Calcium-binding proteins, like calcineurin B-like (CBL) proteins, represent important roles in plant calcium signaling. Calcium signals mediate a multitude of plant responses to external stimuli and regulate a wide range of physiological processes including pathogens, abiotic stresses and hormones. These proteins form a complex network with their target kinases being the CBL-interacting protein kinases (CIPKs). CBL genes play vital roles in multiple abiotic stress response pathways whereas some of these are more specifically involved in mediating ABA signaling. In this study, we collected 17 CBL genes designated as B. rapa CBL (BrCBL) from the Brassica database and analyzed the sequences. In comparison analysis, these genes showed high homology with published CBL genes of other species. An organ specific expression of these genes was observed in different organs of chinese cabbage plants. In addition, six BrCBL genes showed responsive expression after cold and drought stress treatments at certain time courses. All these data revealed that these CBL genes might be useful resources in developing abiotic stresses resistance Brassica.