All kinds of crops including foods, feeds and turf grasses are damaged frequently by various environmental stresses such as drought, salt, cold, and high temperature, which cause the loss of agronomic productivity. Plants cannot escape from environmental stresses. Thus, plants were evolving in the direction of overcoming environmental stresses. Some genes such as ARF, AB13, NAC, HSF, WRKY respond to environmental stresses have been reported in plants. The genes play a role in stress responses pathway of plants, the transcription factor in response to environmental stress. Typically OsWRKY76 increased the low temperature resistance, AtWRKY28 been reported to be related to the environmental stress. Zoysiagrass (Zoysia japonica Steud.) is used primarily useful for the garden or the golf course. But WRKY, environmental stress-related gene, is unknown in zoysiagrass. Here, we report the analyzing of WRKY genes and response by cold, dehydration and senescence stresses in zoysiagrass. Three WRKY gene (ZjWRKY3, ZjWRKY5, ZjWRKY7) cloning from zoysiagrass. It was transformed in arabidopsis and zoysiagrass. It will be a function analysis.