The Varroa mite, Varroa destructor, a parasitic mite that afflicts honey bees, has become increasingly resistant to acaricides like fluvalinate due to its widespread use. The target site insensitivity mechanism, mediated by the L925V/M/I mutations in the voltage-gated sodium channel, plays a major role in resistance. Additionally, cytochrome P450 monooxygenases (Cyp450s) appear to function as a metabolic resistance factor; however, no Cyp450-mediated resistance mechanism has been reported to date. The aim of this study was to identify and characterize Cyp450s associated with fluvalinate resistance. A synergistic bioassay confirmed the involvement of Cyp450s in conferring tolerance or resistance to fluvalinate. Correlation analysis between mortality data and the expression levels of Cyp450 genes led to the identification of several candidates that may play a crucial role in fluvalinate resistance. Analysis of tissue distribution patterns revealed that these genes were most abundantly expressed in the cuticle and synganglion. This suggests that, despite their relatively low expression level, they may play a critical role in protecting the target site from fluvalinate due to its predominant expression in neuronal tissues. Functional analysis, in conjunction with baculovirus expression, demonstrated that fluvalinate has high inhibition rates against the recombinant candidate Cyp450s, suggestive of their strong interaction with fluvalinate. We discussed the potential utilization of their expression levels as a molecular marker for diagnosing metabolic resistance in field-collected Varroa mites.

A 13-year-old mixed dog was referred to the animal medical center, Gyeongsang National University. Lung masses were diagnosed at the left cranial and caudal lobes through diagnostic imaging, and consequently, left pneumonectomy was performed using a self-cutting linear endoscopic stapler. The pulmonary arteries, veins, and bronchus of each lung lobe were sealed and resected at once, and any air leakage or bleeding was not observed after the surgery. Compared to the conventional ligation method, the self-cutting linear endoscopic stapler has the advantage of significantly reducing the operation time and enabling simple and reliable sealing.

본 연구는 국내 자생하는 진달래속(Rhododendron) 종들의 종자 휴면유형 분류 및 발아특성 구명을 목표로 하였다. 진달래속 종들의 배는 형태적휴면(MD)이 없는 완전히 발달된 직선(linear) 형태였으며, 만병초 및 꼬리진달래 종자는 탈리 시점에 이미 휴면이 없는 것으로 밝혀졌다. 반면에 털진달래 종자는 population 수준에서 부분적으로 생리적휴면(PD)을 가지고 있는 것으로 나타났다. 털진달래의 이러한 생리적휴면 (PD)은 외생 지베렐린(GA3) 1,000mg･L-1 처리를 통해 타파될 수 있었다. 그러나 4℃에서의 저온층적처리는 털진달래 종자 휴면 타파에 효과가 없는 것으로 나타났다. 종합적으로 판단 했을 때, 진달래속(Rhododendron) 종들의 적정 발아 환경조 건은 광조건･25/15℃(만병초), 암조건･20/10℃(꼬리진달래), 광 조건･25/15℃(털진달래)로 확인된다. 진달래속(Rhododendron) 에서의 종간 차이(interspecific variation)로 인해 모든 종이 종자 휴면유형 또는 발아특성에서 구별이 되었다. 본 연구는 국내 자생하는 진달래속(Rhododendron) 종들의 생리･생태 특성을 이해하는 데 이용될 수 있을 것이다.

본 연구의 목적은 개폐식 대공간 구조물의 풍하중 산정 및 구조해석의 과정을 자동으로 수행하는 컴포넌트를 개발하는 것이다. 설계한 파라메트릭 모델링을 StrAuto를 통해 구조해석 자동화단계를 거쳐 구조해석용 모델로 변환하는 과정을 실시간 으로 연동하여 구조해석 결과를 자동으로 도출하는 과정으로부터 본 연구에서는 추가로 구조물의 풍하중을 형상에 따라 상세히 할당하는 기능을 개발하였다. 이와 같은 과정을 통해 풍하중에 대한 최적화를 수행하여, 기존 설계된 구조의 물량을 줄이고, 구조적 안정성은 유지하는 방향으로 결론을 도출하였다. 추후에는 본 예제 모델을 통해 진동제어 최적화를 위한 제진 장치 설치위치의 자동탐색이 가능하게 되는 연구를 진행할 계획이다.

To identify and compare the venom components and expression patterns of some bees/wasps, venom gland-specific transcriptome analyses were conducted for 14 Aculeate bees/wasps. Most of the allergens and pain-producing factors showed extremely high expression levels in social wasps, implying that social wasps have evolved to use venom to defend the colony against intruders. Acid phosphatase and tachykinin, which are known as allergens and neurotoxic peptides, were found with high frequencies in the venom glands of solitary wasps. This suggests that solitary wasps might use their venom for catching and preserving prey. In the venom glands of bumblebees, little or no transcripts of major allergens or pain producing factors were identified, implying that bumblebees venoms are relatively less toxic than those of social or solitary wasps. Taken together, the differential expression patterns of venom genes in some Aculeate bees/wasps implies that bees/wasps have unique groups of highly expressed venom components, which appear to have evolved in response to both ecological and behavioral influences.

