Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

This study investigated the effects of axillary crutch length on trunk muscle activity and lumbo-pelvic-hip complex movements during crutch gait. Eleven healthy men participated in this study. The participants performed a three-point gait with optimal, shorter, and longer crutch lengths. Weight-bearing (WB) side was determined as the dominant leg side. The electromyography (EMG) activity of the bilateral rectus abdominis (RA) and erector spinae (ES) muscles and lumbo-pelvic-hip complex movements were monitored using a three-dimensional motion system with wireless surface EMG. Differences in the EMG activity of RA and ES muscles and range of motion (ROM) of lumbar spine, pelvis, and hip among conditions were analyzed using one-way repeated-measures analysis of variance, and a Bonferroni correction was conducted. There was less RA muscle activity on the WB side under the optimal crutch length condition compared with shorter and longer crutch length conditions (p<.05). The EMG activity of the RA muscle on the non-WB side and ES muscle on the WB side were significantly decreased under the optimal crutch length condition compared with shorter crutch length condition (p<.05). No significant differences in the EMG activity of the ES muscle on the non-WB side and ROM of lumbo-pelvic-hip complex were found among conditions (p>.05). These findings indicate that the optimal crutch length improves the trunk muscle efficiency during crutch gait.

본 연구는 강우시 불투수성 지역에서 비점오염원 유출 특성을 분석하기 위하여 수행되었다. 불투수성 지역의 강 우사상 특성은 강우강도에 대한 영향을 많이 받는 것으 로 나타났으며, 강우강도가 크게 작용하면, 초기강우 현 상이 잘 일어나는 것으로 나타났다. 또한 선행무강우일수 에 의해서 비점오염물질의 농도차가 큰 것으로 나타나, 이에 대한 대책 마련이 필요할 것으로 판단된다. 불투수 성 지역의 비점오염원 유출 경향을 보면, 강우초기 유출 유량에 포함되어 유출되는 오염물질의 농도가 상대적으 로 매우 높게 나타났고, 일정 시간 경과 후에는 지속되는 강우에도 불구하고 오염물질의 농도는 증가하지 않고 낮 은 농도로 안정적인 유출경향을 나타내었다. 이러한 이유 는 건기 시 도로 표면에 집적되어 있던 오염물질들이 강 우시 초기에 유출되어 비점오염물질의 농도가 높게 나타 나는 것으로 나타났다. 불투수성 지역에서 발생하는 비점 오염물질 항목별 단위면적당 오염부하량은 매우 높은 값 을 나타내고 있는데, 이는 불투수성 지역의 특성상 아스 팔트로 되어 있는 도로 및 주차장, 교량 등이 있는 불투 수면이 대부분의 토양상부를 덮고 있기 때문에 강우시 불 투수성 지역 내 모니터링 지점에서 강우가 집중되어 유 출되기 때문에 비점오염물질의 유출이 증가하기 때문에 오염부하량이 증가한 것으로 판단된다. 강우시 초기세척 효과는 TSS의 경우, Event 1, Event 2에서 초기세척효과 가 매우 잘 관찰되었으며, TSS¤CODMn¤T-P 순으로 뚜 렷하게 나타나는 것으로 조사되었다. TSS와 CODMn가 가 장 민감하게 강우에 영향을 받는 것으로 나타났다. 이에 본 연구 결과를 통해 분석된 결과를 토대로 비점오염원 유출특성에 대한 다양한 데이터베이스를 구축하여 향후 비점오염원을 효율적으로 관리하는데 자료로 활용 가능 할 것으로 판단된다. 또한 비점오염원을 체계적으로 관리 하여 수계환경의 오염부하를 줄일 수 있는 방안을 강구 해야 할 것으로 사료된다.

This study was designed to investigate the effect of different hand positions on scapulothorcic muscle activities during push-up plus exercises. Fourteen healthy males performed push-up plus exercises under three conditions (neutral, internally rotated, and externally rotated hand positions), during which the activities of the serratus anterior, pectoralis major, and upper trapezius muscles were recorded using surface electromyography. The statistical significance at three different hand positions was tested by repeated one-way ANOVA. The mean activities of the serratus anterior increased and the mean activities of the pectoralis major decreased in the order of neutral hand position, internally rotated hand position, and externally rotated hand position. There was a significant difference during push-up plus between neutral and externally rotated hand positions as well as in the serratus anterior/pectoralis major activity ratio (p<.0.5). However, no significant differences were found in the activity of the upper trapezius muscle or the serratus anterior/upper trapezius activity ratio. We suggest that the push-up plus exercise performed in the externally rotated hand position could a beneficial strategy for selective strengthening of the serratus anterior muscle, while minimizing the activity of the pectoralis major muscle.