This study is the first report about the toxicity of pesticides to the mycophagous predator, I. koebelei, of powdery mildew of agricultural crops. Pesticides we tested are composed of synthetic and environmental-friendly products and being used conventionally for the control of insect or microbial pests on cucumber in Korea. our study was conducted to determine the relative toxicities of several pesticides used in Korea cucumber production to mycophagous natural enemy, I. koebelei and to provide a background for implementation of integrated powdery mildew management programs. Based on IOBC classification, three insecticides, bifenthrin + imidacloprid, acetamiprid + indoxacarb, acetamiprid + etopheprox are classified as having a Class 4 (harmful). Spiromesifen showed the low toxicity to the survival and the fecundity of I. koebelei when this chemical had been exposed to 3rd larva or newly emerged adult via feeding with cucumber powdery mildew. However, pyriproxyfen not only decreased the fecundity of female adult but also strongly prohibited from pupation. Many commercial biological or botanical pesticides can restrict the population of I. koebelei. However, Q pact (a.i. Ampelomyces quisqualis 94013), Top seed (a.i. Paenibacillus polymyxa AC-1), BT one (Bacillus thuringiensis) and Solbitchae (insecticidal microorganism) had no toxicity to I. koebelei when this chemical had been exposed to 3rd larva or newly emerged adult feeding with cucumber powdery mildew.

국내에서 절화장미의 생산에 있어서, 겨울철 부족한 광량과 저온은 생산량을 감소시키고 품질을 저하시킨다. 이에 일몰 후 고압나트륨등을 보광하여 생산량을 증가하고품질을 향상하기 위해 시험을 수행하였다. 절화장미 ‘챠밍블랙’, ‘핑키걸’ 품종을 사용하였으며 400W의 고압나트룸등을 이용해 35µmol•m−2•s−1의 광을 일몰 후 18시부터 4시간, 8시간, 12시간을 보광하였다. 고압나트륨등을 보광 하였을 때 온실내의 온도는 2~4oC가 상승하였고, 습도는 15~20%가 하락하였다. 고압나트륨 보광에 의한 온도의 상승과 습도의 하락은 절화장미 재배에서 노균병 발생을 억제하는 효과가 있었다. 보광시간이 길어질수록 절화장미의 노균병 발생율이 감소하였다. ‘챠밍블랙’은 8시간 이상의 보광처리구에서, ‘핑키걸’은 12시간보광 시 노균병이 100% 억제되었다. 절화장미에서 일몰후 보광시간이 길어질수록 블라인드의 발생률은 감소하였으나 생산량은 8시간 보광을 실시한 처리구에서 가장많았다. 절화장미의 절화장은 보광을 실시하였을 때 길어졌으며, 보광시간별 유의한 차이는 없었다.

Urbanization is one of the leading causes of habitat loss, habitat degradation, and fragmentation. Urban development negatively affects biodiversity. This study aimed to clarify the change of butterfly communities on effect of urbanization in urban green areas. Butterfly survey was conducted using the line transect methods from April to October in 2012. A total of 59 species and 1,465 individuals of butterflies were observed in four urban green areas: Namsan Park (NS), Ewha Womans University (EW), Bukseoul Dream Forest (BD), and Hongneung Forest (HF), and natural forest: Gwangneung Forest (GF). The category of land use around study site was determined based on GIS data. Species richness and abundance of niche breadth and habitat type in urban green areas differed significantly from those in GF. Estimated species richness and species diversity (H’) in four urban green areas were significantly lower than those in GF. Species richness and abundance of forest interior species and specialist were positively correlated with paddy, field, and forest, whereas those of forest interior species and specialist were negatively correlated with urban area and road. Butterfly communities in four urban green area differed from that in GF. The result suggests that the decrease of paddy, field, and forest associated with increase of urban area and road negatively influences species composition and changes butterfly communities.

To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-di-mensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the re-sults, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young pig-let separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was car-ried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age de-pendent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an im-portant source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differ-entiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphoryla- tion, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myo-genic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and PPARγ increased in rosiglitazone treatment. In study 3, we examined the effect of dexame-thasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and PPARγ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblasto-genic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90∼100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson’s, oil red O, and Alizarin red staining respectively. We per-formed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator- acti-vated receptor gamma (PPARγ) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteo-blast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were indu-ced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strat-egies for augmenting meat quality.

