검색결과

검색조건
좁혀보기
검색필터
결과 내 재검색

간행물

    분야

      발행연도

      -

        검색결과 31

        1.
        2023.10 구독 인증기관·개인회원 무료
        The walking-stick insects, Ramulus mikado, are outbreaks in several mountainous areas from 2020 to 2022. In recent, some population of the insect are showed rapidly decline in their abundance, while some of them are still maintained high population density. In worldwide, insects belonging to Phasmida are reported to outbreak in their habitats as mentioned above environments, but knowledge about outbreak pattern of the walking-stick insects is still lacking. In this study, we aimed which biological and environmental factors are related to wax and wane of the insect population. From 2022 to 2023, we studied host tree preferences in natural conditions, ecological stoichiometry in major host trees, overwintering ecology of R. mikado eggs, and infection rate by entomophathogenic fungi during growing season.
        3.
        2022.10 구독 인증기관·개인회원 무료
        Investigations and monitoring of environmental radiation are important for preventing expected accidents or for early detection of unexpected accidents, in nuclear facilities and the surrounding. In the event of an environmental radiation accident, it should be possible to identify and analyze the radiation-contaminated area. Therefore, a rapid radiation monitoring system is required for immediate response and necessary measures. In this study, the distribution of radiation mapping is performed on a contaminated area using 2-dimensional or 3-dimensional contour mapping techniques. The entire surrounding area can be understood at a glance by displaying the radiation contour line on the map of the measured area.
        6.
        2018.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Musculoskeletal disorders including fracture, tendonitis, osteoarthritis, and laminitis are common diseases in racehorses that can cause large economic losses in the racehorse industry. Mesenchymal stem cells (MSCs) are being applied as new clinical tools for treatment of musculoskeletal disorders of racehorses. To investigate the immunomodulatory effects of stem cell therapy, we analyzed the anti- and pro-inflammatory factors in peripheral blood mononuclear cells of racehorses before and after stem cell application using quantitative real-time RT-PCR. The expression levels of pro-inflammatory factors (CCL5, IFN-γ, IL-2, and IL-18) were decreased while those of anti-inflammatory factors (TIMP-1, IL-10, TGF-β1, and VEGF) were increased significantly after application of equine adipose tissue-derived MSCs (eAD-MSCs) to racehorses with fracture. Moreover, the expression levels of pro-inflammatory factors (IL-2, IL-18, and TNF-α) were decreased while those of anti-inflammatory factors (TIMP-1, TIMP-2, IL-10, TGF-β1, and VEGF) increased significantly after stem cell application of eAD-MSCs in racehorses with tendonitis. After evaluating immunomodulatory effects of stem cell therapy on equine musculoskeletal disorders such as fracture and tendonitis, our results showed that expression levels of pro-inflammatory factors were decreased, while those of anti-inflammatory factors increased significantly after stem cell application of eAD-MSCs. These findings suggest that the healing effects of the stem cell therapy might be due to its modulation of inflammatory factors.
        4,000원
        7.
        2018.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mesenchymal stem cells (MSCs), which are present in all tissues, can differentiate into cells with various specific functions. Recently, cell-based therapies using MSCs have been increasing in the veterinary research and related fields. In this study, we investigated the cellular morphology, proliferating capacities, expression of cell surface markers such as CD13, CD34, CD44, CD45, CD90, and CD105, mesodermal differentiation potentials, and expression of senescence-related markers of p53, p21, and telomerase reverse transcriptase in equine adipose tissue-derived MSCs (eAD-MSCs) after cryopreservation. The eAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 1 year (< 1 year, G1) and more than 3 years (> 3 years, G2), respectively. After cryopreservation for 1 - 3 years, G2 eAD-MSCs showed similar cellular morphology, proliferating capacities, and expression of cell surface markers when compared with G1 eAD-MSCs. Moreover, cryopreservation did not affect the adipogenic, chondrogenic, or osteogenic differentiation potentials of G1 and G2 eAD-MSCs. Collectively, cryopreservation for (or over) 3 years maintained the stem cell phenotype and differentiation potentials of eAD-MSCs. These results will be an advantage that can be effectively used for future development of cell-based therapies.
        4,000원
        8.
