등은 사슴산업 종사자들이 사양관리 및 육종계획을 수립하는 데에 도움이 될 수 있을 것으로 사료된다.

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold (4°C), salt (NaCl 200 mM) and heat (42°C) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

The objective of this study was to estimate the growth curve parameters of body weight for female Elk. Weight and age data from 115 does raised at the Animal Genetic Resources Research Center in Korea were used in this study. The growth curve parameters were estimated from a nonlinear regression using Gompertz and Logistic models. Mature weight (A), growth ratio (b) and maturing rate (k) of female Elk were 214.1±2.17 kg, 2.12±0.0045 and 0.0043±0.00011, respectively, according to the Gompertz model. They were 208.3±2.17 kg, 5.56±0.234 and 0.0065±0.00017, respectively, according to the Logistic model. The goodness of fit determined by R2 was higher in the Gompertz model than that in the Logistic model. The growth curve functions obtained from the Gompertz and Logistic models in female Elk were f(t)=214.1×e-2.124×e-0.00484×t and f(t)=208.3×(1+5.561×e-0.00651×t)-1, respectively. The absolute growth rate functions from the Gompertz and Logistic models in female Elk were f'(t)=0.0043×f(t)×1n(214.1/f(t)) and f'(t)=0.0065×f(t)×[1-f(t)/208.3]respectively. The growth pattern of female Elk generated from this study can be useful in determining the most appropriate feeding plans and the best breeding strategies for deer.

At present, oak sawdust and rice bran are mainly used as the medium materials for bag cultivation of Lentinula edodes. This experiment was conducted to develop the optimum medium of ‘Nongjingo’, a new variety of Lentinula edodes. We investigated oak sawdust, oak fermented sawdust, poplar sawdust, and douglas fir sawdust as main materials, and rice bran and wheat bran as nutrient resources, respectively. The medium composition was consisted of main material 80%+nutrient 20% based on the volume ratio. Eight treatments of medium composition were tested. The period of mycelial incubation in ‘oak sawdust 40%+douglas fir sawdust 40%+rice bran 20% was 86 days (39 days in dark incubation and 47 days in browning) shortened up to 18 days compared to the control. The 1st yield of commercial fruiting bodies in this treatment was 48.1 g/bag which was 44.8% higher than that in control. We expect that the cultivation period of ‘Nongjingo’ will be shortened, and the yield increased in the medium replaced half of oak sawdust by douglas fir sawdust.

This study was conducted to estimate growth curve parameters by sex in Korean native Goat. Weight-age data from 148 male goats and 169 female goats raised at Animal Genetic Resources Research Center in Korea were used in this study. Growth curve parameters were estimated from nonlinear regression using Gompertz and Logistic models. Mature weight ( ) and growth rate () of male goats were 50.0 kg, and 0.0038, respectively, when Gompertz model was used. They were 47.9 kg and 0.0062, respectively, when Logistic model was used. In female goats, mature weight ( ) and growth rate () were 35.8 kg and 0.0030, respectively, when Gompertz model was used. They were 34.6 kg and 0.0046 when Logistic model was used. The goodness of fit determined by R2 was higher in the Gompertz model than that in the Logistic model. The growth curve functions estimated from the Gompertz model in Korean-native male and female goats were , respectively. The inflection point () showed that the maximum growth rate and the weight at inflection (  ) estimated from the Gompertz model were 234.8 days and 18.37 kg, respectively, in male goats. They were 235.7 days and 13.16 kg, respectively, in female goats. The growth pattern of Korean-native goats obtained from this study might be useful for determining their feeding and management plans by sex and design breeding strategies.

In recent decades, there has been an increasing interest in the use of carbon fiber reinforced plastic (CFRP) in aerospace, renewable energy and other industries, due to its low weight and relatively good mechanical properties compared with traditional metals. However, due to the high cost of petroleum-based precursors and their associated processing costs, CF remains a specialty product and as such has been limited to use in high-end aerospace, sporting goods, automotive, and specialist industrial applications. The high cost of CF is a problem in various applications and the use of CFRP has been impeded by the high cost of CF in various applications. This paper presents an overview of research related to the fabrication of low cost CF using polyethylene (PE) control technology, and identifies areas requiring additional research and development. It critically reviews the results of cross-linked PE control technology studies, and the development of promising control technologies, including acid, peroxide, radiation and silane cross-linking methods.

In this study, carbonized fibers were prepared by using acidically cross-linked LDPE fibers. The surface morphologies of the carbonized fibers were observed by SEM. The effects of cross-linking process temperatures were studied using thermal analyses such as DSC and TGA. The melting and heating enthalpy of the fibers decreased as the cross-linking temperature increased. The cross-linked fibers had a carbonization yield of over 50%. From SEM results the highest yield of carbonized LDPE-based fibers was obtained by cross-linking at a sulfate temperature (170oC). As a result, carbonation yield of the carbonized fibers was found to depend on the functions of the cross-linking ratio of the LDPE precursors.