Research on alfalfa root morphology and health is very important in development of technology for efficient improvement and production of alfalfa. The objectives of this study were to evaluate the root morphology and health of two alfalfa varieties, Algonquin and Golden Queen, to determine relationships among root morphology traits and root health. Results from these analyses indicated t㏊t yield was 6.77-43.93 t/h㎡, total root length ranged from 215.17-708.89 ㎜, root surface area ranged from 93.61-717.06 ㎠, volume ranged from 3.24-57.72㎤. Results from these analyses indicated t㏊t root infestation score was the lowest averaged over age of alfalfa stand in Algonquin. Yield in 2-yr old stands was greater in Golden Queen compared to Algonquin.