Mosquitoes are primary medical insect pests due to their diseases transmission as vectors. In Korea, the insecticide-resistant populations of disease vector mosquito species, such as Anopheles sinensis, Culex pipiens and Culex tritaeniorhynchus, have constantly increased. Thus, management of insecticide resistance to major insecticides including pyrethroids and organophosphates is required for more efficient control of resistant populations. In this study, the quantitative sequencing (QS) protocols were established to detect the frequencies of three mutations (the L1014F on voltage sensitive sodium channel and the G119S and F331W on acetylcholinesterase 1) that are associated with either pyrethroids or organophosphates. Based on the QS protocol using newly designed non-polymorphic primers, resistance allele frequencies (RAFs) were estimated in field populations of An. sinensis, Cx. pipiens and Cx tritaeniorhynchus collected from an identical site in Korea. The dynamics of each resistance allele frequency over time in the same populations were also evaluated.

Phthalate is an endocrine disruptor that interferes with homeostasis and developmental regulation. It is highly toxic to the environment and is associated with various diseases of the human body. Using biological samples from 140 adult subjects, to evaluate the influencing factors which are related to contaminant concentration levels, we used correlation analysis and multiple regression analysis. Lastly, in order to analyze the health effects related to exposure to phthalates, we conducted a risk assessment by estimating acceptable daily intake exposure according to the influential factors. When we compared the concentration level according to influential factors, in general, the subjects who had engaged in home remodeling work had higher urinary phthalate metabolite concentrations levels than the subjects who had not engaged in home remodeling work. We can confirm statistically significant differences in DBP metabolites. In addition, we can confirm the concentration appeared higher in the categories such as using air freshener, sofa and foods. Through conducting a risk assessment of DEHP, BBzP, DiBP, and DnBP by using data on phthalate metabolite concentration in urine, it was found that the average concentration of all metabolites did not exceed TDI.

Incursions of red imported fire ant (RIFA), Solenopsis invicta into Korea have been increasing. After a first interception of a colony of S. invicta on Gamman pier, Pusan port while intensive surveillance by Animal & Plant Quarantine Agency (APQA) in September 2017, three more RIFA colonies have been found in sea port piers of Pyeongtek, Incheon and Pusan cities. The social forms and mitochondrial DNA haplotypes of the intercepted RIFA colonies were analysed by allelic discrimination assay (peptide nucleic acid probe based RT-PCR) of Gp-9 gene and mt-DNA fragment of 768 bp, which is part of the Cytochrome oxidaseⅠ gene. The colony on Gamman pier, intercepted in Sep. 2017 was previously reported as a haplotype 5 (H5) of mitochondrial DNA and a social form of polygyne. The colony on Hutchison pier of Pusan port, intercepted in June 2018 were confirmed as a H22 haplotype and a monogyne. Those different social forms show different origins of each colonies. Those on piers of Pyeongtek and Incheon ports, also found in 2018 were confirmed samely as H22 and monogyne. However, it could be putatively assumed that those two colonies were differently introduced via different container cargoes, considering those colonies were found in container yards of distantly located different sea ports. More genetic variation analyses using diverse sets of molecular markers such as microsatellites, genome-wide single nucleotide polymorphisms, etc. in nuclear gens are being proceeded for more exact introduction routes (origins).

To identify the venom components and their expression patterns of some Aculeata bees/wasps, venom gland-specific transcriptome analysis was conducted. FPKM values were normalized with the average of the transcription level of reference gene (a-tubulin). Common components in both solitary and social wasp venoms include hyaluronidase, phospholipase A2, metalloendopeptidase, etc. Although it has been expected that more diverse bioactive components with the functions of prey inactivation and physiology manipulation are present in solitary wasps, the information on venom compositions of solitary wasps obtained in this study was not sufficient to generalizae this notion. Nevertheless, some neurotoxic peptides (e.g., pompilidotoxin and dendrotoxin-like peptide) and proteins (e.g., insuline-like peptide binding protein) appear to be specific to solitary wasp venom. In contrast, several proteins, such as venom allergen 5 protein, venom acid phosphatase, and various phospholipases, appear to be relatively more abundant in social wasp venom. In the venom gland trancsriptome of bumblebees, major allergens or pain producing factors were barely identified, implying that bumblebee venoms are relatively less toxic than those of social or solitary wasps.

