Several studies on the correlation between temperament and genetic diversity are conducted in animals as well as human. Horse temperament is especially important because it is important factor for horse riding and racing. In this study, we performed targeted exome sequencing to find single nucleotide polymorphisms (SNPs) served as genetic markers that can evaluate the aggressive and docile levels of horses. We selected 71 candidate genes related to animal and human temperament through previous researches and verified it on the human reference genome (hg38) and horse reference genome (equCab2). We found that 16 orthologous genes were present in horse reference genome and 17 homologous genes found in horses based on the human reference genome. Finally, we designed probes to find the genetic variation in selected 33 genes. The sequencing libraries were constructed using the designed probe and DNA samples extracted from the blood of 8 aggressive and 8 docile horses. The constructed libraries were sequenced using the Illumina Hiseq2500 platform. SNPs data obtained from targeted exome sequencing will be used for genome wide association study (GWAS) and Sanger sequencing validation. This study will help to assess the horse temperament and to select superior horses for riding or racing.

Magnaporthe oryzae is a causal pathogen of the rice blast, the most destructive disease of rice worldwide. This ascomycete fungus has been intensively studied as a model organism in plant-pathogen interactions. Recently, the genome sequence of M. oryzae 70-15 was published by International Rice Blast Consortium. This led us to investigate comparative and functional analysis of this pathogen at genome-wide level. As a first step, sequencing project of M. oryzae Korean strain KJ201 was launched to face practical interests on diversity of plant pathogens. We constructed a fosmid library with a copy number controllable pCC1 vector. Over 13,000 end reads from 6637 fosmid clones were generated and anchored on genome sequence of the strain 70-15. Currently, 9,793 end reads, in which 2.684, clones with both ends and 1,757 clones with on end matched, are aligned to the sequence of strain 70-15. In the next step, shotgun sequencing of fosmid clones was conducted for fine scale comparative analysis of chromosome 7 that is well defined in genome sequencing of 70-15. Totally 119 fosmid clones were pooled in region and analyzed. For reasonable intra-species comparison in genome of M. oryzae, two strains, KJ201 and Guy11, were chosen for whole genome shotgun sequencing analysis with GS454 FLX system. About 8X genome coverage sequences were obtained for each strain and being merged for comparative analysis. Genome information of other strains is being also available from China and Japanese research groups and could be joined together for extended comparative analysis and the results give more detail in genome diversity in species level.