Negative effects of insecticides on non-target organism can be reduced by using selective and less toxic insecticides. We assessed the effect of subsequent application of flonicamid and Beauveria bassiana GHA on pests and natural enemies in soybean fields by recording their density in 6-8 d interval. Flonicamid controlled Aphis glycines Matsumura in 6 day after spray (DAS). Nymphs of Riptortus pedestris (F.) were also reduced by 46 and 48% in 12 and 19 DAS, respectively. Application of B. bassiana reduced R. pedestris nymph 50% in 16 DAS whereas adult R. pedestris, Medythia nigrobilineata Motschulsky and A. glycines were not significantly reduced in treated fields. Ladybird beetles, syrphid flies, hymenopteran wasps, and parasitism rate of R. pedestris eggs were not different between the treatment and control. These results suggest that flonicamid and B. bassiana showed less negative effect on the natural enemies and effective against aphids and nymphs stage of R. pedestris.