The objective of this study was evaluated the influences of the nitrogen fertilization level on timothy silage fermentation quality, nutritive value, and nutrient intake. In both the first and second crops, high level nitrogen fertilization (H) led to a lower WSC content and higher CP content than standard nitrogen fertilization (S). The silage fermentation quality was good in the presence of a Lactobacillus inoculant (SI and HI) than uninoculant (SC and HC). In the first crop silage, the TDN content and intake were significantly higher (P＜0.01) for HI than for SI. In the second silage, the DM, DE, and TDN intakes did not significantly differ among the 4 treatments.