The organism evaluation equipment (OEE) was developed to determine the insecticidal effect of atmospheric-pressure plasma jets (APPJ). The equipment consisted of a plasma-generating acrylic chamber (PGAC) equipped with a plasma generator, a plasma-maintaining acrylic chamber (PMAC), and plasma efficacy evaluation container (PEEC). The amount of reactive oxygen species (ROS) directly exposed to PEEC in the PMAC was measured using an ozone calculator. The ROS concentration in the PGAC and PEEC increased over time, and the optimum position for the PEEC was selected showing the lowest variance of ROS concentration. Based on the established equipment, five major insect pests (Aphis gossypii, Bemisia tabaci, Helicoverpa armigera, Tetranychus kanzawai, and Thrips palmi) were tested, and their knockdown time, recovery time, and median lethal time (LT50) were determined. As a result, B. tabaci showed the fastest knockdown in respond to the plasma, but the remaining insect pests were knock downed within 2-3 min. Recovery was the fastest in T. palmi and T. kanzawai and slowest in B. tabaci. The LT50 were determined as 13 and 16 min in B. tabaci and T. palmi, respectively. However, the LT50 of other insect pests showed over 21 min. In conclusion, the OEE system can be used to measure the optimum exposure time of plasma against various insect pests, and the plasma might be used as an alternative tools for pest control in the future.