Background: The Nordic hamstring exercise is effective as a representative exercise for strengthening eccentric contraction that can activate the hamstring at high levels, improve eccentric contraction strength and ability, and significantly reduce injury rates. Objectives: To investigated a comparison with the lunge exercise to determine the effect of the Nordic hamstring exercise on muscle activity and balance ability in a sample of adult amateurs and football players. Design: Randomized controlled trials. Methods: The subjects of the study were stratified samples of 28 professional and amateur football players and two groups (Nordic hamstring group and lunge group) of 14 people each were studied. Surface electromyography using, was performed to measure the hamstring muscle activity, and the good morning exercise was selected as the measurement exercise. For balance ability, the surface area ellipse and length were measured with BioRescue, while for static balance, the posture of closing the eyes and single-limb standing was measured, and for dynamic balance the one-legged squat was measured. Results: The %MVIC of the semitendinosus and the biceps femoris improved in the Nordic hamstring exercise group and showed statistically significant increase (P<.05). The static and dynamic balance improved in the Nordic hamstring exercise group and showed statistically significant decrease (P<.05). Conclusion: The Nordic hamstring exercise can be useful as an exercise to improve the hamstring muscle activity and balance ability of professional and amateur football players.