절화장미의 수송 중에 문제가 되고 있는 잿빛곰팡이병의 발생을 억제하기 위해 SO2가 발생하는 sodium metabisulfite(SMS) 2.5g/m3, 5.0g/m3, 7.5g/m3을 저장온도(상온: 24-28οC, 저온: 9- 12οC, 변온: 저온저장하면서 하루 2시간씩 상온에 노출)와 포장방법 (폴리에틸렌 비닐로 포장한 박스, 폴리에틸렌 비닐을 포장하지 않은 박스) 달리하여 상자의 상단부에 처리하였다. 저장온도에 의한 잿빛 곰팡이병의 발생률은 상온에서 가장 높았고, 변온, 저온 순으로 낮 아졌다. 수출용 상자를 밀폐하였을 때 잿빛곰팡이병의 발생률이 높았 고 발현시기도 1-2일 빨랐다. SMS의 처리량이 많을수록 상자 내 부의 SO2 농도는 높게 나타났으며, 잿빛곰팡이병의 발생률이 감소하 였다. 특히 7.5g/m3 SMS 처리시 잿빛곰팡이병의 발생은 무처리에 비해 20% 정도가 억제되었다. SMS 처리는 포장방법별, 온도별 처리 모두에서 잿빛곰팡이병의 발생을 억제하였으며 특히, 상온과 변 온에서 포장상자를 밀폐한 처리구에서 효과가 높았다. 이는 수송 중 온도 변화가 심한 하절기나 수송 중 컨테이너 중앙에서 공기의 이 동이 원활하지 않은 포장상자의 잿빛곰팡이병 발생억제에 효과가 높 을 것으로 사료된다.

The centipede Scolopendra subspinipes mutilans has been a medically important arthropod species by using it as a traditional medicine for the treatment of various diseases. In this study, we derived a novel lactoferricin B like peptide (LBLP) from the whole bodies of adult centipedes, S. s. mutilans, and investigated the antifungal effect of LBLP. LBLP exerted an antifungal and fungicidal activity without hemolysis. To investigate the antifungal mechanism of LBLP, a membrane study with propidium iodide was first conducted against Candida albicans. The result showed that LBLP caused fungal membrane permeabilization. The assays of the three dimensional flow cytometric contour plot and membrane potential further showed cell shrinkage and membrane depolarization by the membrane damage. Finally, we confirmed the membrane-active mechanism of LBLP by synthesizing model membranes, calcein and FITC-dextran loaded large unilamellar vesicles. These results showed that the antifungal effect of LBLP on membrane was due to the formation of pores with radii between 0.74 nm and 1.4 nm. In conclusion, this study suggests that LBLP exerts a potent antifungal activity by pore formation in the membrane, eventually leading to fungal cell death.

We carried out DNA barcoding of five Korean Lymantria species to establish identification references library for quarantine inspection. Total of 118 samples including 34 samples obtained through quarantine inspection, two from USDA, and one collected from Philiphine were used for this study. And 30 sequences of 10 species from GenBank of NCBI were used as reference sequences. In a result of DNA barcoding of the Korean Lymantria species, sequence divergence of 148 DNA barcodes ranged from null to 17.0%, intraspecific divergence from null to 1.0%, and interspecific divergence from 5.1 to 17.0%. In NJ tree, L. dispar contained three clusters, which were identified as L. dispar asiatica, L. albescens, and L. xylina, respectively. L. xylina was collected through quarantine inspection on a foreign merchant ship in Yeosu port, and L. albescens was obtained by pheromone trap on L. dispar installed in Busan port. And L. monacha known as single species in Korea was revealed as species complex with three species, L. monacha, L. minomonis, and L. sugii. In subspecies level, L. dispar dispar (EGM) built single cluster, but L. d. asiatica (AGM) and L. d. japonica showed as multiple cluster. Therefore, DNA barcoding lead to rapid and accurate identification in species level, but in subspecies level, only a taxon showing geographically far distance was discriminated from the others. And the results could provide a taxonomic outline of the Korean Lymantria fauna and might be used as identification reference for Lymantria species in quarantine inspection.

A taxonomic review on the Korean Lymantria Hübner, 1819 was conducted. In a result, a total of nine species under four subgenera including two new recorded species were detected as followings: L. dispar asiatica Vnukovskij, 1926, L. xylina Swinhoe, 1903, L. monacha (Linnaeus, 1758), L. minomonis Matsumura, 1933, L. sugii Kishida, 1986, L. lucescens (Butler, 1881), L. mathura Moore, 1865, L. fumida Butler, 1877, and L. bantaizana Matsumura, 1933. Of the two unrecorded species, L. minomonis was found only in Is. Bogildo of Jeollanam-do, the southern part of Korea, and the other one, L. sugii was collected in the middle part of Korea. On the two species, L. xylina and L. fumida, the Korean specimens could not be examined through this study. Therefore, we considered that the two species might be excluded from the Korean Lymantria fauna. Each species was identified on the basis of wing pattern and genitalia of male/female adult. We provided diagnosis, male/female adult habitus photos, male genitalia photos, and female ovipositor photos.

The purpose of this study is to analysis of muscle fatigue in the upper trapezius and splenius capitis muscles according to therapy table height variation. The subjects were consisted of 15 healthy adults(10 males, 5 females) who had no medical history of neurological and musculoskeletal problems. In experiment, wireless electrode EMG system was measured for each the upper trapezius and splenius capitis muscles during the treatment performed on table. the differences in the muscle fatigue was compared for 4 types of table height(-6cm, -3cm, 0, +3cm from elbow in 90° flexion position). Muscle fatigue according to therapy table height were significant difference except for left upper trapezius. And muscle fatigue of right upper trapezius and splenius capitis showed significant decrease in +3cm table height compared to -6cm table height(p<.05). Muscle fatigue of right upper trapezius and splenius capitis were the highest in -6cm table height, but those were the lowest in +3cm table height. This study propose to change therapy table height higher than +3cm from elbow in 90° flexion position, if you hope to reduce muscle fatigue.