        2018.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The important PH (paleontological heritages) with scientific, educational and esthetic values designated as natural monuments and protected by legislations of South and North Koreas are herein compared for the first time. On the basis of data (Jan. 2017) provided by the Cultural Heritage Administration of (South) Korea, a total of 457 natural monuments was designated. Of these, geological heritages are 80 in number, which includes 24 (30%) PH. Data (Dec. 2005) of North Korea show that a total of 474 natural monuments was designated. Among these, geological and geographical ones are 154 in number, which includes 22 (14%) PH. Differences between PH of South and North Koreas are regarded to be directly related with geological difference in distribution of the fossil-bearing strata between South and North Koreas. PH of Silurian corals, Devonian plants, Jurassic fishes, Cretaceous dinosaur tracks, birds (so called Korean Archaeopteryx) and pterosaurs, and Pleistocene paleoanthropological fossils appear to be scientifically significant. Together with these North Korean PH, scientific, esthetic, conservational, educational, and economical values of important PH including KCDC (Korean Cretaceous Dinosaur Coast), Jigunsan Shale, and Geumgwangdong Shale of South Korea should be evaluated as potential future candidates for international heritages.
        5,800원
        9.
        2017.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Induced pluripotent stem cells (iPSCs) can be generated from adult cells. Somatic cells can be reprogrammed to form iPSCs by overexpressing transcription factors such as Oct4, Sox2, cMyc, and Klf4. To maintain undifferentiated state of iPSCs in vitro, cells have traditionally been maintained on mouse embryonic fibroblast feeders and passaged by enzymatic or mechanical dissociation methods. In this study, we compared the morphology and pluripotency of porcine iPSCs (piPSCs) after subsequent passaging using enzymatic and mechanical dissociation methods. Enzymatically and mechanically passaged piPSCs showed embryonic stem cell-like morphologies with compact cell adhesion and clear colony borders. In addition, alkaline phosphatase staining was positive for both enzymatically and mechanically passaged piPSCs. However, visual observation revealed that some colonies of enzymatically passaged piPSCs were spontaneously differentiated more than those of piPSCs mechanically passaged from 5 passage. Quantitative real-time RT-PCR demonstrated that enzymatically and mechanically passaged piPSCs expressed pluripotent genes such as Oct4, Sox2 and Nanog well at early passage. Immunofluorescent staining also confirmed that pluripotent markers such as Oct4, Sox2, and Nanog were positively expressed at early passage. However, expression levels of pluripotent genes in mechanically passaged piPSCs were also higher than those in enzymatically passaged piPSCs at early passage. Collectively, we found that mechanical passage method was better than enzymatic passage in terms of morphology and pluripotency of piPSCs at early passage. Further studies are needed to compare these dissociation methods with those obtained after more passages of piPSCs.
        4,000원
        10.
        2017.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mesenchymal stem cells (MSCs) have restricted life spans in vitro and can therefore only be expanded for a limited number of cell divisions before entering a senescent state and unequivocally stopping proliferation. Several types of cell culture systems have been used for large-scale expansion of MSCs. A recent trend in cell culture has been the change from serum-use to serum-supplement media. This study was conducted to investigate the proliferative effects of vegetable resources (VR) on equine adipose tissue-derived mesenchymal stem cells (eAD-MSCs) in the absence of serum and their possible mechanisms of action. Regulation of cell cycling is a key process involved in the fate of stem cells, including renewal and differentiation. In this study, we observed that the viability of eAD-MSCs was increased significantly when treated with VR under serum-free conditions. We also observed that expression levels of cell cycling-related proteins such as p53 and p21 were decreased, and proliferating cell nuclear antigen increased significantly in response to treatment with VR in eAD-MSCs under serum-free conditions. Furthermore, expression levels of cell survival-related proteins were increased in response to treatment with VR in eAD-MSCs under serum-free conditions. Therefore, our results suggest that VR promotes proliferation of eAD-MSCs under serum-free conditions.
        4,000원
        11.
        2017.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several cell lineages, which has implications for cell therapy and reproductive biotechnologies. Although MSCs have been isolated from many species, including humans and animals, there is limited data on MSCs from large ruminants, such as bovines. In this study, we tried to isolate and characterize bovine tongue tissue-derived MSCs (boT-MSCs) by investigating phenotype morphology, performing proliferation properties, and determining cell surface marker expression patterns, self-renewal, and differentiation potentials. As a result, the boT-MSCs were successfully isolated by collagenase digestion and maintained proliferative capacity until 20 passages. Moreover, the boT-MSCs expressed pluripotency markers (OCT3/4, SOX2, and NANOG) and MSC-specific surface markers including CD44, CD90, and CD105, but not CD45 and MHC-II. The boT-MSCs could also differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Our results suggest that the tongues of bovines could be used as a source of MSCs.
