Honey bee plays an important role in pollinating plants. Recently, however, declines in honey bee populations have been reported in many countries, and pesticides have been pointed out as one of the factors contributing to honey bee loss. To determine the effects of pesticides on honey bee behavior, we investigated the homing ability of honey bee exposed to four pesticides (acetamiprid, imidacloprid, fenitrothion, and carbaryl). In addition, the changes in expression levels of genes associated with ‘learning and memory’ (cGMP-dependent protein kinase foraging, Kruppel homolog 1, Adenlyate cyclase 3, Early growth response protein 1, Hormone receptor 38) were examined after pesticide treatment in forager bee. The four pesticides tested in this study generally reduced the homing ability of foragers. In the examination of gene expression, learning and memory-related genes were induced by the exposure to acetamiprid, imidacloprid, and carbaryl, whereas fenitrothion decreased the expression of these genes in honey bee. Although further studies are needed, this suggests that pesticides may have negative effects on honey bee behavior and behavior-related gene expression.