Carbon quantum dots (CQDs) are novel nanocarbon materials and widely used nanoparticles. They have gradually gained popularity in various fields due to their abundance, inexpensive cost, small size, ease of engineering, and distinct properties. To determine the antibacterial activity of metal-doped CQDs (metal-CQDs) containing Fe, Zn, Mn, Ni, and Co, we chose Staphylococcus aureus as a representative Gram-positive strain and Escherichia coli as a representative Gram-negative bacterial strain. Paper disc diffusion tests were conducted for the qualitative results, and a cell growth curve was drawn for quantitative results. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and IC50 were measured from cell growth curves. As a result, all of the metal-CQDs showed toxicity against both Gram-positive and Gram-negative bacteria. Furthermore, Gram-negative bacteria was vulnerable to metal-CQDs than Gram-positive bacteria. The toxicity differed concerning the type of metal-CQDs; Mn-CQDs exhibited the highest efficacy. Hence, this study suggested that CQDs can be used as new nanoparticles for antibiotics.