The major limiting factor of the reproductive performance on many farms is the failure to detect estrus in a timely and accurate manner. The estrus detection for Hanwoo cows (Bos taurus coreanea) is generally performed by the visual observation which is estimated at about 40% detection rate only. This recurring problem among farmers has motivated the development of various estrus detection aids. The mounting event duration threshold, standing heat event frequency threshold, estrus detection algorithm and so on, are necessarily needed in order to detect the estrus of Hanwoo cows accurately when using some automatic devices. However, they are not yet investigated for Hanwoo cows. Therefore, the objective of this study was to establish a mounting event duration threshold and standing heat event frequency threshold more specifically for Hanwoo breed by discriminating the duration of standing heat mounting from shorter duration spurious mountings which routinely occur in cattle herds. One hundred and fifty Hanwoo cows with an age between 2 to 5 years were used in this study. Cows that were expected to come into estrus within 3days were randomly assigned to each compartment for the estrus group (EG). The heated cows were video recorded for about 24 hours until after post estrus. The results showed that Hanwoo cows can be considered on estrus when it stand immobile during mounted by any other cow in more than or equal to 3.15s and 3.22s in chest-tail head mount (CTM) and head-above back mount (HBM) and that occurs consecutively at least three times within 876.4s interval. The algorithm was also developed using the thresholds of the mount duration, mount interval and consecutive occurrence number.