We investigated the distribution and habitat characterization of Korean Osmia spp. in apple orchard of 32 localities during 2005 to 2008. A total of 6,737 Osmia spp. were collected, consisting of six species, which was Osmia cornifrons, O. pedicornis, O. taurus, O. benefica, O. satoi and O. jacoti. The rate of bee nested was 11.7%. Among collected 32 localities, Choongju showed the most collecting rate among other localities, which was 22.4% and 17.5% for Yeongju and 14.9% for Geochang. In case of collected six Osmia spp, O. cornifrons was 60.2%, which was the dominant species and O. pedicornis was 20.4% and O. taurus was 10.8%, respectively. In case of sex ratio, O. cornifrons was 1 : 2.7 and 1 : 5.6 for O. pedicornis and 1 : 2.3 for O. taurus. Osmia spp. was more collected at habitat conditions within apple orchard with plentiful pollens place and abundant water.