        4,000원
        12.
        2016.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mesenchymal stem cells (MSCs) are primary candidates for cell therapy and tissue engineering applications. A two-dimensional (2D) culture system is typically used for cell growth, but that method affects the characteristics of stem cells. The physiological cell environment connects cells not only to each other, but also to the extracellular matrix providing mechanical support, exposing the entire cell surface, and opening signaling pathways. The hanging drop method is the most widely used 3D culture method for spheroid formation. In this study, we investigated the relationship between spheroid size and changes in gene expression to determine the optimum spheroid size for use in tissue engineering. The expression levels of stemness factors such as NANOG, OCT4, and SOX2, angiogenic factors such as VEGF and IL-8, and osteogenic factors such as COX2 and TGF-β1 increased with spheroid size in the respective spheroid formation groups unlike the responses in their monolayer groups. Therefore, our results indicate that spheroid formation through the hanging drop method can increase the efficiency of MSCs-based tissue engineering over that obtained via traditional 2D cell culture systems.
        4,000원
        13.
        2016.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mesenchymal stem cells (MSCs) have been researched for use in biomedical applications, particularly for cell-based therapies and regenerative medicine due to their self-renewing capacity and ability to differentiate into multiple cell types such as adipose, bone, and tendon tissues. Cryopreservation of MSCs is a common preservation method that is advantageous for cellular therapies in human and veterinary medicine. Adipose tissue-derived cells have been shown to maintain their properties after cryopreservation. In this study, we investigated the morphology, proliferation (cumulative population doubling level and doubling time), cell surface markers (CD34, CD90, and CD105), and ability to differentiate into adipose, bone, and cartilage tissues in vitro of equine adipose tissue-derived MSCs (eAD-MSCs) and miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with and without long-term cryopreservation. The eAD-MSCs and mpAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 3 years and 2 years, respectively. Cryopreserved eAD-MSCs maintained their morphology, proliferation rate, and cell surface markers compared with fresh cells. With the exception of proliferation rate, cryopreserved mpAD-MSCs also maintained their fresh cell characteristics. The proliferation rate of cryopreserved mpAD-MSCs was higher than that for fresh cells. Cryopreservation did not change the adipogenic, chondrogenic, or osteogenic differentiation potentials of eAD-MSCs and mpAD-MSCs. In summary, long-term cryopreservation maintains the cell phenotype and differentiation ability of eAD-MSCs and mpAD-MSCs. These results might be useful when developing veterinary medicine and clinical applications.
        4,000원
        15.
        2015.11 구독 인증기관·개인회원 무료
        A novel cation exchange membrane consisting of polyvinylidene difluoride (PVDF) was prepared for the application of vanadium redox flow battery (VRFB). PVDF used as supporter has considerably high mechanical strength and an intrinsic hydrophobicity. For the successful preparation of the membrane, PVDF powders were modified by potassium hydroxide, which increased the hydrophilicity of PVDF powders. Modified PVDF were grafted with styrene sulfonic acid (SSA) using benzoyl peroxide (BPO) as initiative. The cross-sectional morphology and structure of PVDF/SSA was confirmed by scanning electron microscopy (SEM) and FT-IR. The membrane was characterized by water uptake, dimensional change, ion conductivity and ion exchange capacity (IEC) and cell performance of Vanadium Redox Flow Battery (VRFB) with Nafion 212.
        16.
        2015.10 구독 인증기관·개인회원 무료
