Bee pollen have been used for centuries as a kind of traditional medicine and food diets additive due to their nutritional and biological properties. The present study was to evaluate the biological features of ethanol extracts of 18 different bee pollen (EEBP) samples collected from 16 locations of South Korea. Our results indicated most of the EEBP samples showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, inhibitory activities against human β-amyloid precursor cleavage enzyme (BACE-1), and antiproliferative activities towards five cancer cell lines. However, these EEBP samples did not present human acetylcholinesterase (AChE) inhibitory activities, and intestinal bacteria growth inhibitory activities.