The purposes of this study are to analyze risks of construction step BIM(Building Information Modeling) applied project and improve it. Recently, construction industry has emphasized an importance of BIM for efficient utilization of various information. In the whole life cycle of huge construction project, there are cases introducing BIM and projects applying BIM are gradually increased to the work of construction step. However, the process of cooperation is not established, so the contents of BIM process of construction step are just showing rough fields and concepts of working utilization, so it's restrictive to utilize BIM actively on constructions step. Through results of case study in this research, Construction company risks of BIM business should be efficiently treated with Fuzzy-FMEA that is more precise than existing danger evaluation.

In the construction sites, rush (speedy construction) work have recently been overused because of budget reductions by reducing the construction duration after the long-term economic recession and construction business stagnation with institutional change including the instruction of the duration shortening bidding system and the introduction of the post-construction sales system by the Korea Research Institute for Human Settlements. Due to this, negligent accidents have frequently occurred by forcing manpower, equipment, finance, and material procurement and pressing ahead with impractical construction wok at night according to the reduction of construction schedules. Therefore, this paper has the necessity of this study to seek for alternatives to prevent negligent accidents by finding factors related to rush work in the reality that the importance for preventing negligent accidents is more emphasized while they have not been reduced in construction sites.

Transposon elements are widely distributed in the plant genomes. Maize genome consists of various transposable elements that constitute as much as 80% of the genome. Transposon display (TD) is a modified from AFLP, and can be used to generate and display hundreds of genomic fragments affixed to transposons, consequently tagging transposons. We developed maize specific transposon insertion- sequence characterized amplified regions (Ti-SCAR) using Isaac and CACTA transposons. Currently, we have developed 58 dominant Ti-SCAR markers. Validation of the Ti-SCARs is being carried out using a various maize germplasm. Since AFLP is tedious and unsuitable for large scale application in MAS, the Ti-SCAR markers displayed simple binary presence or absence pattern. Thus, the Ti-SCARs can provide a fast, cheap and reliable PCR based assay. A pipeline in developing the Ti-SCAR will be presented in the poster.

EST-SSRs were developed in Brassica napus by database mining. We isolated 7,802 EST-SSRs from the B. napus 643,946 ESTs deposited in the NCBI. With the cut-off value of >10 repeats in di-nucleotide repeats and >7 tri-nucleotide repeats, 303 ESTs were suitable for primer designing for PCR amplification. Of the sixteen possible di-nucleotide combinations, only three types of repeats (AC/GT, AG/CT, and AT/TA) were present among the di-nucleotide EST-SSRs. Whereas, 27 tri-nucleotide repeat motifs from the 64 possible combinations were present. The repeat numbers ranged from 10-15 in di-nucleotide repeats and 7-9 in tri-nucleotide repeat motifs, respectively. By checking PCR amplification in 10 Korean rapeseed breeding lines or cultivars, 234 primer pairs showed successful PCR amplification and 142 of the 234 primer pairs revealed polymorphism among the control cultivars or breeding lines. While the repeat length does not related with the SSR polymorphisms, the repeat motif number showed positive relation with the polymorphism generation by showing higher repeat numbers with higher polymorphisms. We are here presenting the PCR amplifiable primer sequences of the 234 SSR primer pairs to aid in the germplasm management and breeding programs of the B. napus in Korea.