Changes in contents of free sugars, amino acids, and fatty acids of legumes were analyzed for each phase of in vitro digestion. In addition, contents of resistant starch in raw and digested pulses were compared. Soybeans, kidney beans, cowpeas, and chickpeas were analyzed. An in vitro digestion model was used to analyze contents of nutrients using LC-MS and GC-MS. Stachyose in kidneybean, cowpea, and chickpea increased as the digestion phase progressed. In four types of legumes, raffinose slightly decreased or showed no significant difference between the Oral phase and the BBMV phase. Content of glucose, a monosaccharide, increased during the BBMV phase. During the digestion phase, levels of free amino acids and free fatty acids also increased. Content of resistant starch was reduced compared to that in the raw material. It was 0.01g/100 g food in soybean, 1.06 g/100 g food in red kidney bean, 0.77g/ 100g food in cowpea, and 0.76 g/100 g food in chickpea. It was confirmed that nutrients in the in vitro digestion model were liberated at each digestion phase with changes in the content of resistant starch. These results are expected to be used as fundamental data for obtaining bioavailability of nutrients.

In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6''-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6''-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

The pre-treatment conditions of butterbur stem such as blanching, freezing, and thawing were studied to optimize it for producing frozen butterbur stem to improve its storage ability. Butterbur stems were hot water blanched at 100oC for 3, 5, or 7 min respectively and then soaked in cold water. After peeling out and cutting into blocks (4.5×1.0×0.5 cm), butterbur stems were air-dried for 5, 10, or 15 min respectively. Dried samples were analyzed for their physicochemical properties. With blanching, the hardness value increased from 2.91 kg to 3.64 kg (p>0.05); however, adhesiveness decreased drastically after 5 min of blanching (p<0.05), which changed to a crisp texture. In addition, considering other physicochemical properties, it was assumed that 5-min blanching was optimal pre-treatment to maintain the original quality of butterbur stem for freezing. Five-minute blanched butterbur stems were airdried 5 min, frozen and thawed with several methods, and analyzed for their properties. Considering the thawing loss, hardness, and color change, the fastest freezing and thawing method had the lowest changes on the quality of frozen butterbur stem. Therefore, to produce frozen butterbur stem, it was assumed that immersion freezing and running water or room temperature thawing (25oC) were the best process.

본 연구에서는 수문사설 에 수록된 토란병의 제조방법을 확립하기 위하여 토란의 가열 시간 및 고명에 따라 토란병을 제조하고, 품질특성을 비교․분석하였다. 가열 시간에 따른 수분, 조단백질, 조지방 및 조회분 함량은 각각 83.42~84.61%, 1.25~1.31%, 0.08~0.11%, 0.62~0.81% 범위로 조지방은 유의적 인 차이가 없었으며, 조회분은 가열처리로 인해 유의적으로 감소하였다. 토란의 주요 유리당은 fructose, glucose 및 sucrose 가 검출되었고, 가열 시간에 따라 각각 0.17~0.33%, 0.16~0.29% 및 0.26~0.38% 범위로 15분 가열처리 때까지 유의적으로 감 소하였다. 경도 역시 가열 시간이 늘어날수록 2.75 ㎏에서 0.14 ㎏까지 감소하였다. 가열 시간에 따른 관능특성을 평가한 결 과, 향과 맛은 각 처리구간 유의적인 차이를 보이지 않았고, 질감과 전반적인 기호도는 5분 처리구가 다른 처리구에 비해 유의적으로 낮게 평가되었으며, 10분 처리 이후에는 큰 차이 를 보이지 않았다. 이상의 결과, 토란병의 제조 시 가열 시간 은 10분이 바람직할 것으로 판단된다. 잣고물, 콩고물, 검은 깨고물 및 밤고물 등 고명 종류에 따른 토란병의 관능특성을 평가한 결과, 색, 향, 질감, 맛 및 전반적인 기호도는 각각 5.20~7.50, 5.40~7.70, 6.10~7.00, 6.10~7.20 및 6.20~7.60 범위 로 평가되었고, 콩고물을 묻힌 토란병이 모든 항목에서 가장 높게 평가되었다.

