Various saponins are present in soybean seed, and they can be divided into three groups, group A, B and E saponin, in which the aglycone are soyasapogenol A, B and E, respectively. Group A saponins are supposed to be responsible for the undesirable bitter and astringent taste, and Group B saponins including 2,3-dihydro-2,5-dihydroxy-6-methyl- 4H-pyran-4-one (DDMP)-conjugated soyasaponins have the health-contributing activities. This study was conducted to investigate the saponin composition and content of 69 Korean cultivars. Thin-layer chromatography (TLC) and High-performance liquid chromatography (HPLC) were used to analyze the composition and content of soyasaponins. The composition of saponins in the seed hypocotyl of 69 Korean cultivars were divided into three groups, Aa, Ab and AaAb types. The numbers of Aa, Ab and AaAb types were 10, 36 and 23, respectively. The contents of group A saponin (Aa, Ab and Ac), group B saponin (non-DDMP, Ba and Bb) and DDMP saponin (αg and βg) ranged 21.7 ~ 57.0mg/g, 1.4 ~ 5.1mg/g and 9.8 ~ 27.4mg/g, respectively. A total of saponin content of seed hypocotyl in 69 Korean cultivars ranged from 43.8mg/g to 85.3mg/g with an average of 59.1mg/g.