The purpose of this study was to investigate the environmental factors that affect the computer literacy of childcare teachers. A survey was conducted to get information about teachers' ICT utilization ability and their physical and educational condition. Binary logistic regression analysis was performed using SPSS 17.0 program on the data of 293 teachers who work in childcare centers in capital area, and the following results were obtained. First, computer-related physical environment and computer training did not affect the teachers' computer literacy. Second, taking computer course had effects on the basic skills, word-processing ability, internet communication ability and computer program utilization. Third, the number of hours of using computers affected the ability to install and maintain computers. Fourth, the purchase of computer-related books and magazines and the number of computer programs they use had an effect on their computer program utilization. These results have significance in that they imply what is needed for improving childcare teachers' computer literacy in terms of institutional support.

Epithelial-mesenchymal transition(EMT) plays a pivotal role in the convers ion of earl y s tage tumors into invasive malignancies‘ and has been shown to be regulated hy the transcri ptional factor. Snail. Recent ly‘ actlvatlon of the phosphatidylinositol 3' kinase (PI3K)/따<:T axis is emerging as a centra l feature of EMT‘ However. it is unclear whether the phosphorylation of AKT regulate the expl'ession of s nail in ora l cancer cell underwent EMT. T。 investigate a role of p-AKT in EMT, we assessed the effects of inhibi ting p-AK1' activity in oral squamous can cer cells(KOSCC-25B) using PIAs, structurally modified phosphatidyli nositol ether lipid analogues(P1As) . PIAs de creased phosphorylation of c-Jun N-terrninal Kinase(JNK) and increased phosphorylation of glycogen synthase kinase 3beta(GSK-3beta). Inhibition of p-AKT ir빼ce d down regulation of Snail and Twist. but Sip1 regulated independent of p-AKT inhibition. Also inhibi tion of p-AKT dec reased cell migration and invas ion. Therefore our results implicate that p-AKT may contribute to the translocalization of sna il in the EMT associated with canceJ cell rnigration and invasion

Although a number of molecules have been implicated in the metastasis of oral squamous cell carcinoma (OSCC) , the precise molecular mechanisms that deterrnine the direction of rnigration and invasiveness of OSCC cells into the lymph nodes remain unclear, Chemokines are a superfarnily of small structurally related heparin- binding proteins‘ which have been identified as attractants that control the rnigration of leukocytes‘ especia lly during imrnune and inflammatory reactlOns Moreover, recent studies have demonstrated that several types 。f cancer express chemokine receptor‘s and use chemokines to metastasize to the target organ, However, there h ave been few reports on biological behaviors by downregulation 0 1' CXCR-4 in ora l cancel‘ cells We tried to screen several OSCC cell lines in order to obtain a suitable cell line model which had the cha ract eris tic of the constitutive ly expressed state of CXCR• 4 Of the several OSCC cell lines, only KOSCC-25B showed the high expression of CXCR-4 in both RT-PCR and Western blot analysis, siRNA-CXCR-4 infected subclones of KOSCC-25B(Si, 3‘ Si 1이 showed downregulation of CXCR-4 expression as expected‘ At serum-free co ndi tion‘ Si.3 s ubclone s ignif icantly decreased cell proliferations at 24 h and 48h and Si, lO subclone significant ly dec reased cell proliferations at 24 h Si ,3 clone dec reased to 67 ,4% and Si,lO clone to 65 ,5% in comparison to vector infected cells These data suggest that the downregulation of CXCR-4 expression could induce anti-rnigratory and ant i- rni g ratory effect