본 연구에서는 열처리가 야콘의 항산화 활성에 미치는 영향을 살펴보기 위하여 열처리 온도(100 및 121℃)와 시간 (15, 30 및 60분)에 따른 항산화 성분 및 항산화 활성 변화를 조사하였다. 열처리 후 야콘의 갈변도, 유리형 및 결합형 폴리페놀 함량, 유리형 및 결합형 플라보노이드 함량, DPPH radical 소거 활성 및 ABTS radical 소거 활성을 측정하였 다. 열처리 후 야콘의 갈변도, 유리형 폴리페놀 및 플라보노이드 함량과 항산화 활성은 열처리 온도와 시간에 따라 유의적(p<0.05)으로 증가하였고, 결합형 폴리페놀 및 플라보노이드 함량은 감소하였다. 야콘의 유리형 폴리페놀 및 플라보노이드 함량은 121℃, 60분 열처리 시 생야콘에 비해 각각 1.2배 및 1.1배로 유의적(p<0.05)으로 증가하였다. 또한, 야콘의 DPPH radical 소거 활성 및 ABTS Radical 소거 활성도 121℃, 60분 열처리 시 생야콘에 비해 각각 1.7배 및 2.0배로 유의적(p<0.05)으로 증가였다. 야콘의 갈변도, 폴리페놀 및 플라보노이드 함량과 DPPH radical 및 ABTS radical 소거 활성 간의 상관관계를 분석한 결과, 높은 상관관계(p<0.01)가 있는 것으로 나타났다. 본 연구결과, 열처리 방법을 통해 야콘의 항산화 성분과 항산화 활성을 강화시킬 수 있으며, 이를 활용한 기능성 식품 소재 개발이 가능할 것으로 생각된다.

국내산 녹두전분과 도토리전분을 이용하여 초산 녹두 및 초산 도토리전분을 제조하고, 이화학적 특성 및 겔의 조직감을 비교하였다. 무수 초산 6%를 반응시켜 제조한 초산 녹두와 초산 도토리전분의 %acetyl 함량은 1.88%과 1.53% 였고, 치환도는 0.07과 0.06이었다. 전분의 색도를 측정한 결과, L값과 a값은 증가하였고, b값은 감소하여 초산 처리가 전분의 색에도 영향을 주었음을 알 수 있었다. 또한 초 산전분은 전분 chain 내부에 초산기가 도입되어 원료 전분 에 비해 blue value, 팽윤력 및 용해도가 증가하는 것을 알 수 있었다. 신속호화점도계(RVA)를 이용하여 측정한 호화 특성은 녹두전분과 도토리전분의 호화온도가 각각 70.4oC, 75.7oC로 나타났고, 초산 녹두 및 초산 도토리전분은 각각64.4oC, 74.8oC로 나타나 초산 처리 시 호화온도가 감소하는 것을 알 수 있었으나, 도토리전분의 경우 초산 도토리 전분과의 유의적인 차이는 나타나지 않았다. 초산전분은 원료 전분과 최고점도, setback, breakdown에도 차이를 보였는데, 초산 녹두전분은 최고점도 164.9 RVU, setback 70.9 및 breakdown 53.4로 녹두전분의 최고점도 195.0 RVU, setback 110.6 및 breakdown 69.0에 비해 감소하였고, 초산 도토리전분은 최고점도 212.1 RVU, setback 128.7, 및 breakdown 50.3으로 도토리전분의 최고점도 198.3 RVU, setback 87.0 및 breakdown 38.2보다 증가하는 특성을 나타내었다. 초산전분으로 겔을 제조하여 조직감을 측정한 결과 초산 녹두전분은 겔을 형성하지 못하였고, 초산 도토리전분은 원료 전분 겔에 비해 경도와 탄성이 감소하였으며, 응집성, 검성, 씹힘성이 증가하여 겔이 무른 특성을 나타내었다.

The purpose of this study is to determine the possibility of using cultured wild ginseng roots as a natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of cultured wild ginseng roots were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 61.72%, 17.36%, 0.23% and 10.90%, respectively. Further, the calories of cultured wild ginseng roots were 323.97 kcal. Total dietary fiber was 82.13%. The protein contained a total of 18 different kinds of amino acids. The contents of amino acids were 16.15 g. The K was the largest mineral followed by P, Ca, and Mg, which means cultured wild ginseng roots is alkali material. The contents of saturated fatty acids and unsaturated fatty acids were 0.23 g, and 0.62 g, respectively. Crude saponine content was 25.87 ㎎/g. Total phenolic contents of cultured wild ginseng roots were 11.2 ㎎/g, and total flavonoids contents were estimated as 4.2 ㎎/g. The electron donating ability of cultured wild ginseng roots were 24.7～31.6%. The nitrite scavenging activity was pH dependent, and was highest at pH 1.2 and lowest at pH 6.0. The cultured wild ginseng roots extract showed the highest reducing power (0.06) at the concentration of 1, 000 ㎍/㎖. Based on the above results, we deemed that the cultured wild ginseng roots might have potential antioxdant activities.