본 연구는 환경스트레스 저항성이 증진된 페튜니아를 개발하기 위하여 NDPK2유전자 도입 형질전환 계통 NDPK2-7-1와 SOD2 유전자 도입 형질전환 계통 SOD2- 2-1-1-35간의 교잡에 의해 획득된 후대들의 비생물적 스트레스 저항성을 조사하기 위해 수행되었다. 비 생물적 스트레스 유발원인 메틸바이올로젠(methyl viologen, MV) 100 μM과 200 μM 처리에서 교잡후대들은 그들의 교배 모본 SOD2 유전자나 NDPK2 유전자가 단독으로 도 입된 형질전환 계통이나 비형질전환체 보다 메틸바이 올로젠에 의한 피해를 적게 받았다. 이는 SOD2 유전 자나 NDPK2 유전자가 단독으로 도입된 형질전환 계 통간 교잡에 의해 획득된 후대들이 그들의 교배모본 (SOD2 유전자나 NDPK2 유전자가 단독으로 도입된 형질전환 계통)이나 비형질전환체 보다 산화적 스트레 스에 대한 저항성이 증진되었음을 증명해 준다고 할 수 있다. 이들 교잡후대들은 초장 등 11종류의 양적형질의 특성이 비형질전환체에 비해 약간 길거나 짧긴 하였지 만 비형질전환체와 거의 유사하였으며, 꽃 색갈이나 모양 또한 그들의 교배모본 (SOD2 유전자나 NDPK2 유전 자가 단독으로 도입된 형질전환 계통)이나 비형질전환 체와 차이가 없었다.

The purpose of this study is to examine the effects of gait training using functional electrical stimulation on the improvement of hemiplegic patients' functions for balance and gait velocity. The subjects of the experiment were determined to be 10 each hemiplegic patients who had been diagnosed with stroke or brain damage six months or longer earlier assigned to an experimental group and a control group respectively. The subjects were evaluated before the experiment using Tetrax and 10M gait tests, received gait training five times a week for four weeks using functional electrical stimulation and were evaluated after the experiment in the same method as used in the evaluation before the experiment. In order to examine differences between the experimental group that received gait training using functional electrical stimulation and the control group that was treated by functional electrical stimulation and received gait training thereafter, differences between before and after the experiment were analyzed using paired sample t-tests and differences in changes after the experiment between the experimental group and the control group were analyzed using independent sample t-tests in order to compare the two groups with each other. Experimental results showed significant differences in weight bearing, balance and gait velocity between before and after the experiment in the experimental group(p<.05). In the control group, whereas weight bearing and gait velocity did not show any significant difference between before and after the experiment(p>.05), balance showed significant differences(p<.05). Weight bearing, balance and gait velocity change rates showed significant differences between the experimental group and the control group(p<.05). In conclusion, it was indicated that gait training using functional electrical stimulation is effective for enhancing stroke patients' weight bearing rates, balance abilities and gait velocity.

Testes‐derived unipotent male germ‐line stem (GS) cells can acquire multipotency under appropriate culture conditions to become mGS cells which can contribute to all three germ‐layers. This study was designed to investigate the epigenetic characteristics of mGS cells derived from adult mouse testes (maGS cells). The GS cells were isolated from 4 6 week DBA mouse and were cultured in Dulbecco’s modified Eagle Medium supplemented with 15% (v/v) fetal bovine serum, 1,000 U/ml LIF, 4 ng/ml GDNF at 37℃ in an humidified atmosphere of 5% CO2 in air to derive the maGS cells. The multipotency of maGS cells were verified by morphological and gene expression analyses, teratoma formation upon transplantation into nude mouse and in vitro differentiation ability. Bisulfite genomic sequencing revealed that GS cells had androgenetic DNA methylation pattern at the Igf2‐H19, Gnas‐Nespas , and Dlk1‐Dio3 imprinted gene clusters which changed to hemi‐zygotic embryonic stem (ES)‐cell like pattern in the maGS cells. Western blot analysis, using modification‐ and residue‐specific antibodies, revealed that both maGS and ES cells had similar level of histone di‐methylation at 4th and 27th lysine residue of histone 3 (H3K4me2 and H3K27me2) which represent “bivalent domain” for regulating self‐renewal and differentiation of mouse ES cells. Both maGS and ES cells also shared similar hisone modification for H3K9me2, H3K79me2, H3K9ac and H3K18ac. However, maGS cells had higher level of H3K- 36me2 and H3S10p. These data suggest that maGS and ES cells share several epigenetic characteristics but they also have their own unique epigenetic marks that may be useful as a molecular marker for their identification.