본 연구는 간호대학생과 비 간호대학생의 문화적 역량과 심리적 안녕감 정도를 탐색하기 위한 서술적 조사연구이다. 본 연구를 위해 S와 I지역의 종합대학 간호대학생 127명과 비 간호대학생 124명, 총 251명을 대상으로 자료를 수집하였다. 연구 결과 문화적 역량 정도는 평균 5점 만점에 간호대학생 3.35점, 비 간호대학생 3.23점, 심리적 안녕감 정도는 평균 5점 만점에 간호대학생 3.53점, 비 간호대학생 3.37점으로 나타났으며, 두 군 모두 문화적 역량 및 심리적 안녕감 정도가 ‘중’ 수준 이상으로 나타났으며, 간호대학생이 비 간호대학생보다 유의하게 높았다. 문화적 역량과 심리적 안녕감 간의 관계 분석결과 간호대학생은 문화적 인식, 문화적 지식, 문화적 민감성, 문화적 기술, 문화적 경험, 자아수용성, 환경에 대한 통제, 긍정적 대인관계, 자율성, 삶의 목적, 개인적 성장 모든 항목에서 유의한 정적인 상관관계가 있었다. 따라서 간호대학생의 문화적 역량을 높은 수준으로 유지하도록 정규교육과정에 다문화교과목 편성 및 다문화현장체험, 특강 등 교과외 활동을 병행하여 심리적 안녕감을 가지고 문화적 다양성을 고려한 간호를 제공할 수 있도록 해야 할 것이다.

The common bed bug, Cimex lectularius, possesses a cholinesterase expressed exclusively in the salivary gland (ClSChE). In this paper, we investigated the molecular structure, tissue distribution patterns, and biochemical properties of ClSChE and showed that ClSChE exists as a soluble monomeric form or a soluble dimeric form connected by a disulfide bridge. Immunohistochemical analysis confirmed that ClSChE was expressed in the epithelial cells of both the salivary gland and the duct. In addition, the secretion of monomeric ClSChE through the proboscis during feeding was detected by western blotting using a ClSChE-specific antibody. To predict the role of ClSChE injected into the tissue of an animal host, we analyzed the extent of sequestration and hydrolysis of acetylcholine (ACh)/choline (Ch) by ClSChE by ultra-performance liquid chromatography-tandem mass spectrometry. Kinetic analysis revealed that ClSChE possesses extremely low Km (high affinity to ACh) and Vmax values. These findings suggest that ClSChE functions as a sequestering enzyme specific to ACh (not to Ch) by having a very strong affinity to ACh but an extremely long turnover time.

본 연구의 목적은 강성 개폐식 대공간 건축물의 비정형 입체트러스를 파라메트릭 기법을 적용하여 모델링을 구현하는데 있다. 개폐식 대공간 건축물은 비선형성 형상을 모델링하거나 다수의 모델을 대안별로 생성, 해석, 검토하는 과정에 많은 시 간과 기술이 소요되는 것이 현 실정이다. 이러한 문제점을 해결하기 위해서 첫째로, 개발에 사용된 파라메트릭 모델링 전산 도구를 소개하고, 강성 개폐식 대공간 건축물의 비정형 입체트러스를 분석하며, 개발한 입체트러스의 파라메트릭 컴포넌트 로 모델링을 구현하게 된다. 따라서 지붕이 개폐되는 방식으로 구분한 입체 트러스 종류의 모델링을 구현해 낼 수 있는 컴포 넌트를 형상이나 기능에 따른 각각 대안별로 개발하여 효율적인 개폐식 지붕의 모델링을 가능하게 하는 것이 추후 연구사항 이다.

The organic light-emitting diodes are fabricated with six anthracene derivatives containing simple substituents such as phenyl or naphthyl group. The device structure is as in the following: Indium tin oxide (ITO) (180 nm)/4,4-4,4`,4``-tris[N-(1-naphthyl)-Nphenylamino] triphenylamine (2-TNATA) (30 nm)/4,4`-bis[N-(1-naphthyl)-N-phenyl-1-amino] biphenyl (NPB) (20 nm)/Emitting compound (30 nm)/2,2′,2"- (1,3,5-Benzinetriyl)-tris (1-phenyl-1-H–benz-imidazole) TPBi (40 nm)/lithium quinolate (Liq) (2 nm)/Al (100 nm). In the emitting layer the anthracene derivatives are used without any dopant. All the six devices show blue emissions. Among the tested diodes, the one with 9-(2-naphthyl)-10-(p-tolyl) anthracene (2-NTA) exhibited luminous efficiency, power and external quantum efficiencies of 3.26 cd/A, 0.98 lm/A, 2.8 % at 20 mA/cm .

In this paper, it is covered in detail the process of generating structural alternatives with geometry change and its optimization by StrAuto. The main roof structure of the Exhibition Center is modelled parametrically and the optimal alt is derived by observing volume changes according to geometry change of main roof truss. Existing studies performed optimization process through sections and properties due to the limitations of shape change, but this study have meaning of performing the optimization with geometry changes which is the most critical skills of StrAuto. By the process of securing a sufficient margin by geometry changes and reducing volume with the optimization of sections, despite of a partial optimization of large space structure, it could be reduced by 11.7% of the total volume.