Exposure to house dust mites is closely connected with allergic diseases such as atopic dermatitis, allergic rhinitis, and asthma. House dust mites (Dermatophagoides farinae and D. pteronyssinus) act as allergens even after their death. Therefore, repelling the house dust mites is more effective method than killing them. Many chemical agents have been used in killing house dust mite. However, it is usually reported that these chemical agents (acaricides) exhibit adverse effects and toxicity toward animals and human. For these reasons, we carried out the experiments for measuring the repellent activity of Mate tea and Jasmine tea against house dust mites in this experiment. In order to determine the concentration having the most effective repellent effect of Mate and Jasmine tea, house dust mites (D. farinae and D. pteronyssinus) were exposed at different concentrations (0, 0.015625, 0.03125, 0.0625, 0.125, 0.25, 0.5 mg/40 μL) of Mate tea and Jasmine tea extracts, respectively, for different hours (0.5, 1, 2, 3 hours). The most effective repellent effect (%) against house dust mite in 0.25 mg/40 μL of Mate tea for 2 hours was 73.5%, Jasmine Petal tea in 0.0625 mg/40 μL for 1 hour was 84.1% and Pearl Jasmine tea in 0.125 mg/40 μL for 0.5 hour was 82.8%. These results suggest that Mate tea and Jasmine tea extracts have potential effect to repel the house dust mites (D. farinae and D. pteronyssinus)

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

[n order to obtain the trlle expected DNA prod uct from PCR and RT-PCR using genornic DNA or cDNA reversely transcribed from mRNA. the PCR should be done in an appropriated condition. Sometimes the PCR was repeatedly fail ed. and cventllally the PCR product was turned out to be nonspecific and rudimentary . And more‘ t he PCR prodllctwas not reproducible even though careflll repeat of experiments. As the PCR was based on the exact primel hybridization. the condition of primer hybridization should be properly controlled by a nnealing temperatllre. But the selection of primer seqllences for targeting a specific gene is mostly important. A new method of primer eval uation is now available llsing DNA base pair polarity program. This study presents an example of PCR targeting to human Bax gene using genomic DNA. The DNA base pair polarity theory can di vide the genetic cord into propel DNA segments and calclllaLe their DNA base pair hybridization energy. Thus. mathematically the degree 0(' exact primer hybridization can be expected for the t r1l8 targeting of PCR. However, the DNA base pair polal'ityanalysis demonstrates that the more frequent number of DNA segment incl'eased the specificity of PCR. but decreased its sensitivity . While the greater polarity of DNA segment composed of increased nllmber of polarized DNA base pairs showed increased sensitivi ty 0 1' PCR. bllt relati vely decreased specificity of PCR. With the mllltiple analysis of PCR. especially for PCR cloning from the gDNA and cDNA, we found that the primers themselves showed secondary strllcture of partial hybridization between sameprimers or each pair primers. The DNA base pail‘ polarity signal can directly demonstrated symmetric sequences 0 1' each primer. and also can distinguish the dimmer formation from each pair primers. At least the symmetric seqllence of fOlll‘ base pairs dramatically showed the dimrner formation. On the other hand. in addi tion Lo the statlls of DNA base pair polarity the three-dimensional strllctllre of DNA dOllble helix targeted by the primer seqllences may affect the sensitivity and specificity of PCR detection. The present study introduced a new method of primer evalllation and selection in order to obtain abundant and exacL! y-trlle DNA product for genomic ffilltation analysis and gene expression profï le

Background : The purpose of this study was to big strong and root in the disease, many high quality seed of Lithospermum erythrorhizon selection by fostering, expanding the spread on a farm and Hongju subsistence production base and raw materials medicines of sources deployment for process is to contribute to increasing farm income. Methods and Results : Collected variety into the chemical mutation breeding method, Jecheon of Chungbuk, was conducted in 2001. In 2002 and 2003, two～ three individual selection in 2005 as it produces the 24 individual in 2004. From 2006 to 2010, pure line isolation as it cultivated and one foundation group. Promising in 2011, select one system. Replicated yield trial for two years beginning in 2012 a result of the stem length, providing high -quality “No. 1, Jeollanam-do” majority by assigning system name. Naju, Jangheung-gun, local excellence by implementing a local adaptability test recognized in two years. To breed a new variety named as a dwelling “ Daehong” of Lithospermum erythrorhizon the review by fostering december 2014 to be registered. Conclusions : Leaf type is the broad lancet, flowering time is new variety six days than Jecheon native variety fasted. New variety of stem length and stem thickness are check variety (87cm, 8.25mm) of 9cm tall and 0.6mm thick. Seed yields has native variety as much as 81 % compared to the many into 45.6 kg/10a. The shikonin contents of roots was 1.3 times higher compared to check variety (447 ㎍ / g). The dried root yields of the new variety are 221kg/10a a many native variety than 50%.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different covering material and weed eliminating on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of weed elimination with covering material were conducted under four condition compose to Non covering(4～6 times by manual weed control), Barley straw cutting covered with 700㎏․10 a−1, Rice straw cutting covered with 500㎏․10 a−1, Rice husks covered with 1,000㎏․10 a−1. Fermented mixed oil cake compost applied at 380㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Sowing dates were March 25, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : Rice husks covering < Barley straw covering < Barley straw covering cultivation this order, aerial part as a result were plenty amount of growth. Weed the rates was 12 to 29% lower incidence. Green leaf yields is uncovering control plot (915 kg․10 a−1) compared to 2% from covering rice husks, barley straw and rice straw covering increased to 18%, 20%, respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different organic fertilizer and applicated cultivation on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of application with organic fertilizer were conducted under nine condition compose to control(non application), N-P2O5-K2O (20-12-10)㎏․10 a−1, Hyphal incubation compost(HIC) applied at 851㎏․10 a−1, Hangawi organic compost(HOC) applied at 547㎏․10 a−1, Fermented mixed oil cake compost(FMOC) applied at 380㎏․10 a−1, 60% and 40% of basal fertilizer in March 12, top dressing were four application in April 12, May 12, June 13 and July 12, respectively. Sowing dates were March 23, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : chemical fertilizers < hyphal incubation compost < hangawi organic compost < fermented mixed oil cake compost application, top dressing than 60% in order of 40% additional fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Green leaf yields is untreated control plot (875 kg․10 a−1) compared to 12% from using chemical fertilizers, HIC application 18% higher, HOC application 20% higher, and FMOC application increased to 28% of the most.