Histologic and immunohistochemical features of canine skin tumours originated from epithelial stem cells were described. The tumors were trichogenic trichoblastoma, trichoepithelioma, sebaceous adenoma, hepatoid gland adenoma and epithelioma, and basal cell tumour. All of the tumors expressed both AE1/AE3 and cytokeratin 5/8, whereas cytokeratin 7 and vimentin not at all. Neuron-specific-enolase was strongly positive in follicular tumours, whereas slightly in basal cell tumour. Moreover, chromogranin A was expressed slightly only in trichogenic trichoblastoma and trichoepithelioma. Since the morphological similarities between these tumors, immunohistochemistry is one of useful tools for differential diagnosis. From the immunohistochemistry data, we conclude that cytokeratins, vimentin and NSE play a main role in the evaluation of canine skin tumours originated from epithelial skin stem cells histogenesis.