Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP × Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (|log2FC| > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

본 연구에서는 파트린드 저수지의 유입부에서 댐 구조물까지 대상구간으로 설정하고 상류유입 유량, 유사 및 수문 운영에 따른 장기 퇴적양상을 EFDC 모형을 이용하여 5년에 걸쳐 모의하고 배사문 운영을 통한 배사효과에 대하여 모의하였다. 배사문 운영결과 배사시 수로를 형성하여 주로 수로를 따라서 활발히 발생하는 것으로 나타났으며 이는 기존 저수지 배사의 물리적인 특성을 잘 반영하는 것으로 판단된다. EFDC 모형의 모의결과로부터 취수구 전단부에서의 입도분포와 특정입경의 유사도달 여부 등 다양한 정보를 얻을 수 있었으며 향후 저수지 운영에 기초자료로 활용할 수 있을 것으로 기대된다.