        Athetis dissimilis (Hampson) (Lepidoptera: Noctuidae) [뒷흰날개담색밤나방] was attracted to pheromone traps for Euzophera batangesis (Lepidoprera Pyrimidae) [밤알락명나방], when occurrence of E. batangesis was monitored in sweet persimmon orchard with its pheromone, (Z)-9-tetradecenol (Z9-14OH), (Z9,E12)-9,12-teradecadienol (Z9,E12-14OH) and their mixture. With the components, we monitored the seasonal occurrence of A. dissimilis, tested response of the moth to single or mixed components, and compared efficacy of three different types of traps. A. dissimilis was trapped on May 14 for the first time. First occurrence peak was observed during June 4-24 and second peak during August 20 – September 10. A. dissimilis was attracted to single component of Z9-14OH and Z9,E12-14OH and to their mixture at Jinju, Sancheong, and Sacheon. In trap efficacy tests, delta traps with white color caught most number of the moths significantly at Sacheon. However, at Sancheong, there was no statistical difference among the traps, although funnel trap caught most number of A. dissimilis. Pest status of A. dissimilis is not known eg. whether it is a pest of persimmon or other fruits. However, with Z9-14OH, E9,E12-14OH and their mixture, it is expected to be monitored and controlled.
        17.
        2015.10 구독 인증기관·개인회원 무료
        Rapid growth in trade of agricultural products has increased the risk of introduction of exotic pests into new area. In order to fulfill quarantine obligations, several treatments are in practice: fumigation with methyl bromide (MB), application of extreme temperatures, controlled atmospheres, and combinations of them. Although MB was most effective and widely applied for quarantine treatment, its use was banned by the Montreal Protocol. Ionizing irradiation phytosanitary treatments – gamma radiation, electron beam, and X-ray are being used as an alternative to MB currently. For applying ionizing irradiation as a quarantine treatment, there is a research protocol which should be followed, International Standards for Phytosanitary Measure 18 [ISPM #18] and Phytosanitary Treatment Regulation of Korea (Notice 2014-35). We have evaluated the effect of gamma radiation, electron beam, and X-ray on H. armigera as quarantine treatments. In this presentation, ISPM #18 and Notice 2014-35 will be talked with the example of H. armigera.
        18.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted from October 2014 to May 2015 to explore forage production and feed values of Italian ryegrass, Rye and whole crop barley as winter forage crops in the Southern region of Korea. The experimental location was over 10 points for each species and each sampling point area was 1 m² (Width: 1 m × Length: 1 m). Air mean temperature and rainfall in the Southern region of Korea during the experimental period was 6.95 ± 5.75℃ and 70.45 ± 54.68 mm, respectively. Fresh forage yield of Italian ryegrass, the most cultivated forage in the Southern region of Korea, was 44.4 ± 7.0 ton/ha. The percentage of dry matter for whole crop barley was 28.9 ± 7.0%. Crude protein (CP) was higher in Italian ryegrass (10.7 ± 5.3%) while total digestible nutrient (TDN) had the highest value in whole crop barley. Crude protein was not significantly different by location. However, the neutral detergent fiber (NDF), acid detergent fiber (ADF) and total digestible nutrient value of forage from Jeonbuk province were higher than in forage from Gyeongnam province.
        4,000원
        19.
        2014.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study attempted to decompose typical odorous VOCs including toluene, isopropyl alcohol, benzene and acetone using a dielectric barrier discharge plasma in a pilot experiment scale up to 60 m3/min. It utilized oxygen ions(205,000 ions/cm3) and various radicals formed by a plasma generator. Although ozone as a byproduct( approximately 300 ppb) contributes to destroying organic compounds in their gas phase, the residual release could be captured by post manganese filters showing a maximum 30% removal. The present process was more effective for acetone, which is composed of relatively simple molecules, with a 56% decomposition rate. Whilst toluene, benzene and isopropyl alcohol were shown to have of rates of 19%, 37% and 36% respectively.
        4,000원
        20.
        1988.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Five nematophagous fungi were evaluated for their nematicidal effect in vitro on Rhabditis sp. and Meloidogyne hapla in soil. Inocula of Arthrobotrys arthrobotryoides, A. conoides, A. oligospora, Dactylella lobata, and Fusarium oxyaporum were grown in moistened corn-sandy soil and chopped potato-sandy soil media, and incubated at 26 for one week. The prepared inocula were incorporated in autoclaved sandy soil, mixing thoroughly at rates equ-invalent to 1:50, 1:100, 1:200, and 1:400, repectively, before 80g of the mixture carrying 100 Rhabditis sp. was put into petri plates. Nematophagous fungi effectively teduced the popuation of Rhabditis sp. in soil in a week or two following treatment of the incula at concentration of 1:50 and 1:100. The optimum was at for nematicidial effect as high as 80-100%. The at the rate of 1:100 prepared incula were incorporated in auto-claved soil, where 100 Juveniles M. hapla were introduced per 80% soil. All fungi infected the M. hapla effectively in soil, caysing more than 90% mortality within one week. This result indicated the potential value of these fungi as promising biocontrol agents
        4,000원
        1 2