This study was conducted to improve the quantity of fatty acid in peanut oil for quality breeding program. To determine fatty acids of oleic acid and linoleic acid in F2 generation, we used near infrared reflectance spectroscopy (NIRS) with high R2 values of 0.983 and 0.991 for oleic and linoleic acids, respectively. For this experiment, two combinations with 2 mother plant( cultivar Sangpyeong and Baekseon) and 1 father plant (YGC 2) were analysed. Each cultivar Sangpyeong, Baekseon, and YGC-2 in oleic and linoleic acid have 41.6%, 58.8%, 76.9% and 44.9%, 26.2%, 5.7% respectively. Distribution of fatty acid in combination Sangpyeon/YGC-2 in oleic acid showed 38.2% to 83.5%, and in linoleic acid showed 43.2% to 6.3%. The combination of Baekseon/YGC2-2 also showed 44.6% to 81.0% in oleic acid, and 40.1% to 6.8%. This result showed the values of fatty acid in F2 generation had more wide continuous variation, compared with parents values.