We conducted this study to investigate the quality characteristics of mashed red pepper(MRP) with different freezing(－20, －30, －40, －50, －60 or －70℃) and thawing(4, 10, 20, 30, 40 or 50℃) temperature. Frozen and thawed MRP was evaluated for ascorbic acid contents(AsA), capsaicinoids contents, free sugar contents, ASTA value, and flavor patterns. The AsA of frozen MRP with freezing temperature showed a range of 67.08～80.35 ㎎/100 g FW, and the mean AsA losses after frozen were 57～64% compared to raw red pepper. Capsaicinoids contents, free sugar contents, and ASTA values of frozen MRP with freezing temperature were no significant difference compared to raw red pepper. The AsA of thawed MRP with thawing temperature showed a range of 70.34～81.59 ㎎/100 g FW, and the mean AsA losses after thawed were 45.12～52.69% compared to raw red pepper. Capsaicinoids contents and free sugar contents of thawed MRP with thawing temperature were no significant difference compared to raw red pepper, whereas the ASTA value decreased according to the increasing of thawing temperature. Flavor patterns of thawed MRP after 20～50℃ thawing were clearly different from the raw red pepper. Overall, it is recommended that the best freezing and thawing temperature for preserving the quality of MRP is freezing at －20℃ and thawing at 10℃.

In this study, we investigated the growth of striped mullet (Mugil cephalus), Yesso scallop (Patinopecten yessoensis) and kelp (Saccharina japonica) farmed under the IMTA (integrated multi-trophic aquaculture) system developed by national institute of fisheries science (NIFS). The farmed striped mullets grew from an initial length and weight of 152.5±12.1 and 41.6±7.8 g in October 2013 to 154.2±5.6 and 47.5±8.6 g in November, 160.2±8.7 and 55.9±9.1 g in December and 168.4±9.6 and 58.4±8.7 g in January. The fish continued to grow and reached 190.2±9.4 in length and 87.5±8.9 g in weight in April and 256.4±9.7 and 156.7±6.7 g in October 2014. The daily growth rate (DGR) for total fish length was 0.015 0.1 /day during the periods of fast growth and attained 0.038 0.1 /day during February March. The kelp grew from an initial blade length and wet weight of 1.19±0.2 and 0.0028±0.0012 g in January 2014 to 3.3±0.8 and 2.5±0.9 g in February and 126.5±11.6 and 107.4±22.6 g in March, after which, erosion occurred and slowed the growth. The DGRs for kelp length ranged 0.03 1.9 /day in January 2014 and increased to 0.88 1.9 /day during March April. Increasing water temperatures beginning in April lowered the DGR to 0.03 /day. Yesso scallops grew from an initial shell length, shell height and wet weight of 11.83±0.6 , 12.68±0.7 and in September 2013 to 19.9±2.5 , 20.8±2.6 and 0.9±0.04 g in November 2013. They continued to grow to 45.91±0.71 in shell length, 42.55±0.8 in shell height and 12.7±1.3 g in wet weight by May 2014 and 60.2±2.51 , 554.6±2.61 and 24±2.70 g by October 2014. The DGRs for shell length of Yesso scallop ranged from 0.02 to 0.256 /day with higher values of 0.256 0.27 /day during November December 2013 and March April 2014.

마늘의 냉동에 따른 생리활성을 변화를 분석함으로서 생리활성 효능이 유지되는 최적의 냉동 온도를 선정하고자 냉동조건에 따른 마늘 추출물의 항산화 및 항균활성을 비교 분석하였다. 마늘 추출물의 페놀 화합물 함량은 6.91±0.69 μg GAE/g으로 냉동 조건(-20, -80, -196℃)에 따라 큰 차이를 보이지 않았으나, 플라보노이드 함량은 -80℃에서 0.327±0.058 μg QE/g에 반해 -20℃ 에서 0.263±0.015 μg QE/g으로 소폭 감소를 나타내었다. DPPH 라디칼 소거능 분석에서는 2 mg/mL의 추출물에서 4℃와 -80℃에서 61%로 나타난 것에 비해 -20℃와 -196℃에서는 51%가량으로 10%정도 라디칼 소거능이 감소하는 경향을 나타내었고, 마늘 추출물의 SOD 유사활성은 4℃ 대조군에 비해 냉동조건에서 전반적으로 증가 경향을 나타냈다. 아질산염 소거능은 pH 3.0 조건에서 18% 수준에서 소폭 증가가 나타난 반면 환원력 비교에서는 700 nm의 흡광도가 1.6 abs 수준에서 냉동조건에 따라 감소하는 경향을 나타내었고 -80℃에서 감소폭이 가장 적게 나타났으나 유의적인 수준은 못 되었다. 항균활성 비교 실험에서는 마늘 추출물이 E. coli, S. typhimurium, V. vulnificus, S. aureus와B. cereus에 대해 높은 항균활성을 나타냈으며, 마늘의 냉동 조건에 따라서는 유의적 차이를 보이지 않았다. 이상의 결과를 종합하여 볼 때 마늘의 냉동 후 추출물의 생리활성은 실험조건에 따른 변화 폭이 크지는 않지만 페놀 화합물과 플라보노이드 및 이에 의한 항산화 및 항균 활성이 안정적으로 유지되는 최적 온도는 -80℃로서, 마늘의 냉동 저장 중 품질특성 뿐만 아니라 생리활성기능 유지라는 두 가지 측면에서 -20℃ 관행 냉동보다는 저온급속냉동이 필요하다고 판단된다.